Significance of Asaccharolytic Eubacterium and Closely Related Bacterial Species in the Human Oral Cavity

Nakazawa, Fumio; Miyakawa, Hiroshi; Fujita, Mari; Kamaguchi, Arihide

doi:10.1016/j.jecm.2010.12.008

Cited by 5 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Streptococcus anginosus group comprised of three species, S. anginosus , S. constellatus and S. intermedius and are normal flora in humans, these bacteria are pathogens associated strongly with abscess formation and with infection in multiple body sites [75]. Assacharolytic Eubacterium and closely related strains found in our study at tumor sites are major bacterial groups in oral lesions and play important role in infections of root canal and periodontal pockets and use proteins and peptides derived from tissues and blood as energy source [76]. Also, Atopobium , F. nucleatum ss.…”

Section: Discussionmentioning

confidence: 99%

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

et al. 2012

View full text Add to dashboard Cite

BackgroundBacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples.ResultsDGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp. oral taxon 058, Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites.ConclusionsThe underlying changes in the bacterial diversity in the oral mucosal tissues from non-tumor and tumor sites of OSCC subjects indicated a shift in bacterial colonization. These most prevalent or unique bacterial species/phylotypes present in tumor tissues may be associated with OSCC and needs to be further investigated with a larger sample size.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

et al. 2012

View full text Add to dashboard Cite

show abstract

“…S. exigua have been significantly associated with periodontitis as observations revealed higher antibody titers in the serums of periodontitis patients. [ 25 ] Serum IgA levels against S. exigua were elevated in cases of refractory periodontitis, IgG levels in chronic periodontitis patients suggests that these species have breached the host defense to stimulate an immune response. S. exigua produced butyric acid from arginine that has a key role in promoting halitosis.…”

Section: R Eview Of P Hylogeny mentioning

confidence: 99%

“…Butyric acid also inhibits the proliferation of gingival fibroblasts and induces apoptosis in splenic T cells further leading to exacerbation of infectious lesions. Uematsu and Hoshino isolated strains from periodontal pockets of chronic periodontitis patients, of which 42% were Eubacteria species[ 25 ] and many studies have reported that S. exigua plays a pivotal role in moderately and severely affected periodontal disease as it is frequently isolated in periodontal lesions. S. exigua is susceptible to antimicrobials but resistant to sulfamethaxazole – trimethoprim.…”

Section: R Eview Of P Hylogeny mentioning

confidence: 99%

See 1 more Smart Citation

Novel pathogens in periodontal microbiology

et al. 2017

View full text Add to dashboard Cite

Periodontitis is a polymicrobial disease caused by complex interactions between distinct pathogens in a biofilm resulting in the destruction of periodontal tissues. It seems evident that unknown microorganisms might be involved in onset or progression of periodontitis. For many decades, research in the field of oral microbiology failed to identify certain subgingival microbiota due to technical limitations but, over a period of 12 years using molecular approaches and sequencing techniques, it became feasible to reveal the existence of new periodontal pathogens. Therefore, it is evident that in addition to conventional periodontal pathogens, other microbes might be involved in onset and progression of periodontitis. The novel pathogens enlisted under periodontal phylogeny include Cryptobacterium curtum, Dialister pneumosintes, Filifactor alocis, Mitsuokella dentalis, Slackia exigua, Selenomonas sputigena, Solobacterium moorei, Treponema lecithinolyticum, and Synergistes. The polymicrobial etiology of periodontitis has been elucidated by comprehensive techniques, and studies throwing light on the possible virulence mechanisms possessed by these novel periodontal pathogens are enlisted.

show abstract

“…Streptococcus was also found in some of the studies analysing human dental aerosols [8,12]. E. lenta has only been found in humans as being part of the intestinal microbiome, and it has also been found as one of the microorganisms forming part of the subgingival flora in patients with periodontal disease [18]. R. pickettii has been identified in cases of human medicine infections due to contaminated solutions, as water for injections, saline solutions, and sterile drug solutions [19].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Aerosols Released During Dental Ultrasonic Scaling in Dogs

Delgado¹,

Dias²

2020

DOBCR

View full text Add to dashboard Cite

Periodontal disease is a high prevalent and multi-factorial oral disease in dogs and ultrasonic scaling is used to remove dental plaque and calculus, releasing contaminated aerosols, which may represent a hazard to animal and human health. This study aimed to identify the microorganisms present in aerosols produced during dental scaling of canine patients. A random sample of 15 dogs with periodontal disease was included, and aerosol samples were collected, incubated, and isolated bacteria were identified. Dogs without previous antibiotic treatment (n=4) and dogs that received systemic antibiotics up to two weeks before to dental scaling and polishing, either amoxicillin and clavulanic acid (n=3), or a combination of metronidazole and spiramycin (n=8), were included in the study. The highest percentage of pathogenic bacteria present corresponded to Pseudomonas spp. (20%), followed by Staphylococcus spp. (13%) and Escherichia coli (10%). The most prevalent bacteria identified in the non-treated group was Pseudomonas spp. Within the group subject to prior antibiotherapy, the predominant bacterial species was also Pseudomonas spp. Followed by Staphylococcus spp. In spite of previous antibiotherapy, strong bacterial contamination was still present, suggesting that this is not a warranty of less contamination of the released aerosols. The bacteria identified in this study represent a serious hazard to public and animal health, so strict hygiene and prevention measures during ultrasonic scaling in dogs are mandatory.

show abstract

Significance of Asaccharolytic Eubacterium and Closely Related Bacterial Species in the Human Oral Cavity

Cited by 5 publications

References 42 publications

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

Novel pathogens in periodontal microbiology

Bacterial Aerosols Released During Dental Ultrasonic Scaling in Dogs

Contact Info

Product

Resources

About