Significance changes in the levels of myocardial enzyme in the child patients with Mycoplasma Pneumoniae Pneumonia

Qi, Xueliang; Sun, Xiangguo; Li, Xuemin; Kong, Dejin; Zhao, Luhua

doi:10.14715/cmb/2020.66.6.8

Cited by 6 publications

(7 citation statements)

References 18 publications

(15 reference statements)

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“…In recent years, MP showed a yearly upward trend in the detection of pneumonia, this study reported the frequency of MP in pediatric patients diagnosed with CAP was identified in 21.0%, which was similar to several reports [44,45], however, other studies report lower frequencies [46,47]. 33.0% of HBoV-positive patients co-infected with MP in our research, compared with the other group who did not co-infected with MP in HBoV infection children, lower level of AST was found, but a study showed that Mycoplasma pneumoniae pneumonia patients in the acute phase had higher levels of AST than those of the patients in the recovery patients and healthy children [48]. It was also reported that AST level of patients with Chlamydia pneumonia was higher than that of Mycoplasma pneumonia [49].…”

Section: Variablesupporting

confidence: 90%

Epidemiologic and clinical characteristics of human bocavirus infection in infants and young children suffering with community acquired pneumonia in Ningxia, China

Sun

Yan

et al. 2021

Virol J

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Background Pneumonia has a high incidence rate and is a major cause of mortality in children, mostly community-acquired pneumonia (CAP). Human bocavirus (HBoV), since it first identified in 2005, has been repeatedly associated with respiratory tract infections. Nevertheless, the role and related information of HBoV as a pathogen of CAP has not been fulfilled. Here our study is to assess the epidemiological and clinical features in HBoV-positive children with CAP. Methods A total of 878 secretions of lower respiratory samples were obtained, multiplex PCR was used to detect HBoV and other respiratory viruses. Results Of all cases, HBoV was detected in 10.0%, with a peak incidence of infection among children < 2 year old, and predominantly noted in autumn and winter. Only 8 patients were HBoV single infection. Co-infection with other respiratory viruses was observed in 86.4%. Moreover, co-infection with bacteria occurred in 27.3% and with Mycoplasma pneumoniae (MP) in 33.0% of HBoV-positive patients. Among all HBoV-positive samples co-infected with bacteria, 87.5% are gram negative bacteria. Compared with HBoV-negative group, age (P = 0.048), wheezing (P = 0.015), tachypnea (P = 0.016), lactate dehydrogenase (P = 0.026) and severe pneumonia (P = 0.023) were statistically significant in HBoV-positive patients. Furthermore, HBoV-positive patients less than 1 year old were more likely to have co-infection with bacteria (P = 0.007). Conclusions HBoV can be detected alone in respiratory samples of children with CAP, maybe it is one of the causes of CAP in infants. The high incidence of severe pneumonia was found in HBoV-positive patients compared with HBoV-negative cases may indicate a relationship between severe pneumonia and HBoV.

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Section: Variablesupporting

confidence: 90%

Epidemiologic and clinical characteristics of human bocavirus infection in infants and young children suffering with community acquired pneumonia in Ningxia, China

Sun

Yan

et al. 2021

Virol J

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“…In addition, the results are easily affected by the status of pediatric patients with previous infection. Reportedly, some disorder occurs in humoral immunity and cellular immunity in children infected with MP, which is directly proportional to the infection status, indicating the occurrence of MP may be associated with the immune mechanism 4 …”

Section: Introductionmentioning

confidence: 99%

Serum amyloid a, C‐reactive protein, and procalcitonin levels in children with Mycoplasma pneumoniae infection

Jiang

Wang

Zhang

et al. 2022

Clinical Laboratory Analysis

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Background Mycoplasma pneumoniae (MP) is a common pathogen of community‐acquired pneumonia in children. In the present study, serum amyloid A (SAA), C‐reactive protein (CRP), and procalcitonin (PCT) levels in children with MP infection were analyzed and the differential diagnoses of MP evaluated. Methods The study included 152 children with MP infection hospitalized in Tai’an Central Hospital in Shandong Province and 50 healthy children as controls. SAA, CRP, and PCT, as well as serum immunoglobulins and T lymphocyte subsets were analyzed during the acute and convalescent phases. Among the MP‐infected children, 30 cases were selected to monitor the SAA, immunoglobulins, and T lymphocyte subset levels for a week. Results The SAA, CRP, PCT, IgA, and IgM levels were significantly higher in the MP‐infected group than in the control group (F(SAA) = 83.91, p < 0.05; F(CRP) = 40.79, p < 0.05; F(PCT) = 60.58, p < 0.05; F(IgA) = 43.45, p < 0.05; F(IgM) = 233.88, p < 0.05). In addition, the levels of these factors were significantly higher in the acute phase than in the convalescent phase (p < 0.05). However, significant difference was not observed in the IgG level between these two groups (p > 0.05). The CD3+ and CD4+ levels in the MP‐infected group were lower than in the control group ( F(CD3+)= 60.58, P < 0.05; F(CD4+) = 89.05, p < 0.05), and the CD8+ level was higher than in the control group ( F(CD8+)= 96.96, p < 0.05). The CD3+, CD4+, and CD8+ levels were significantly different between the acute phase and the convalescent phase (CD3+: acute phase vs. convalescent phase, q = 2.79, p < 0.05; CD4+: acute phase vs. convalescent phase, q = 2.83, p < 0.05; CD8+: acute phase vs. convalescent phase, q = 3.15, p < 0.05). The changes in serum SAA levels in the MP‐infected group positively correlated with the changes in IgA, IgM, and CD8+ levels and negatively correlated with CD3+, CD4+, and CD4+/CD8+. Conclusion SAA, CRP, and PCT were specific markers for diagnosing early MP infection in children. These findings are important in the differential diagnosis of MP infection and clinical guidance for MP treatment.

