Significance of microRNA 21 in gastric cancer

Sekar, Durairaj; Ravindran, Krishnan; Thirugnanasambantham, Krishnaraj; Baskaran, Rathinasamy; Ibrahim, Hairul-Islam Mohamed; Sekar, Punitha

doi:10.1016/j.clinre.2016.02.010

Cited by 63 publications

(38 citation statements)

References 46 publications

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“…MicroRNAs are involved in a series of tumorigenic processes, including cell proliferation, apoptosis, migration, and invasion. As reported in previous studies, many miRNAs are aberrantly expressed in GC tissues, serum, and cells, and these alterations are connected with the occurrence, progression, and prognosis of cancer . As post‐transcriptional regulators, miRNAs affect the expression of a large number of targeted mRNAs that encode proteins.…”

Section: Discussionmentioning

confidence: 76%

“…in previous studies, many miRNAs are aberrantly expressed in GC tissues, serum, and cells, and these alterations are connected with the occurrence, progression, and prognosis of cancer. 21,22 As posttranscriptional regulators, miRNAs affect the expression of a large number of targeted mRNAs that encode proteins. Thus, it is essential to identify the pathway connecting miRNAs with their target genes and regulated proteins.…”

Section: In Vivo Role Of Mir-155 and Tgfbr2 In Gastric Cancermentioning

confidence: 99%

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MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

Zhang

Sun

et al. 2018

Cancer Science

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Gastric cancer (GC) is one of the most common malignancies worldwide and has high morbidity and mortality rates. It is essential to elucidate the molecular events of GC proliferation and invasion, which will provide new therapeutic targets for GC. The inactivation of transforming growth factor‐β receptor 2 (TGFβR2) correlates with cancer cell growth and metastasis, but the mechanisms underlying the downregulation of TGFβR2 expression remain unknown. MicroRNAs (miRNAs) act as post‐transcriptional regulators and play a key role in the development of cancers. Bioinformatics analysis and luciferase reporter assays have shown that miR‐155 directly binds to the 3′‐UTR of TGFβR2 mRNA. In this study, we found that the TGFβR2 protein levels, but not mRNA levels, were downregulated in GC tissues, and the levels of miR‐155 were significantly increased in GC tissues. We deduced that miR‐155 was inversely correlated with TGFβR2 in GC cells. In vitro studies showed that overexpression of miR‐155 in SGC7901 inhibited the expression of TGFβR2 and then promoted GC cell proliferation and migration, whereas miR‐155 inhibitor showed opposite effects. In addition, the tumor‐suppressing function of TGFβR2 was verified by using siRNA and TGFβR2 overexpressing plasmids. The results showed that miR‐155 promotes cell growth and migration by negatively regulating TGFβR2. Thus, miR‐155‐regulated TGFβR2 as a potential therapeutic target in GC.

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Section: Discussionmentioning

confidence: 76%

Section: In Vivo Role Of Mir-155 and Tgfbr2 In Gastric Cancermentioning

confidence: 99%

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

Zhang

Sun

et al. 2018

Cancer Science

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“…miR-21 has been frequently reported to be aberrantly overexpressed in diverse tumors, including glioblastoma, breast cancer and malignant cholangiocytes (22,23). Recently, miR-21-5p was also reported to be overexpressed in gastric cancer (24). Previous studies demonstrated that miR-21-5p plays a tumor-promoting role in many types of cancer.…”

Section: Discussionmentioning

confidence: 99%

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

Liu

Fei

et al. 2018

Oncol Rep

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Pyruvate dehydrogenase A1 (PDHA1) is a component of the pyruvate dehydrogenase enzyme complex, which links glycolysis and the tricarboxylic acid cycle, and is important for cancer metabolism shift. PDHA1 downregulation has been revealed in several types of cancer to enhance glycolysis. However, the role of PDHA1 in gastric cancer remains largely unknown. In the present study, we found that PDHA1 was significantly downregulated in gastric cancer, and associated with poor prognosis. PDHA1 downregulation promoted gastric cancer glycolysis and cancer progression. miR‑21‑5p directly targeted PDHA1 to suppress PDHA1 expression, and promote glycolysis as well as cell proliferation in gastric cancer. Moreover, miR‑21‑5p was significantly upregulated in gastric cancer and negatively associated with PDHA1 expression in gastric cancer samples. Our results indicated that miR‑21‑5p targeted PDHA1 to regulate a metabolic switch and cancer progression in gastric cancer, and reveal the potential role of the miR‑21‑5p/PDHA1 axis in gastric cancer treatment.

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“…This was likely related to the oncogenic activity of miR-21 that can stimulate proliferation, migration, and invasion of cancer cells, including gastric cancer (Sekar et al , 2016). In the latter case, the molecule has been demonstrated to modulate the expression of multiple genes, such as epidermal growth factor receptor (EGFR), phosphatase and tensin homologue (PTEN), programmed cell death 4 (PDCD4), Fas ligand (FASLG), and reversion-inducing cysteine-rich protein with kazal motifs (RECK).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: the importance of miR-21 and miR-331

et al. 2017

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Background:High stability and disease-specific disarrangements suggest that microRNA molecules (miRNAs) present in body fluids are ideally suited for diagnostic applications, including gastric cancer (GC). However, the actual source of circulating miRNA biomarkers in GC has not been adequately evaluated, particularly in the Western populations that have some distinct characteristics compared with Asian patients.Methods:Twenty treatment-naive patients with GC along with 20 cancer-free controls were recruited. miRCURY LNA miRNA microarrays were used for miRNA expression profiling in primary tumours and adjacent healthy mucosa. Differentially expressed serum miRNAs were identified with a high throughput TaqMan OpenArray technology in tumour-draining veins of the portal system, as well as peripheral blood of the patients and controls.Results:Tissue profiling identified 108 sequences differentially expressed between primary tumours and adjacent mucosa (87 upregulated and 21 downregulated). Twenty miRNAs found in serum of GC patients showed expression levels higher than in controls. However, only seven of these molecules were overexpressed in primary tumours (miR-130a, miR-331, miR-19a, miR-223, miR-106a, miR-21, and miR-374). Moreover, expression of miR-331 and miR-21 was significantly higher in the peripheral circulation compared to tumour-draining veins of the portal system.Conclusions:The results indicate that the majority of potential serum miRNA biomarkers may originate from tissues other than the primary tumour.

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Significance of microRNA 21 in gastric cancer

Cited by 63 publications

References 46 publications

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

MicroRNA‐155 promotes gastric cancer growth and invasion by negatively regulating transforming growth factor‐β receptor 2

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: the importance of miR-21 and miR-331

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