Significance of catechol-O-methyltransferase gene polymorphism in fibromyalgia syndrome

Gürsoy, Savaş; Erdal, Emin; Herken, Hasan; Madenci, Ercan; Alaşehirli, Belgin; Erdal, Nurten

doi:10.1007/s00296-002-0260-5

Cited by 234 publications

(150 citation statements)

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“…The primers described by Gursoy et al [8] were used to amplify a 185-bp fragment of the COMT gene containing the SNPs rs4680 and rs4818. The PCRs were done according to Matsuda et al [13], and the amplicons were sequenced by the dideoxy chain termination method using an automated DNA sequencer (ABI 377).…”

Section: Molecular Analysis Of the Comt Gene Polymorphismsmentioning

confidence: 99%

“…Although the physiological mechanisms that control FS have not been precisely established, neuroendocrine, genetic, and molecular mechanisms may be involved in fibromialgia [4,7]. Catechol-O-methyltransferase (COMT) is an enzyme that inactivates catecholamines and catechol-containing drugs [8]. There are different single-nucleotide polymorphisms (SNPs) in the COMT gene which induce important functional alterations of the enzyme that are associated with fibromyalgia [5,8].…”

Section: Introductionmentioning

confidence: 99%

“…Catechol-O-methyltransferase (COMT) is an enzyme that inactivates catecholamines and catechol-containing drugs [8]. There are different single-nucleotide polymorphisms (SNPs) in the COMT gene which induce important functional alterations of the enzyme that are associated with fibromyalgia [5,8]. The best studied SNP (rs4680) occurs in codon 158, resulting in a valine (Val GTG) to methionine (Met ATG) transition.…”

Section: Introductionmentioning

confidence: 99%

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Influence of catechol-O-methyltransferase (COMT) gene polymorphisms in pain sensibility of Brazilian fibromialgia patients

Barbosa

Matsuda

Mazucato³

et al. 2010

Rheumatol Int

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Fibromyalgia syndrome (FS) is a rheumatic syndrome affecting to 2-3% of individuals of productive age, mainly women. Neuroendocrine and genetic factors may play a significant role in development of the disease which is characterized by diffuse chronic pain and presence of tender points. Several studies have suggested an association between FS, especially pain sensitivity, and polymorphism of the catechol-O-methyltransferase (COMT) gene. The aim of the present study was to characterize the SNPs rs4680 and rs4818 of the COMT gene and assess its influence in pain sensitivity of patients with fibromyalgia screened by the Fibromyalgia Impact Questionnaire (FIQ). DNA was extracted from peripheral blood of 112 patients with fibromyalgia and 110 healthy individuals and was used as template in PCR for amplification of a 185-bp fragment of the COMT gene. The amplified fragment was sequenced for analyses of the SNPs rs4680 and rs4818. The frequency of mutant genotype AA of SNP rs6860 was 77.67% in patients with FS and 28.18% for the control group. For the SNP rs4818, the frequency of mutant genotype CC was 73.21 and 39.09% for patients with FS and controls, respectively. Moreover, the FIQ score was higher in patients with the homozygous mutant genotype for SNPs rs4680 (87.92 points) and rs4818 (86.14 points). These results suggest that SNPs rs4680 and rs4818 of the COMT gene may be associated with fibromyalgia and pain sensitivity in FS Brazilian patients.

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Section: Molecular Analysis Of the Comt Gene Polymorphismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of catechol-O-methyltransferase (COMT) gene polymorphisms in pain sensibility of Brazilian fibromialgia patients

Barbosa

Matsuda

Mazucato³

et al. 2010

Rheumatol Int

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“…Thirty-three studies were identified by electronic or manual searching, and twenty-two were selected for a full-text review based on title and abstract details [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][36][37][38]. Four studies were excluded because they were reviews [5,36,37] or because they did not concern fibromyalgia [38].…”

