Abstract. The aim of the study was to specify by an impedimetric method the changes observed on the inner wall of central venous catheters for hemodialysis leading to the formation of a biological film. To evaluate these changes a patient-dialyzer model was built in which experimental parameters were kept closely similar to the clinical conditions of hemodialysis. The impedance spectra and SEM/EDS analysis of the biological layer deposited on the inner surface of the distal part of the catheter gave an insight into the structure of film formation and its chemical composition. Since an early detection of biofilm formation inside the distal part of the catheter is crucial for the safety of medical treatment and it usually prompts the implementation of antibiotic therapy. Developed impedimetric method can minimize the risk of infection and ensure the continuity of treatment.