Significance of cell “observer” and protein source in nanobiosciences

Laurent, Sophie; Burtéa, Carmen; Thirifays, Coralie; Rezaee, Farhad; Mahmoudi, Morteza

doi:10.1016/j.jcis.2012.10.005

Cited by 73 publications

(70 citation statements)

References 34 publications

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“…It is well known that each patient has distinct plasma alterations that depend on the heterogeneity, period and severity of the disease, individual genetic variations and environmental factors. [20][21][22] Therefore, we hypothesized that alterations in plasma protein concentrations/structures are mediated by diseases, thus affecting the composition of the hard corona. Intriguingly, changes in the plasma protein composition due to disease alter the NP hard corona composition, which in turn gives the NPs an unpredictable biological identity (compared to the current in vitro data, which do not consider the plasma alteration effect) that determines their biological fate.…”

Section: Discussionmentioning

confidence: 99%

Personalized protein coronas: a “key” factor at the nanobiointerface

et al. 2014

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It is now well known that the primary interactions of biological entities (e.g., tissues and cells) with nanoparticles (NPs) are strongly influenced by the protein composition of the "corona" (i.e., the NP surface attached proteins). The composition of the corona strongly depends on the protein source (e.g., human plasma). Because the protein source determines the NP corona, it is reasonable to hypothesize that humans with specific disease(s) may have specific NP coronas. To test this hypothesis, we incubated two different hydrophobic/hydrophilic types of NPs ( polystyrene and silica) with plasma from human subjects with different diseases and medical conditions (e.g., breast cancer, diabetes, hypercholesterolemia, rheumatism, fauvism, smoking, hemodialysis, thalassemia, hemophilia A and B, pregnancy, common cold and hypofibrinogenemia). Our results demonstrate that the type of disease has a crucial role in the protein composition of the NP corona. Based on these results, we introduce the concept of the "personalized protein corona" (PPC) as a determinant factor in nano-biomedical science. This study will help researchers rationally design experiments based on the "personalized protein corona" for clinical and biological applications.

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Section: Discussionmentioning

confidence: 99%

Personalized protein coronas: a “key” factor at the nanobiointerface

et al. 2014

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“…Many proteins are associated with the protein corona for different patients, while some proteins only appear or disappear in the hard corona for particular diseases (Hajipour et al 2014). Therefore, it is important to take into account the personalization of protein corona in future studies for each individual patient with diverse heterogeneity, period and severity of the disease, individual genetic variations and environmental factors (Chrousos 2009;Hajipour et al 2014;Laurent et al 2013a). More specific and precise future investigations on protein coronas and their influence to biological responses from cell level to individual level are highly demanded.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Towards understanding of nanoparticle–protein corona

et al. 2015

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With the rapid developments of nanotechnology, chances of exposing nanoscale particles to humans (e.g., workers and consumers) also increase correspondingly, which raises serious concerns on their biosafety. Entrance of nanoparticles into diverse biological environment endows them with new and dynamic biological identities as the so-called nanoparticle-protein corona. Therefore, understanding the role of these nanoparticle-protein coronas and resulting biological responses is crucial, as it helps to clarify the biological mechanism and prevent the potential adverse effects of nanoparticles. In this review, we summarize the latest developments relating to the nanoparticle-protein interaction and corresponding biological responses, with an emphasis on the characterization methods, induced biological effects and possible molecular mechanisms. In addition, we overview both the challenges and opportunities (particularly in nanomedicine) raised by this entrance of nanoparticles into the living creatures, especially human beings, with some future perspectives based on our understanding.

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“…Full information on the protein corona compositions at the surface of various types of SPIONs is required to thoroughly understand their toxicities and biological fate. Although there are extensive reports on the biological responses (e.g., the cellular uptakes, the cellular signalling pathways, and the biodistribution) of SPIONs with various physicochemical properties22232425262728293031, there are few reports on the compositions of the protein corona at their surfaces32.…”

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Protein Corona Composition of Superparamagnetic Iron Oxide Nanoparticles with Various Physico-Chemical Properties and Coatings

Sakulkhu

Mahmoudi

Maurizi

et al. 2014

Sci Rep

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Because of their biocompatibility and unique magnetic properties, superparamagnetic iron oxide nanoparticles NPs (SPIONs) are recognized as some of the most prominent agents for theranostic applications. Thus, understanding the interaction of SPIONs with biological systems is important for their safe design and efficient applications. In this study, SPIONs were coated with 2 different polymers: polyvinyl alcohol polymer (PVA) and dextran. The obtained NPs with different surface charges (positive, neutral, and negative) were used as a model study of the effect of surface charges and surface polymer materials on protein adsorption using a magnetic separator. We found that the PVA-coated SPIONs with negative and neutral surface charge adsorbed more serum proteins than the dextran-coated SPIONs, which resulted in higher blood circulation time for PVA-coated NPs than the dextran-coated ones. Highly abundant proteins such as serum albumin, serotransferrin, prothrombin, alpha-fetoprotein, and kininogen-1 were commonly found on both PVA- and dextran-coated SPIONs. By increasing the ionic strength, soft- and hard-corona proteins were observed on 3 types of PVA-SPIONs. However, the tightly bound proteins were observed only on negatively charged PVA-coated SPIONs after the strong protein elution.

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Significance of cell “observer” and protein source in nanobiosciences

Cited by 73 publications

References 34 publications

Personalized protein coronas: a “key” factor at the nanobiointerface

Personalized protein coronas: a “key” factor at the nanobiointerface

Towards understanding of nanoparticle–protein corona

Protein Corona Composition of Superparamagnetic Iron Oxide Nanoparticles with Various Physico-Chemical Properties and Coatings

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