Significance of ADP, Plasma and Platelet Concentration in Platelet Electrophoretic Studies

Betts, J. J.; Betts, J.P.; Nicholson, J. T.

doi:10.1038/2191280b0

Cited by 15 publications

(8 citation statements)

References 11 publications

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“…Recent reports have characterised ADPinduced mobility changes and have specified factors which influence both these changes and ADPinduced aggregation. The electrophoretic mobility changes induced by ADP are biphasic in character (Betts, Betts & Nicholson, 1968;Hampton & Mitchell, 1966b); low concentrations of ADP (-0-05 ,ug/ml) increase platelet mobility and high concentrations (> 0 5 ,ug/ml) decrease mobility. Hampton & Mitchell (1966a) reported that in acute pathological states, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…myocardial infarction, platelets show increased sensitivity to ADP and concentrations much less than 0 05 Fg/ml induce mobility increases. Bolton, Hampton & Mitchell (1967) showed that abnormal plasma phospholipid levels could sensitize platelets to ADP and Betts et al (1968) showed that ADP-induced mobility increases are influenced by the presence of fibrinogen.…”

Section: Discussionmentioning

confidence: 99%

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Acanthocytosis with normolipoproteinaemia: biophysical aspects

Betts¹,

Nicholson²,

Critchley³

1970

Postgraduate Medical Journal

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SummaryThe electrokinetic properties of normal and pathological red cells and platelets are compared.The electrophoretic mobilities and isoelectric points of normal and pathological erythrocytes do not differ significantly, adsorption of calcium and uranyl ions occur to the same extent and treatment with neuraminidase induces almost identical mobility decreases. The pathological platelet-plasma system is more sensitive to adenosine diphosphate (ADP) than normal systems; platelet aggregation and electrophoretic mobility increases are induced by much lower concentrations of ADP than those normally required. The pathological platelet system shows a greater tendency to aggregate spontaneously. The abnormal sensitivity to ADP of the pathological platelet-plasma system is considered in terms of plasma components.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Acanthocytosis with normolipoproteinaemia: biophysical aspects

Betts¹,

Nicholson²,

Critchley³

1970

Postgraduate Medical Journal

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“…The technique is also applicable to laboratory animals; the biphasic electrophoretic mobility response to aggregating agents was first demonstrated in pigs (Betts, Betts & Nicholson, 1968). Using the horizontal cylindrical apparatus (Bangham, Flemans, Heard & Seaman, 1958), but with solid silver- silver chloride electrodes, I have found that a small chamber with a capacity of 1 ml can be used to make observations on platelets from animals with small blood volumes.…”

Section: Electrophoretic Mobilitymentioning

confidence: 99%

The importance of platelet function tests in toxicological screening using laboratory animals

Hawkins

1972

Lab Anim

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The haemostatic mechanism depends partly upon platelet activity. An increase or decrease in this activity will result in clotting or bleeding abnormalities respectively. Changes of platelet function suggest that an haemorrhagic or thrombo-embolic condition may result from the altered haemostasis induced, for example, by drug or dietary action. It is important therefore that assessment of platelet function is included in normal toxicity screening. In order for this to be carried out it has been established that these tests are applicable to the laboratory animals used for this procedure, and examples given of the typical results obtained. DIE BEDEUTUNG YON BLUTPLATTCHEN .FUNKTION TESTS IN TOXIKOLOGlSCHEN BERECHNUNGEN IN DENEN LABORTlERE BENUTZT WERDEN Der hamostatische Mechanismus hangt zurn Teil von der Blutplattchen Aktivitat abo Eine Erhohung oder Verminderung in dieser Aktivitat bringt Abnormalitaten von Gerinnung resp. B1utungen hervor. Anderungen in der Funktion derBiutplattchen lassen annehmen, dass ein hamorrhagischer oder thrombo-embolischer Zustand durch geanderte induzierte Hamostasis, z.B. durch Drogen oder Diatseingriffe, entstehen kann. Es ist daher wichtig, dass in normalen ToxiziUitsberechnungen die Messung der Blutpliittchen Funktion eingefUgt wird. Damit das gemacht wird, zeigt man dass diese Tests auf die Labortiere, die in diesen Versuchen benutzt werden, anwendbar sind, und es werden Beispiele von typischen Resultaten gegeben. L'IMPORTANCE DES TESTS SUR LA FONCTlON DES HEMATOBLASTES DANS L'EYALUATION TOXICOLOGIQUE UTlLlSANT DES ANIMAUX DE LABORATOIRELe mecanisme hemostatique depend en mesure de l'activite des hematoblastes. Dne augmentation ou diminution dans cette activite resultera en abnormalites soit de coagulation soit d'hemorrhagie. Des changements dans la fonction des hematoblastes suggerent qu'une condition hemorrhagique ou thrombo-embolique peut resulter, par exemple, d'une hemostasie induite

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“…Since the ADP molecule does not penetrate the platelet membrane but produces highly specific processes, the ADP-fibrinogen-platelet reactions most likely take place at the platelet surface (30). It has been suggested that the fibrinogen (3) and yM-globulin adsorbed on to platelets (18) play an essential part in certain reactions (3,18,34), including the interaction of ADP with the platelet membrane. Let us consider the role of the surface molecular complex in platelet aggregation caused by anionic polymers and other charged clumping agents (12,20,36), particularly by ADP (e.g.…”

Section: Cell Siirface Groups/receptors? In Platelet Aggregation and mentioning

confidence: 99%

The Human Blood Platelets as a Molecular Mosaic Its Role in Aggregation and Thrombus Formation

Mehrishi¹

1971

Thromb Haemost

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SummaryOn the surface of human blood platelets various types of groups are present - sulphydryl groups, positively charged amino groups and at least three types of anionogenic groups. The numbers of these groups on a per cell basis have been calculated and then* relative contributions to the net charge determined. Two arrangements for the distribution of the groups in the cell periphery have been proposed. Such studies provide a molecular basis for many reactions taking place at the cell surface. The role of these groups in platelet aggregation and thrombus formation is discussed

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Significance of ADP, Plasma and Platelet Concentration in Platelet Electrophoretic Studies

Cited by 15 publications

References 11 publications

Acanthocytosis with normolipoproteinaemia: biophysical aspects

Acanthocytosis with normolipoproteinaemia: biophysical aspects

The importance of platelet function tests in toxicological screening using laboratory animals

The Human Blood Platelets as a Molecular Mosaic Its Role in Aggregation and Thrombus Formation

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