Section: Studies Included In the Meta-analysismentioning

confidence: 99%

“…Significant familial aggregation, convincing demonstrations of genetic linkages and associations demonstrate an underlying genetic basis for fibromyalgia [5]. Many studies have examined the potential contribution of the candidate gene polymorphisms to fibromyalgia susceptibility, but these studies have produced diverse results [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Candidate gene studies of fibromyalgia: a systematic review and meta-analysis

et al. 2010

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The aim of this study was to explore whether the candidate gene polymorphisms contribute to fibromyalgia susceptibility. The authors conducted a meta-analysis on associations between serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR) S/L allele, catechol-O-methltransferase (COMT) val158Met, and serotonin 2A (5-HT2A) receptor 102T/C polymorphisms and fibromyalgia susceptibility as determined using the following: (1) allele contrast, (2) recessive, (3) dominant models, and (4) contrast of homozygotes. We also performed a systematic review with available data of the candidate genes. A total of 21 separate comparisons were considered in this systematic review and meta-analysis. Seventeen candidate genes and over 35 different polymorphisms were identified in studies on fibromyalgia susceptibility. Meta-analysis of the 5-HTTLPR S/L allele and COMT val158Met failed to reveal any association with fibromyalgia. However, meta-analysis of the C allele, CC + CT genotype, and CC versus TT genotype of the 5-HT2A receptor 102T/C polymorphism showed significant association with fibromyalgia. The overall OR of the association between the C allele and fibromyalgia was 1.333 (95% CI = 1.053-1.688, P = 0.017). The ORs for the CC + CT genotype, and CC versus TT genotype showed the same pattern as that observed for the C allele (OR = 1.541, 95% CI = 1.032-2.303, P = 0.035; OR = 1.838, 95% CI = 1.151-2.936, P = 0.011). This meta-analysis demonstrates that the 5-HT2A receptor 102T/C polymorphism confers susceptibility to fibromyalgia. In contrast, no association was found between the 5-HTTLPR S/L allele, COMT val158Met, and susceptibility to fibromyalgia.

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Management of Fibromyalgia Syndrome

Goldenberg¹

2004

JAMA

739

476

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T ANY ONE TIME, 10% TO 12% of the general population report chronic generalized musculoskeletal pain that cannot be traced to a specific structural or inflammatory cause. 1 Such idiopathic widespread pain most often will fit the classification criteria for fibromyalgia syndrome (FMS). 2 The diagnosis of FMS is based on a history of widespread pain, defined as bilateral, upper and lower body, as well as spine, and the presence of excessive tenderness on applying pressure to 11 of 18 specific muscle-tendon sites. 3 The 1990 American College of Rheumatology classification criteria for the diagnosis of fibromyalgia provide a sensitivity and specificity of nearly 85% in differentiating FMS from other forms of chronic musculoskeletal pain. Surveys using these criteria have found an FMS prevalence of 2% in the United States, including 3.4% of women and 0.5% of men. 4,5 Fibromyalgia is the second most common disorder observed by rheumatologists (after osteoarthritis), yet rheumatologists in the United States currently provide care for less than 20% of individuals with fibromyalgia. 6,7 Chronic pain syndromes, such as FMS, are defined by subjective symptoms and lack unique pathophysiological characteristics. Questions often arise regarding the nature and existence of illnesses like FMS. Indeed, no discrete boundary separates syndromes such as FMS, chronic fatigue syndrome, irritable bowel syndrome, or chronic muscular headaches. 8 Furthermore, these illnesses are each comorbid with mood disturbances. 9 Defining pain syndromes like FMS, headaches, or back pain provides a common framework to study the clinical and physiological characteristics. 10 Research during the past decade has demonstrated similar abnormal pain processing in FMS and related chronic pain syndromes. Patients with FMS have lowered mechanical and thermal pain thresholds, high pain ratings for noxious stimuli, and altered temporal summation of pain stimuli. 10-13 Physiological evidence of altered pain processing in FMS has been demonstrated by brain imaging 14-16 as well as CME available online at www.jama.com

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Significance of catechol-O-methyltransferase gene polymorphism in fibromyalgia syndrome

Cited by 234 publications

References 7 publications

Influence of catechol-O-methyltransferase (COMT) gene polymorphisms in pain sensibility of Brazilian fibromialgia patients

Influence of catechol-O-methyltransferase (COMT) gene polymorphisms in pain sensibility of Brazilian fibromialgia patients

Candidate gene studies of fibromyalgia: a systematic review and meta-analysis

Management of Fibromyalgia Syndrome

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