Significance of pre-transplant anti-HLA antibodies detected on an ELISA mixed antigen tray platform

Abstract

This study aims at examining the clinical impact, of antibodies detected on an ELISA mixed antigen tray format (LATM, One Lambda) in the absence of complement dependent cytotoxicity (CDC) positivity. All patients who underwent renal transplantation in 2007 and 2008 had their final pre-transplant sera retrospectively analyzed by the LATM assay. These patients were then followed-up with clinical, biochemical, and histopathological end points defined by elevation of serum creatinine and/or histopathological criteria. Among 164 patients who were studied, 149 received grafts from live related donors and 15, from deceased donors. 31 (19%) of the transplanted patients demonstrated pre-transplant anti-HLA IgG antibodies on the assay. Totally, 15 were positive for class I antibodies, 4 for class II antibodies, and 12 for both class I and class II antibodies. 44 patients (36%) experienced rejection. 8 out of 31 (26%) ELISA positive patients and 36 out of 133 (27%) ELISA negative patients experienced rejection. Among 15 patients who received deceased donor transplants, 4 were positive for ELISA, and 11 were negative. All 4 (100%) of the ELISA positive patients experienced rejection as compared to 3 out of 11 (27%) ELISA negative patients (P = 0.01). The ELISA LATM assay did not show any predictive value for rejection in our overall patient population; however, results in the specific setting of deceased donor transplants merit further exploration.

Fabricating a Low-cost and Low-immune Xenogeneic Aortic Valve Bio Prosthesis

“…Here the most signi cant challenge was endothelialization using host-speci c HLA type or immunologically matched cells. It is a critical problem for the patients to get an immunologically HLA-matched donor for organs like the heart and valves for transplant in emergencies [8]. The donated and HLAmatched transplanted aortic valves are not always safe, as this may already have many hidden glitches, including virological infections (HIV, HCV, HBV) [9].…”

Development of an experimental model of a decellularized kidney scaffold by perfusion mode and analyzing the three-dimensional extracellular matrix architecture by edge detection method

“…It is a great challenge for the clinicians and patients to get a matched (human leukocyte antigen [HLA]) donor for kidney transplant. [ 4 ] The donated and matched (HLA) transplanted kidneys have risk of virus infections transmission (HIV, HCV, and HBC). [ 5 ] In absence of proper registry, it is assumed that nearly 3 lakh renal patients need dialysis or renal transplant.…”