Significance of AgNOR Count in Differentiating Malignant Cells from Reactive Mesothelial Cells in Serous Effusions

Sujathan, K.; Kannan, S.; Pillai, Kamala; Chandralekha, B.; Amma, N. Sreedevi; Nair, Muralidharan

doi:10.1159/000333946

Cited by 25 publications

(25 citation statements)

References 22 publications

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“…38,39 The size of the labeled areas in benign and malignant cells, respectively, in our series of effusions is in good agreement with these observations. Thus, they are also in agreement with the conclusions drawn by Wu et al 37 regarding the nature of the reactivity of NCL-hTERT.…”

Section: Discussionsupporting

confidence: 90%

Comparison of NCL-hTERT Antibody Reactivity and Telomere Repeat Amplification Protocol in Situ in Effusions

Zendehrokh

Rehnberg²,

Dejmek

2007

Acta Cytologica

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Section: Discussionsupporting

confidence: 90%

Comparison of NCL-hTERT Antibody Reactivity and Telomere Repeat Amplification Protocol in Situ in Effusions

Zendehrokh

Rehnberg²,

Dejmek

2007

Acta Cytologica

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“…21,22 So AgNOR count appears as an important additional diagnostic tool for use in ascitic and pleural fluid samples when routine cytological diagnosis is difficult. 23 Other co-workers have also documented that AgNOR count is higher in malignant than in benign mesothelial cells. 24 Following the criteria devised by Ahsan et al in the present study, it was found that in addition to the high number of AgNOR counts, AgNOR distribution was irregular and had a grade of 2+ to 3+ in the majority of the malignant effusions.…”

Section: Discussionmentioning

confidence: 94%

The Role of Argyrophilic Nucleolar Organizer Region (AgNOR) Study in Cytological Evaluation of Fluids, Especially for Detection of Malignancy

Karki¹,

Jha²,

Sayami³

2012

Kathmandu Univ. Med. J.

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BackgroundSerous effusion smears reported as "suspicious for malignancy" pose problems in clinical management. Silver staining for argyrophilic nucleolar organizer regions (AgNOR) has proved useful in making a cytopathologic differential diagnosis between benign and malignant cells. Nucleolar organizer regions(NORs) are loops of DNA located in acrocentric chromosomes. These NORs are visualized by silver staining technique that recognizes these argyrophilia associated proteins which are increased in malignancy. ObjectiveThis study aimed to distinguish reactive mesothelial cells from malignant cells in serous effusions using these NORs. MethodsA total of 174 serous effusions received at the Department of Pathology, TUTH, during a period of one year were included in the study. Smears were studied by conventional Papanicolaou and Giemsa stains. AgNOR counts, variation in size and dispersion of AgNOR dots in smears were graded and compared in malignant and non-malignant effusions. ResultsMean AgNOR counts of 10.43±0.73 and 10.21±0.51 in malignant peritoneal and pleural effusions, respectively, were significantly (p<0.0001) greater as compared with counts of 2.12±0.54 and 2.11±0.54 in non-malignant effusions. The AgNORs were irregular in shape in malignant effusions whereas they were comparatively larger, single dots in benign effusions. AgNOR size and dispersion were of higher grade in significantly greater proportion of malignant as compared with non malignant effusions (p<0.0001). Of the cytologically suspicious samples, nine were in the malignant range and one was in the benign range. ConclusionAgNOR study appears to be clinically useful as an additional diagnostic tool for use in serous effusion when the cytologic diagnosis is difficult.

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“…It has been demonstrated that the number of AgNORs per nucleus can be regarded as one of the hallmarks of proliferation and that mean AgNOR counts are good indicators of the degree of malignancy of human non-Hodgkin's lymphoma. 2,10,11,20,24,28,33,34 Moreover, AgNOR counts were also found to be indicators of postsurgical prognosis in canine mast cell tumors. 4,22 The results obtained by various research groups consistently show that a rising AgNOR count is indicative of an increasing tendency toward proliferation; however, there are differences between the values obtained.…”

Section: Introductionmentioning

confidence: 99%

Use of the Argyrophilic Nucleolar Region Method for Cytologic and Histologic Examination of the Lymph Nodes in Dogs

et al. 2004

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Abstract. Argyrophilic nucleolar organizer region (AgNOR) counts in cytologic and histologic lymph node samples from healthy dogs and dogs with lymphoma were compared. Control samples were taken from 10 Beagle dogs (six female and four male dogs, 1.5-2 years), and lymphoma samples were taken from 16 dogs. Cytologic samples were obtained by fine-needle aspiration and impression and histologic samples by excision or incisional biopsy. Altogether, 26 cytologic, 19 excisional, and 7 incisional biopsy samples were examined. Lymph nodes of controls showed a moderate inflammatory pattern; of the lymphoma cases, nine were lowgrade forms and seven were high-grade forms. Mean AgNOR counts per nucleus were determined. AgNOR counts were statistically different (P Ͻ 0.001) between controls and lymphoma cases in cytologic (1.35 and 3.59, respectively) and histologic (1.4 and 2.89, respectively) samples. In lymphoma cases, AgNOR counts in cytologic samples were higher than those in histologic samples by 0.81 (P Ͻ 0.001) and showed a linear relationship (r ϭ 0.6; P Ͻ 0.05) with the histologic counterparts in excisional biopsy samples. AgNOR counts in low-and high-grade lymphomas were significantly different (P Ͻ 0.05) in cytologic (3.21 and 4.08, respectively) and histologic (2.68 and 3.18, respectively) samples. In conclusion, AgNOR counts were higher in lymph nodes with lymphoma than in reactive nodes. In the case of dogs with lymphoma, AgNOR counts in cytologic samples were linearly related to excisional but not to incisional biopsy samples. Although AgNOR counts were different between cytologic and histologic samples, either sample type provided enough sensitivity to differentiate between high-and low-grade forms of lymphoma.

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Significance of AgNOR Count in Differentiating Malignant Cells from Reactive Mesothelial Cells in Serous Effusions

Cited by 25 publications

References 22 publications

Comparison of NCL-hTERT Antibody Reactivity and Telomere Repeat Amplification Protocol in Situ in Effusions

Comparison of NCL-hTERT Antibody Reactivity and Telomere Repeat Amplification Protocol in Situ in Effusions

The Role of Argyrophilic Nucleolar Organizer Region (AgNOR) Study in Cytological Evaluation of Fluids, Especially for Detection of Malignancy

Use of the Argyrophilic Nucleolar Region Method for Cytologic and Histologic Examination of the Lymph Nodes in Dogs

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