Significance of Adenosine Deaminase Serum Concentrations in the Diagnosis of Extra-Pulmonary Tuberculosis

Stevanović, Goran

doi:10.5272/jimab.2011171.130

Cited by 9 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The commonest pattern in this study was pattern B (66.34% cases) showing epithelioid granuloma with necrosis. The same was observed by Bhattacharya et al[18] and Khanna et al[19] in 69.4% and 50.5% cases, respectively.Results similar to our study were also demonstrated in the studies by Sulakshana et al[21], Ahmed et al[22], Abdelsadek et al[23], Mugulkod et al[15], and Stevanovic et al[3]. This was in contrast to the findings of Conde et al[22].…”

supporting

confidence: 92%

“…In 1993, TB was declared as a public health emergency on a global level by WHO [2]. Depending upon the locus of involvement, TB can have two different manifestations clinically: Pulmonary TB, wherein infection is situated in the lungs and Extrapulmonary TB, involving any site other than the lungs, for example, abdomen, lymph nodes, meninges, skin, or genito-urinary tract [3]. The most common manifestation of mycobacterial extra-pulmonary disease is the involvement of peripheral lymph nodes, usually of the cervical area [4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Association of Serum Adenosine Deaminase Levels in Cytologically Suggested Cases of Tubercular Lymphadenitis: The Experience of a Tertiary Care Centre

Garg

Arora

Joshi

et al. 2021

SJMS

View full text Add to dashboard Cite

Background: Tuberculosis (TB), a communicable disease, caused by Mycobacterium tuberculosis requires a simple, rapid test, which can be easily carried out in a laboratory. Unfortunately, despite a battery of investigations, no definite test is available till date. Adenosine deaminase (ADA), a biochemical marker has been proposed as a useful surrogate marker for TB as its levels can be measured in body fluids. Methods: A one-and-a-half-year prospective study of 154 cases presenting with lymphadenitis from January 2019 to June 2020 was undertaken. Using cytology, lymphadenitis subjects were divided into two groups: Tubercular (104 patients) as a case group and Reactive (50 patients) as a control group. All cases were followed by serum ADA assay by colorimetric method. Nonparametric tests were performed to compare the two groups. Results: The mean age of the participants was 28.99 ± 13.26 years with a F:M ratio of 1.81:1. Involvement of cervical lymph nodes was most frequent (89.42% cases). The mean S.ADA level for tubercular and reactive lymphadenitis was 41.71 ± 11.53 U/L and 21.16 ± 4.16 U/L, respectively (P-value < 0.05). The cut-off value calculated was 32.6 U/L. The sensitivity, specificity, PPV, NPV, and accuracy were calculated as 79.81%, 100%, 100%, 70.42%, and 86.36%, respectively. Conclusion: A statistically significant increase was found in serum ADA levels in tubercular lymphadenitis cases compared to reactive lymphadenitis. Hence, it can be used as an adjunct to FNAC and is a fairly sensitive and specific test. Since it is difficult to always demonstrate AFB in FNAC smears, ADA can be helpful in establishing a definite diagnosis despite smear negativity. Keywords: adenosine deaminase, lymphadenitis, tuberculosis

show abstract

supporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Association of Serum Adenosine Deaminase Levels in Cytologically Suggested Cases of Tubercular Lymphadenitis: The Experience of a Tertiary Care Centre

Garg

Arora

Joshi

et al. 2021

SJMS

View full text Add to dashboard Cite

show abstract

“…Activity of this enzyme increases in TB patients. In some studies, the level of ADA in sputum and serum was used for diagnosis of TB, and it was monitored during TB treatment (Afrasiabian et al,2013;Stevanovic et al, 2011).…”

Section: Management Of Chylous Ascitesmentioning

confidence: 99%

“…Accordingly, there develops tests based on biochemical response of body towards TB infection. Adenosine deaminase (ADA) test is one of biochemical test for TB (Stevanovic et al, 2011;Ninghot et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and management problems of chylous effusion in a patient with newly-diagnosed tuberculosis

