Significance of antiprothrombin antibodies in patients with systemic lupus erythematosus: clinical evaluation of the antiprothrombin assay and the antiphosphatidylserine/prothrombin assay, and comparison with other antiphospholipid antibody assays

Tsutsumi, Akito; Hayashi, Taichi; Chino, Yusuke; Mamura, Mizuko; Goto, Daisuke; Matsumoto, Isao; Ito, Satoshi; Sumida, Takayuki

doi:10.1007/s10165-006-0481-7

Cited by 23 publications

(24 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5,7 Several studies have been published with regard to the relationship between the presence of aPT-A and APS-related clinical features with conflicting conclusions. [8][9][10][11][12][13][14][15][16][17][18][19][20] A recent systematic review suggested that both antibodies against prothrombin, aPT-A and aPS/PT, are risk factors for thrombosis, but that aPS/PT represent a stronger risk factor for arterial and/or venous thrombosis when compared to aPT-A. 21 In two prospective studies, the presence of aPT-A has been reported as a predictor of thromboembolic events in patients with aPL, mainly in those patients positive for LA.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study

Amengual

Forastiero

Sugiura‐Ogasawara

et al. 2016

Lupus

View full text Add to dashboard Cite

Objective: A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/ PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. Methods: In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. Results: In the initial study (n ¼ 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k ¼ 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LRþ) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n ¼ 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k ¼ 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR þ and LR-for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. Conclusions: IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS. Lupus (2016) 0, 1-11.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: results of an international multicentre study

Amengual

Forastiero

Sugiura‐Ogasawara

et al. 2016

Lupus

View full text Add to dashboard Cite

show abstract

“…Those autoantibodies are strong risk factors for venous thromboembolism in patients with SLE because they induce activated protein C resistance [37]. The other two IgG autoantibodies, namely antithrombin and aPL, are also associated with thrombotic events in aPL syndrome and SLE [32,33,50,51], and are risk factors for myocardial infarction in middle-aged men [52,53]. …”

Section: Discussionmentioning

confidence: 99%

The thrombophilic network of autoantibodies in celiac disease

Lerner

Agmon‐Levin

Shapira

et al. 2013

BMC Med

View full text Add to dashboard Cite

BackgroundCeliac disease is a life-long autoimmune condition, affecting genetically susceptible individuals that may present with thromboembolic phenomena. This thrombophilia represents a puzzle with multiple constituents: hyperhomocysteinemia, B12 and\or folate deficiency, methylenetetrahydrofolate reductase mutations, and protein C and S deficiency due to vitamin K deficiency. However, the well known thrombogenic factors, antiphosphatidylserine/prothrombin and antiprothrombin have never been explored in celiac disease.MethodsThe serum autoantibody levels were determined in 248 individuals, classified into three groups. Group 1 comprised 70 children with definitive celiac disease (age: 7.04 ±4.3 years, male to female ratio 1.06) and group 2 comprised 88 normal children (age: 6.7 ±4.17 years, male to female ratio 0.87), representing controls. The pediatric populations were compared to group 3, which included 90 adults who were family members (parents) of group 1 (age: 34.6 ±11.35 years, male to female ratio 1.2). Antibodies were checked by enzyme-linked immunosorbent assay.ResultsMean optical density levels of serum antiphosphatidylserine/prothrombin immunoglobulin G antibodies were 32.4 ±19.4, 3.6 ±2.5 and 16.1 ±15.8 absorbance units in groups 1, 2 and 3 respectively (P <0.0001), with 45.7%, 0% and 7.8% of groups 1, 2 and 3 respectively positive for the antibody (P <0.01). Mean optical density levels of serum antiphosphatidylserine/prothrombin immunoglobulin M antibodies were 14.2 ±8.7, 6.7 ±6.4 and 12.4 ±15.5 absorbance units in groups 1, 2 and 3 respectively (P <0.0001), with 7.1%, 3.4% and 9.9% of groups 1, 2 and 3 positive for the antibody. Mean optical density levels of serum antiprothrombin and antiphospholipid immunoglobulin G antibodies were higher in groups 1 and 3 compared with 2 (P <0.005) and in groups 1 and 2 compared with 3 (P <0.01), respectively. Groups 1, 2 and 3 were positive for antiphospholipid immunoglobulin G antibodies (groups 1 and 2 compared with 3) . Celiac disease sera harbor a higher antiprothrombin immunoglobulin G level compared with controls.ConclusionsIt is suggested that the intestinal injury, endothelial dysfunction, platelet abnormality and enhanced apoptosis recently described in celiac disease are at the origin of the increased exposure of phospholipids or new epitopes representing autoantigens. Those autoantibodies might play a pathogenic role in the thrombophilia associated with celiac disease and represent markers for potential anticoagulant preventive therapy.

show abstract

“…Antiphospholipid antibodies are known to bind to plasma proteins with an affinity for phospholipids' surfaces [22][23][24][25]. Prothrombin is another important autoantigen recognized by anti-phospholipid antibodies [26,27]. The role of the anti-prothrombin (aPT) antibodies in the development of thrombosis has been evaluated aPT IgG results are expressed in units relative to the calibrator, Arbitrary Unit (AU) /ml, as follows: negative < 10 AU/ml, 'grey zone' 10-20 AU/ml, positive ≥20 AU/ml.…”

Section: Discussionmentioning

confidence: 99%