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“…When we analysed the correlation between the miR-10a-3p expression level and laboratory inflammatory indicators, we found a positive correlation between miR-10a-3p expression level and WBC, ESR, and CRP. Indicators such as WBC, ESR, and CRP play a role in the evaluation of pneumonia and the determination of treatment efficacy and are associated with progression of pneumonia in a certain sense (9,17). These results suggest that upregulation of miR-10a-3p expression in paediatric MPP might participate in disease development and progression.…”

Section: Changes In Expression Levels Of Nf-κb Signalling Pathwayrelated Proteins and Crp In Thp-1 Cells After Transfectionmentioning

confidence: 88%

“…They have high specificity in the regulation of their target genes. In addition, due to the time and tissue specificity of their regulation, miRNAs can perform very precise regulation of many biological processes (9). The expression levels of miRNAs are altered in various diseases, including autoimmune diseases, cancer, and infectious diseases, and they are associated with the pathogenesis of the diseases (2,8,10).…”

Section: Introductionmentioning

confidence: 99%

The role of targeted regulation of COX11 by miR-10a-3p in the development and progression of paediatric mycoplasma pneumoniae pneumonia

Ding¹,

Zhao²,

Xiong³

et al. 2021

J Thorac Dis

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Background: MiR-10a-3p is associated with the pathogenesis of many immune inflammatory diseases including Mycoplasma pneumoniae pneumonia (MPP), and cytochrome coxidase assembly homologue 11 (COX11) is one of its direct target proteins. This study investigates the function and mechanism of miR-10a-3p targeting with COX11 in the development and progression of paediatric MPP. Methods: Ninty-seven paediatric MPP patients and 100 age-and sex-matched healthy children were enrolled. Clinical and laboratory indicators of paediatric MPP patients were collected. The mRNA levels of the COX11 gene and miR-10a-3p were detected by qRT-PCR. THP-1 mononuclear macrophages were stimulated using MPP lipid-associated membrane proteins (Mp-LAMPs). The relative expression level of miR-10a-3p was detected after 12, 24, and 48 h. THP-1 cells were transfected to overexpress or inhibit the expression of miR-10a-3p, miR-10a-3p, COX11 mRNA, NF-κB signalling pathway-related proteins, and C-reactive protein (CRP) were detected after 48 h by Western blot. Results:The relative expression level of miR-10a-3p in the MPP group was 2.38±0.52, compared with 1.76±0.38 in control group (t=4.584, P<0.001) whileCOX11 in MPP group was 3.70±1.12, compared to 5.78±1.84 in control group (t=4.876, P<0.001). Pearson correlation analysis showed that miR-10a-3p and COX11 in MPP group presented a negative correlation (r=-0.679, P<0.001). By searching in the prediction website of TargetScan database, it was found that miR-10a-3p and Cox11 genes had targeted regulatory binding sites, and the targeting relationship between miR-10a-3p and Cox11 genes was confirmed by dual luciferase reporting assay in 293T cells. Among paediatric MPP patients, miR-10a-3p expression had a positive correlation with the white blood cells count, erythrocyte sedimentation rate (ESR), and CRP expression, while COX11 mRNA expression had a positive correlation with ESR and CRP. After LAMP stimulation, the miR-10a-3p expression level in THP-1 cells significantly increased (P<0.05). After THP-1 cells were transfected with the miR-10a-3p mimic or inhibitor, the relative expression level of miR-10a-3p significantly increased or decreased, respectively. COX11 expression in the mimic group significantly decreased, whereas COX11 in the inhibitor group significantly increased (both P<0.05). In addition, after transfection, IκBα expression significantly decreased and that of p-IKKα/β, p-p65, and CRP significantly increased in the mimic group, and the opposite was true in the inhibitor group. Conclusions:In paediatric MPP, increased miR-10a-3p downregulated COX11, activating NF-κB signalling pathway to promote disease development and progression.Li et al. miR-10a-3p targets COX11 leading paediatric MPP progression

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Significance changes in the levels of myocardial enzyme in the child patients with Mycoplasma Pneumoniae Pneumonia

Cited by 6 publications

References 18 publications

Epidemiologic and clinical characteristics of human bocavirus infection in infants and young children suffering with community acquired pneumonia in Ningxia, China

Epidemiologic and clinical characteristics of human bocavirus infection in infants and young children suffering with community acquired pneumonia in Ningxia, China

Serum amyloid a, C‐reactive protein, and procalcitonin levels in children with Mycoplasma pneumoniae infection

The role of targeted regulation of COX11 by miR-10a-3p in the development and progression of paediatric mycoplasma pneumoniae pneumonia

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