Febrianto¹,

Hadi²

2021

CIMRJ

View full text Add to dashboard Cite

Tuberculosis (TB) remains a major cause of morbidity and mortality globally. Although TB most commonly affects the lungs, any organ or tissue can be involved. Extra-pulmonary forms of TB are commonly unrecognized or late diagnosed. Chylous effusion, i.e. chylothorax and chylous ascites, which is characterized by the presence of chyle in the pleural and peritoneal cavities, is an uncommon manifestation of extra-pulmonary TB. A 22-year-old male, referred to Dr. Soetomo Hospital with complaints of dyspnea, fever, and abdominal distension. Chest X-ray showed pleural effusion. Analysis of fluid obtained from thoracentesis and paracentesis showed chylothorax and chylous ascites. M. tuberculosis had been found in sputum smear examination. ADA (adenosine deaminase) test was performed on ascites fluid and a positive result was obtained. Chylous effusion in this patient were concluded to be related to TB. Patient was then treated with anti-TB drugs and somatostatin. Chylothorax and chylous ascites improved after treatment with somatostatin for 1 week. Administration of anti-TB drugs was planned to be continued for 9 months. The most common causes of non-traumatic chylous effusion in developing countries are infection of TB and filariasis. Chylous effusion is caused by obstruction or disruption of the lymphatic system. ADA test is a new biomedical method that begins to expand its use in body fluids to diagnose extra-pulmonary TB. Fasting, together with total parenteral nutrition, can decrease the lymph flow and balance metabolic impairment. Somatostatin has been used in the treatment of chylous effusion as it diminishes peristalsis and intestinal absorption of fats as well as decreases portal pressure.

show abstract

“…The previous studies have proved its value in TB diagnosis, even for assessing TB effusions in which activity of this enzyme increases in the infected patients 7 .In some studies, the level of ADA in body fluids and serum was used for diagnosis of TB and it was monitored during TB treatment 8 . Initial studies used effusion fluids and a very limited number of studies used patients' serum 9 . Since, it is not always possible to access effusion liquids everywhere in pulmonary and extra pulmonary TB; therefore, it would be helpful to take advantage of serum levels.…”

mentioning

confidence: 99%

Serum Adenosine Deaminase: A Novel Biomarker Tool for the Diagnosis of Tuberculosis

Pandey¹,

Tamrakar²,

Jaiswal³

et al. 2016

Biosci., Biotech. Res. Asia

View full text Add to dashboard Cite

Tuberculosis is a major health issues worldwide. Every year more than 9 million new cases are reported worldwide with death rate of around 2 million each year. Rapid and accurate diagnosis of tuberculosis in population is most supportive in proper medication and early recovery. The golden standard method for diagnosis of tuberculosis in patient is formerly culture method which is time consuming and cumbersome. Various other techniques used in the diagnosis of tuberculosis have poor sensitivity and specificity however serum adenosine deaminase (ADA) has emerged as a potent biochemical marker that can be used as tool for the diagnosis of tuberculosis for rapid, easy and better result. Increased level of ADA in blood generally indicates the presence of tuberculosis. In the present study, the serum ADA level was examined in 75 healthy control and 75 tuberculosis patients with positive sputum smear for acid fast bacilli (AFB), having clinical symptoms were diagnosed for pulmonary tuberculosis and radiological impression for extra pulmonary tuberculosis. The selection of cases and control were based on the inclusion and exclusion criteria. Samples were collected at the outpatient department of National Tuberculosis Centre, Nepal after asking simple questionnaires. Modified Guisti and Galanti method was adopted for estimation of serum ADA from pooled and processed blood samples. There was significant difference (p<0.001) in mean rank of level of serum ADA among the TB cases to controls. The P-TB had the highest mean rank (116.52) followed by EP-TB (99.48) and control had significantly less mean rank (40.16). At the cut-off point of 25 U/L; sensitivity, specificity, positive predictive value and negative predictive value were 90.7%, 100%, 90.66% and 100% respectively. It can be concluded from data that there were insignificant difference in mean rank among sex wise distribution with p=0.037 and sputum grading wise distribution with p=0.142.

show abstract

Significance of Adenosine Deaminase Serum Concentrations in the Diagnosis of Extra-Pulmonary Tuberculosis

Cited by 9 publications

References 14 publications

Association of Serum Adenosine Deaminase Levels in Cytologically Suggested Cases of Tubercular Lymphadenitis: The Experience of a Tertiary Care Centre

Association of Serum Adenosine Deaminase Levels in Cytologically Suggested Cases of Tubercular Lymphadenitis: The Experience of a Tertiary Care Centre

Diagnostic and management problems of chylous effusion in a patient with newly-diagnosed tuberculosis

Serum Adenosine Deaminase: A Novel Biomarker Tool for the Diagnosis of Tuberculosis

Contact Info

Product

Resources

About