Significance of genetic variations in developmental enamel defects of primary dentition in Polish children

Gerreth, Karolina; Zaorska, Katarzyna; Zabel, Maciej; Nowicki, Michał; Borysewicz−Lewicka, Maria

doi:10.1007/s00784-017-2115-1

Cited by 15 publications

(36 citation statements)

References 33 publications

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“…54 Interestingly, TUFT1 and TFIP11 can affect the formation of dental enamel. 55 Originally isolated as proteins that induce bone and cartilage formation, BMPs are considered to comprise almost a third of TGFβ. The BMP family is the largest within the TGFβ superfamily of growth factors, which also includes TGFβ, activin, inhibin, myostatin, and others.…”

Section: Discussionmentioning

confidence: 99%

A novel role in skeletal segment regeneration of extracellular vesicles released from periodontal-ligament stem cells

Diomede

D’Aurora

Gugliandolo

et al. 2018

IJN

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PurposeThe combination of oral derived stem cells and 3-D scaffolds is considered advantageous in bone repair. In particular, collagen membranes possess ideal biological properties and can support infiltration and proliferation of osteoblasts, promoting bone regeneration. Our study aimed to develop a new biocompatible osteogenic construct composed of a commercially available collagen membrane (Evolution [Evo]), human periodontal-ligament stem cells (hPDLSCs) enriched with extracellular vesicles (EVs), or polyethylenimine (PEI)-engineered EVs (PEI-EVs).MethodsOsteogenic ability and expression of osteogenic genes were evaluated in vitro in hPDLSCs cultured with or without Evo, with Evo and EVs, or PEI-EVs. In addition, the bone-regeneration capacity of Evo, Evo enriched with hPDLSCs, Evo enriched with hPDLSCs and EVs/PEI-EVs was investigated in rats subjected to calvarial defects.ResultsOur results showed that Evo enriched with EVs and PEI-EVs showed high biocompatibility and osteogenic properties in vitro and in vivo. In addition, quantitative reverse-transcription polymerase chain reaction demonstrated the upregulation of osteogenic genes, such as TGFB1, MMP8, TUFT1, TFIP11, BMP2, and BMP4, in the presence of PEI-EVs. Upregulation of BMP2/4 was confirmed for Evo enriched with PEI-EVs and hPDLSCs both in vitro by Western blot and in vivo by immunofluorescence.ConclusionOur results indicated that Evo enriched with hPDLSCs and PEI-EVs is able to promote a bone-regeneration process for the treatment of calvarium and ossification defects caused by accidental or surgery trauma. In particular, PEI-EVs had a significant role in activation of the osteogenic process.

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Section: Discussionmentioning

confidence: 99%

A novel role in skeletal segment regeneration of extracellular vesicles released from periodontal-ligament stem cells

Diomede

D’Aurora

Gugliandolo

et al. 2018

IJN

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“…Developmental defects of enamel were easily distinguished from white spot lesions on clinical grounds, based on the association of the carious lesion located on a tooth and areas of mature plaque [4].…”

Section: Clinical Examinationmentioning

confidence: 99%

“…Causative agents of localized defects include traumatic injuries, local infections, and irradiation. Defects with the generalized type of distribution may be caused by genetic disorders or by environmental intoxicants such as fluoride and dioxins as well as systemic disturbances, including perinatal and postnatal problems, malnutrition, infectious diseases, and a range of other medical conditions [1,[4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

A Study of Enamel Defects and Dental Caries of Permanent Dentition in School Children with Intellectual Disability

Gerreth

Opydo-Szymaczek

Borysewicz−Lewicka

2020

JCM

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Data concerning the prevalence of developmental enamel defects and their association with dental caries in individuals with intellectual disability are scarce. This paper aims to evaluate the prevalence and distribution of developmental enamel defects and dental caries in the permanent dentition of special-care school children from Poznan (Poland). Out of 1091 students attending all special-care schools in the city, the study covered 268 subjects with intellectual disability (mild, moderate, severe, and profound) with permanent dentition, aged 10–20. One calibrated dentist performed dental examinations. The Statistica Software v10 was used for statistical analysis, assuming the level of statistical significance p ≤ 0.05. Among the subjects of the study, 19.40% presented developmental enamel defects. The number of teeth with changes ranged from 1 to 28, with maxillary incisors most frequently affected. Students without developmental enamel defects had more teeth observed with active caries compared to those with such changes (10.92% vs. 7.82%, p < 0.01). The highest number of students with developmental defects of enamel was observed in the group of individuals with mild intellectual disabilities. The present study revealed that in special-care students from Poznan, enamel defects and dental caries were frequently observed. However, individuals with developmental enamel defects did not show higher dental caries indices.

