Significance of bacterial variants in urine of patients with chronic bacteriuria.

Gutman, Laura T.; Turck, Marvin; Petersdorf, Robert G.; Wedgwood, Ralph

doi:10.1172/jci105300

Cited by 99 publications

(41 citation statements)

References 14 publications

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“…Data in Fig. 1 show the mean values obtained in three experiments to measure the antibiotic concentrations obtained in the blood and peritoneal cavities of mice infected with E. coli 8 blood at both dose levels, 12.5 and 3.1 mg/kg, and in the peritoneal fluid at the 3.1-mg/kg dose level. In the blood, the dose of 12.5 mg/kg produced a peak level of 8.5 ,ug/ml at 10 min, which fell rapidly to undetectable levels (<0.01 ,ug/ml) by 120 min; at 3.1 mg/kg, the concentrations also fell rapidly from the peak value of 1.5 ug/ml measured at 10 min.…”

Section: Resultsmentioning

confidence: 99%

“…The morphological effects produced by benzylpenicillin on susceptible bacteria were observed soon after the antibiotic was isolated (5), and the presence of abnormal varieties, in animals or patients, after penicillin therapy has been described (2,4,8,9,12). More recently, there has been a resurgence of interest in the various morphological effects produced by 8-lactam antibiotics as a result of awareness of the variety of effects caused by compounds of differing structures (4,6,14,17), and differences in effects on morphology have been correlated with differences in affinity for binding sites (7,13,16).…”

Section: Resultsmentioning

confidence: 99%

“…Male 8 and were treated with a single subcutaneous dose of amoxycillin or ampicillin. The animals were killed at intervals after administration of the penicillins, and specimens of blood and peritoneal fluid were collected for microscopic examination.…”

Section: Methodsmentioning

confidence: 99%

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Comparative Effects of Amoxycillin and Ampicillin on the Morphology of Escherichia coli In Vivo and Correlation with Activity

Comber

Boon

Sutherland

1977

Antimicrob Agents Chemother

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An experimental mouse intraperitoneal infection due to Escherichia coli was treated with subcutaneous amoxycillin or ampicillin. Specimens of blood and peritoneal washings from the infected animals were assayed for antibiotic concentrations and examined microscopically for observation of the effects produced by the two penicillins on the morphology of bacteria growing in vivo. Amoxycillin was significantly more effective than ampicillin in protecting the animals from the lethal effects of the infection, altbough the antibiotic concentrations in the body fluids were very similar for both compounds. However, microscopic examination showed marked differences in the morphological effects produced at equivalent dose levels by the two compounds against the bacteria present in blood and peritoneal fluid. Treatment with amoxycillin at dose levels that produced peak antibiotic concentrations in the body fluids ranging from one-quarter to three times the minimum inhibitory concentration resulted in the formation of spheroplast forms, which lysed rapidly. In contrast, at the same concentrations, ampicillin produced relatively stable filaments or long cell forms, which lysed much more slowly, although at higher dose levels the effects produced were generally similar to those seen with amoxycillin. It is concluded that the superior therapeutic activity of amoxycillin compared with ampicillin is due to its greater bacteriolytic activity in vivo.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Effects of Amoxycillin and Ampicillin on the Morphology of Escherichia coli In Vivo and Correlation with Activity

Comber

Boon

Sutherland

1977

Antimicrob Agents Chemother

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“…Almost 50 years ago, Gutman, Turck, Petersdorf, and Wedgwood reported in the JCI the survival of bacterial variants in antibiotic-treated patients with pyelonephritis (20). They used painstaking methods in a pre-PCR microbiology lab for "separation of bacterial variants from classical organisms."…”

Section: Figurementioning

confidence: 99%

Remains of infection

Barbour¹

2012

J. Clin. Invest.

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“…123,124 A prospective cohort study of 57 patients with pyelonephritis or chronic bacteriuria found that 17% had CWDB in the urine, but the study lacked the statistical power to conclude that CWDB caused urinary tract infections. 125 …”

Section: Urinary Tract Infectionsmentioning

confidence: 99%

Cell Wall-Deficient Bacteria as a Cause of Infections: A Review of the Clinical Significance

2005

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Cell wall-deficient bacteria (CWDB) are pleomorphic bacterial forms. These atypical organisms may occur naturally or they can be induced in the laboratory. Their presence has been known about for over a century, but a definite link to clinical disease outcomes has not been demonstrated. A number of case reports and laboratory studies suggest some disease associations, however. Considerable controversy surrounds the true relevance of CWDB to disease; there is a widespread belief that they may represent a response by the walled organism to adverse extracellular conditions like antibiotic pressure. This review looks at studies published between 1934 and 2003, which were identified by Dialog DataStar using the key words 'cell wall deficient bacteria and clinical significance and infections' and by further scanning the reference list at the end of the papers retrieved. We conclude that the evidence for the clinical significance of CWDB in disease is not compelling.

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Significance of bacterial variants in urine of patients with chronic bacteriuria.

Cited by 99 publications

References 14 publications

Comparative Effects of Amoxycillin and Ampicillin on the Morphology of Escherichia coli In Vivo and Correlation with Activity

Comparative Effects of Amoxycillin and Ampicillin on the Morphology of Escherichia coli In Vivo and Correlation with Activity

Remains of infection

Cell Wall-Deficient Bacteria as a Cause of Infections: A Review of the Clinical Significance

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