Nitrogen content was estimated in rhizosphere of legume Halimodendron halodendron (Pall.) Voss along with Berberis integerrima Bunge as control in sandy loam soils of arid and semi-arid areas in Middle East every 2 years (2013-2019). Lepidium sativum seed was also cultivated in soil of shrubs rhizosphere for monitoring its performance and N content. Maximum rates of 1.05% year −1 and 1.54% year −1 for soil organic nitrogen (ON) and 0.50% year −1 and 0.66% year −1 for mineral nitrogen (MN) were observed in H. halodendron rhizospheres in arid and semi-arid areas, respectively. Moreover, the highest L. sativum performance occurred in H. halodendron rhizosphere. Significant relation also was observed between N content of L. sativum and N content of shrubs rhizosphere (p < 0.02; R 2 = 0.606 and 0.585 between SON and MN of soil, respectively, and L. sativum N content).