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“…Shimizu et al had reported variations in those two genes associated with microhardness of the buccal surface at different SNPs (Shimizu et al, 2012 ). One possible explanation for these findings is that a SNP that is significantly associated with phenotypes is not necessarily the causative variant itself because it might be in a strong linkage disequilibrium with other true-positive SNPs that have not yet been identified in the gene region (Gerreth et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Instead, other SNPs in MMP20 and TUFT1 , including MMP20 rs1612069 and TUFT1 rs17640579, were associated with demineralization depth in our study. One possible explanation for these findings is that a SNP that is significantly associated with disease occurrence is not necessarily the causative variant because it might be linked to other true-positive SNPs that have not yet been identified in the gene that were passed down to the next generation (Gerreth et al, 2017 ). Environmental factors might also influence the effects of variations in these genes on caries susceptibility (Wang et al, 2012 ; Slade et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Variation in Enamel Formation Genes Influences Enamel Demineralization In Vitro in a Streptococcus mutans Biofilm Model

et al. 2017

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Genetic studies have shown that variations in enamel formation genes are associated with caries susceptibility. The aim of this study was to test in vitro whether variants in these genes are associated with dental enamel demineralization in a Streptococcus mutans biofilm model. DNA and enamel samples were obtained from 213 individuals. DNA was extracted from saliva, and 16 single nucleotide polymorphisms were analyzed. The physical and chemical properties of sound enamel samples and the mineral loss and the lesion depth of the demineralized enamel samples under cariogenic challenge were analyzed. Microhardness, enamel chemicals, mineral loss and demineralization depth were compared between different genotypes at each single nucleotide polymorphism. The GG genotype of TUFT1 (rs17640579) and the GT genotype of MMP20 (rs1612069) exhibited increased microhardness (p = 0.044 and 0.016, respectively). The GG genotype of AMBN (rs7694409) had a higher magnesium level, while the CT genotype of TFIP11 (rs2097470) had a lower magnesium level (p = 0.044 and 0.046, respectively). The GT genotype of MMP20 (rs1612069) had a higher calcium level (p = 0.034). The GG genotype of AMBN (rs13115627), the AG genotype of ENAM (rs12640848) and the AA genotype of MMP20 (rs2292730) had a lower phosphorus level (p = 0.012, 0.006, and 0.023, respectively). The GG genotype of AMBN (rs13115627) was also associated with a higher calcium-phosphorus ratio (p = 0.034). Individuals with the CC genotype of TFIP11 (rs134143) exhibited significantly more mineral loss (p = 0.011) and a deeper lesions (p = 0.042). Individuals with the TT genotype of TFIP11 (rs2097470) had more mineral loss (p = 0.018). Individuals with the GG genotype of TUFT1 (rs17640579) exhibited a shallower demineralization depth (p = 0.047). Individuals with the GT genotype of MMP20 (rs1612069) exhibited a shallower demineralization depth (p = 0.042). Individuals with the GG genotype of ENAM (rs12640848) exhibited less mineral loss (p = 0.01) and a shallower demineralization depth (p = 0.03). Genetic variations in TFIP11, TUFT1, MMP20, and ENAM influenced enamel demineralization in a Streptococcus mutans biofilm model.

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Significance of genetic variations in developmental enamel defects of primary dentition in Polish children

Cited by 15 publications

References 33 publications

A novel role in skeletal segment regeneration of extracellular vesicles released from periodontal-ligament stem cells

A novel role in skeletal segment regeneration of extracellular vesicles released from periodontal-ligament stem cells

A Study of Enamel Defects and Dental Caries of Permanent Dentition in School Children with Intellectual Disability

Variation in Enamel Formation Genes Influences Enamel Demineralization In Vitro in a Streptococcus mutans Biofilm Model

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