Significance of Detecting Circulating Hepatocellular Carcinoma Cells in Peripheral Blood of Hepatocellular Carcinoma Patients by Nested Reverse Transcription-polymerase Chain Reaction and its Clinical Value: A Retrospective Study

Liu, Yang; Wang, Yue-Ru; Wang, Long; Song, Ruimei; Zhou, Bo; Song, Zhijun

doi:10.1177/1660.18174

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…Most cases of HCC in whom AFPmRNA was detected were HBV seropositive (66.7%) compared to a previous study which indicated no association between AFPmRNA and hepatitis B infection (14). This is probably due to the high seroprevalence of HBV in Sudan.…”

Section: Discussionmentioning

confidence: 54%

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Predictive Biomarkers of Hepatocellular Carcinoma in Sudanese Patients with Liver Cirrhosis

2016

IJSR

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Background: Liver cirrhosis (LC) is a prevalent condition in Sudan and the main predisposing factor for hepatocellular carcinoma (HCC). This study assesses the effectiveness of the use of α-fetoprotein (AFP), ultrasound (US), AFPmRNA in addition liver enzymes as early predictors for hepatocellular carcinoma (HCC) in Sudanese patients with liver cirrhosis (LC). Methods: A Hundred patients with LC, 25 patients with confirmed HCC and 50 healthy controls were included in the study. Serum AFP, AFPmRNA and liver enzymes were determined annually in patients with liver cirrhosis in addition to abdominal U/S for 3 years. Serum AFP was determined using enzyme immunoassay (EIA) in patients and controls. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for detection of AFPmRNA. Results: During follow up hepatic nodules were identified in three liver cirrhotic patients (3%). AFP detection rate was higher (64%) at a low cut-off point (20ng/dl). the AFPmRNA had a very low sensitivity (24%) in HCC group. Only 3 patients developed HCC additionally to LC after follow up and all had positive AFPmRNA and AFP. Conclusions: In conclusion the combination of U/S, AFP and AFPmRNA would be the most effective tool for HCC screening in Sudan. However, AFPmRNA alone had the lowest detection rate and is therefore not recommended for screening.

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Section: Discussionmentioning

confidence: 54%

“…Nested RT-PCR was conducted and samples were analyzed by electrophoresis on a 15% polyacrylamide gel and stained with ethidium bromide for the specific bands. The design of external and inner pairs of primers were taken from previous research as follows (14).…”

Section: Methodsmentioning

confidence: 99%

Predictive Biomarkers of Hepatocellular Carcinoma in Sudanese Patients with Liver Cirrhosis

2016

IJSR

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“…Moreover, the content of AFP in serum has a significant correlation with the number of cancer cells and degree of differentiation of liver cancer. Serum AFP content is directly proportional to tumor diameter, and as its content increases, recurrence and metastasis are more likely to occur (32). Therefore, the content of AFP in serum has important value for the diagnosis and treatment of liver cancer and the prediction of recurrence.…”

Section: Figurementioning

confidence: 99%

A systematic review and meta-analysis on prognosis and survival of hepatocellular carcinoma with lung metastasis after hepatectomy

An¹,

Li²,

Mou³

et al. 2021

Ann Palliat Med

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Background:The most common metastatic site of hepatocellular carcinoma (HCC) is lung metastasis.When the patient has lung metastasis, the development of the disease will become very rapid. In severe cases, it will cause rapid death of the patient. Therefore, the treatment of HCC after lung metastasis has become a hot research topic. This study was designed to investigate the curative effect and prognostic factors of hepatectomy in patients with HCC with lung metastasis (LM) (HCC + LM patients) using meta-analysis.Methods: RevMan 5.3 software was used to screen literature based on randomized controlled studies on the curative effect and prognostic factors of hepatectomy for HCC + LM published between January 2000 to January 2020 in the PubMed, Web of Science, Spring, and Science Direct databases.Results: A total of 13 articles were included, comprising 754 patients in the H group and 1,201 patients in the NH group. Meta-analysis results showed that the maximum diameter of liver tumors in the H group

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“…Finally, circulating tumor cells (CTCs) recruited in peripheral blood were identified as a marker of hematogenous spread in various solid tumors [11]. So far, their predictive and prognostic value has been proven in breast [12], colorectal [13], lung [14], esophageal [15], liver [16], pancreatic [17], and prostate cancers [18]. Recently, the presence of positive CTCs was associated with deep myometrial invasion and lymph node positivity in endometrial cancer [19].…”

Section: Introductionmentioning

confidence: 99%

Circulating tumor cells as trigger to hematogenous spreads and potential biomarkers to predict the prognosis in ovarian cancer

et al. 2015

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Despite several improvements in the surgical field and in the systemic treatment, ovarian cancer (OC) is still characterized by high recurrence rates and consequently poor survival. In OC, there is still a great lack of knowledge with regard to cancer behavior and mechanisms of recurrence, progression, and drug resistance. The OC metastatization process mostly occurs via intracoelomatic spread. Recent evidences show that tumor cells generate a favorable microenvironment consisting in T regulatory cells, T infiltrating lymphocytes, and cytokines which are able to establish an "immuno-tolerance mileau" in which a tumor cell can become a resistant clone. When the disease responds to treatment, immunoediting processes and cancer progression have been stopped. A similar inhibition of the immunosuppressive microenvironment has been observed after optimal cytoreductive surgery as well. In this scenario, the early identification of circulating tumor cells could represent a precocious signal of loss of the immune balance that precedes cancer immunoediting and relapse. Supporting this hypothesis, circulating tumor cells have been demonstrated to be a prognostic factor in several solid tumors such as colorectal, pancreatic, gastric, breast, and genitourinary cancer. In OC, the role of circulating tumor cells is still to be defined. However, as opposed to healthy women, circulating tumor cells have been demonstrated in peripheral blood of OC patients, opening a new research field in OC diagnosis, treatment monitoring, and follow-up.

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Significance of Detecting Circulating Hepatocellular Carcinoma Cells in Peripheral Blood of Hepatocellular Carcinoma Patients by Nested Reverse Transcription-polymerase Chain Reaction and its Clinical Value: A Retrospective Study

Cited by 7 publications

References 15 publications

Predictive Biomarkers of Hepatocellular Carcinoma in Sudanese Patients with Liver Cirrhosis

Predictive Biomarkers of Hepatocellular Carcinoma in Sudanese Patients with Liver Cirrhosis

A systematic review and meta-analysis on prognosis and survival of hepatocellular carcinoma with lung metastasis after hepatectomy

Circulating tumor cells as trigger to hematogenous spreads and potential biomarkers to predict the prognosis in ovarian cancer

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