Significance of lymphocytic sister chromatid exchange frequencies in ovarian cancer patients

Dhar, Pawan K.; Devi, Sulochna; Rao, T. Ramesh; Kumārī, Ushā; Joseph, Anita; Kumar, Manoj; Nayak, Satheesha B.; Shreemati, Y.; Bhat, Smitha; Bhat, K. R.

doi:10.1016/0165-4608(95)00071-2

Cited by 14 publications

(10 citation statements)

References 10 publications

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“…Furthermore, a higher rate of cytogenetic damage in peripheral blood lymphocytes has been found in subjects affected by some kind of tumors and their relatives when compared with control groups. High baseline level of DNA damage may also point to an impaired DNA-repair ability among cancer patients, the probability that the neoplastic disease itself is associated with increased DNA damage or that these patients have a more fragile DNA than healthy individuals [36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Kopjar

Garaj‐Vrhovac

Milas

2001

Teratog Carcinog Mutagen

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The alkaline comet assay was employed to assess the pre-and post-treatment levels of in vivo DNA damage in peripheral blood leukocytes of cancer patients. During the study all patients were given antineoplastic drugs, mainly as polychemotherapy. To quantify the DNA damage, two different comet parameters were evaluated: the tail length and the tail moment. Our results indicate marked interindividual variations between baseline DNA damage in peripheral blood leukocytes recorded among cancer patients prior to the chemotherapy. After intravenous administration of various antineoplastic drugs, a significantly increased level of DNA damage in all cancer patients compared to their pre-treatment values was recorded The highest level of DNA damage was seen following administration of 5-fluorouracil, adriamycin, and cisplatin (FAP protocol). The results indicate that administration of antineoplastic drugs in standard protocols is accompanied by significant DNA damage in peripheral blood leukocytes. In order to diminish the potential risks of developing second neoplasms, a continuous biomonitoring of cancer patients after the ending of chemotherapy becomes important. Despite their limitations, present results confirm the usefulness of the alkaline comet assay as a sensitive biomarker of exposure that enables rapid and simple detection of primary DNA damage in peripheral blood leukocytes of cancer patients. Together with standard cytogenetic endpoints, the comet assay provides a powerful technique for the routine detection of critical DNA lesions produced after administration of antineoplastic drugs in the clinical settings. Teratogenesis Carcinog. Mutagen. 22:13-30, 2002.

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Section: Discussionmentioning

confidence: 99%

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Kopjar

Garaj‐Vrhovac

Milas

2001

Teratog Carcinog Mutagen

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“…This observation is consistent with current knowledge that H pylori infection induces cell hyperproliferation, inflammation, and genomic instability [53] . The frequency of SCE is increased in patients with carcinoma of cervix uteri, nasopharyngeal carcinoma, prostate carcinoma, ovarian carcinoma, acute leukemia, chronic lymphocytic leukemia and breast cancer [54][55][56][57][58][59] . Concerning gastric cancer, in one of the earliest studies SCE was increased to similar levels in patients with GC and those with CAG.…”

Section: Discussionmentioning

confidence: 99%

Alteration of sister chromatid exchange frequencies in gastric cancer and chronic atrophic gastritis patients with and without H pylori infection

Karaman

Binici²,

Kabalar³

et al. 2008

WJG

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AIM:To determine, by counting sister chromatid exchange (SCE) frequencies, whether genetic impairment and DNA damage have an effect on the pathogenesis of gastric cancer (GC). METHODS:Analysis of SCE is a cytogenetic technique used to show DNA damage as a result of an exchange of DNA fragments between sister chromatids. We analyzed SCE frequency in 24 patients with GC, 26 patients with chronic atrophic gastritis (CAG), and 15 normal controls. The presence of H pylori was confirmed by urease test, toluidine-blue stain and hematoxylin-eosin stain.RESULTS: SCE was significantly increased in H pylorinegative GC patients, and in H pylori -negative CAG patients compared with controls (7.41 ± 1.36 and 6.92 ± 1.20, respectively, vs 5.54 ± 0.8, P < 0.001). There was no difference in the SCE frequency between H pylorinegative GC patients and H pylori -negative CAG patients (P > 0.05). On other hand, the SCE frequencies in H pylori -positive GC patients were higher than those in H pylori -positive CAG patients (9.20 ± 0.94 vs 7.93 ± 0.81, P < 0.01). Furthermore, H pylori -positive GC patients had a higher SCE frequency than H pylorinegative GC patients (9.20 ± 0.94 vs 7.41 ± 1.36, P < 0.001). Similarly, a significant difference was detected between H pylori -positive CAG patients and H pylori -negative CAG patients (7.93 ± 0.81 vs 6.92 ± 1.20, P < 0.05). CONCLUSION:We suggest the increased SCE in patients reflects a genomic instability that may be operative in gastric carcinogenesis.

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“…1 B and C). In mammalian cells, the frequency of strand exchange correlates quite directly with genomic instability, cell death, and carcinogenic transformation and, in fact, is often used as a diagnostic and prognostic marker in cancer patients (10)(11)(12)(13). Thus, whereas the role of recombination is quite clear in reproductive cycles that tolerate variations among the quantity or genetic makeup of the progeny, when recombination occurs during asexual reproductive cycles the effects often appear to be detrimental.…”

Section: G Enetic Recombination Is Universal To All Organisms and It Ismentioning

confidence: 99%

Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination

Courcelle

Hanawalt

2001

Proc. Natl. Acad. Sci. U.S.A.

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Alternative reproductive cycles make use of different strategies to generate different reproductive products. In Escherichia coli, recA and several other rec genes are required for the generation of recombinant genomes during Hfr conjugation. During normal asexual reproduction, many of these same genes are needed to generate clonal products from UV-irradiated cells. However, unlike conjugation, this latter process also requires the function of the nucleotide excision repair genes. Following UV irradiation, the recovery of DNA replication requires uvrA and uvrC, as well as recA, recF, and recR. The rec genes appear to be required to protect and maintain replication forks that are arrested at DNA lesions, based on the extensive degradation of the nascent DNA that occurs in their absence. The products of the recJ and recQ genes process the blocked replication forks before the resumption of replication and may affect the fidelity of the recovery process. We discuss a model in which several rec gene products process replication forks arrested by DNA damage to facilitate the repair of the blocking DNA lesions by nucleotide excision repair, thereby allowing processive replication to resume with no need for strand exchanges or recombination. The poor survival of cellular populations that depend on recombinational pathways (compared with that in their excision repair proficient counterparts) suggests that at least some of the rec genes may be designed to function together with nucleotide excision repair in a common and predominant pathway by which cells faithfully recover replication and survive following UV-induced DNA damage.

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Significance of lymphocytic sister chromatid exchange frequencies in ovarian cancer patients

Cited by 14 publications

References 10 publications

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Assessment of chemotherapy‐induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

Alteration of sister chromatid exchange frequencies in gastric cancer and chronic atrophic gastritis patients with and without H pylori infection

Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination

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