Significance of serum hepatitis C virus RNA levels in chronic hepatitis C

Lau, Johnson Y.N.; Davis, Gary L.; Kniffen, Jared C.; Qian, Keping; Urdea, M. S.; Chan, Cho-Yu; Neuwald, Paul; Wilber, Judith C.; Mizokami, Masashi

doi:10.1016/0140-6736(93)90635-t

Cited by 794 publications

(363 citation statements)

References 22 publications

Supporting

Mentioning

331

Contrasting

Unclassified

Order By: Relevance

“…In addition, our analysis shows that neither the activity of liver disease nor serum HCV RNA levels are related to HCV genotypes, whereas the activity of liver disease is related to serum HCV RNA levels. Our results support previous studies [9,43]. The issue of genotype pathogenicity remains to be demonstrated.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Pham

Martinot‐Peignoux

Mosnier

et al. 1995

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe pathogenic mechanisms that lead to chronic hepatitis C are unknown. As hepatitis C virus (HCV) has been shown to induce T cell response, we assessed whether a partictilar T lymphocyte subset could be preferentially detected in theliver of patients with chronic hepatitis C in relation to viraemia or HCV genotypes. The immunophenotypes of liver-derived lymphocytes were analysed in 26 patients by flow cytometry and immunohistochemistry. Viraemia was quantified by branched DNA assay. Using this assay, HCV RNA was not detectable in six patients. HCV RNA was detected in 20 patients, and titres ranged from 8 to 137 x 10''Eq/ml. Genotyping was performed using a line probe assay. Type la. lb. 2a, 3a and 4a were found to infect 2. 10. 2, 7 and 3 patients, respectively. The CD4 ^ /CD8 * ratio of liver-derived lymphocytes was significantly higher [P < 001) in patients with detectable viraemia than in patients without detectable viraemia. In contrast, neither the percentage of ^/i^ T lymphocytes nor that of CD2^CD57^ cells was different in the groups. When comparing the CD4^/CD8^ ratio, the percentage of-y/^ T lymphocytes or CD2^CD57' cells according to genotype, the differences were not significant. These results suggest that the CD4^/ CD8"^ratio of liver-derived lymphocytes is related to viraemia but not to HCV genotypes in patients with chronic hepatitis C. and that T lymphocytes may be involved in the pathogenesis of liver le.sions in chronic hepatitis C, Keywords hepatitis C virus chronic hepatitis C liver-derived lymphocytes lymphocyte subsets viraemia hepatitis C virus genotype

show abstract

Section: Discussionsupporting

confidence: 92%

“…Serum HCV RNA detection provides direct evidence for active viraemia. Viraemia quantification may be assessed by a new assay that uses a branched DNA (bDNA) signal amplification [9]. It has been suggested that quantitative study of HCV RNA may be useful since patients with low HCV viraemia are likely to respond to interferon therapy [10].…”

Section: Introductionmentioning

confidence: 99%

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Pham

Martinot‐Peignoux

Mosnier

et al. 1995

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…HCV genotypes were determined by means of polymerase chain reaction 18 methods and were expressed according to the nomenclature proposed by Simmonds et al 19 Serum HCV RNA was quantified using a branched DNA assay (Chiron HCV RNA, Chiron Corporation, Emeryville, CA). 20 The quantity of HCV RNA in the serum samples was expressed in million-genome equivalents (Meq) per milliliter. When the HCV RNA quantity was too low to be measured (F 0.5 mEq/mL) using branched DNA methods, the value was estimated as 0 (negative).…”

mentioning

confidence: 99%

Influence of HLA haplotypes on the clinical courses of individuals infected with hepatitis C virus

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The tendency of CH 50 to be depleted in normal serum was observed in those samples contained in fractions [23][24][25][26] (Fig. 5a).…”

Section: Cryoglobulin Analysismentioning

confidence: 99%

Hepatitis C virus (HCV)-induced IgG-IgM rheumatoid factor (RF) complex may be the main causal factor for cold-dependent activation of complement in patients with rheumatic disease

Wei

Yano

Kuroiwa

et al. 1997

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYA low serum complement level is commonly found in patients with rheumatic diseases. We evaluated 170 patients with such diseases to determine their serum levels of CH 50 , C3 and C4 protein. Persistent hypocomplementaemia was found in 19 of those patients, particularly in those with systemic lupus erythematosus (SLE). Cold-dependent activation of complement (CDAC) was demonstrated in nine of the 19 (47 . 4%), and six of the nine patients demonstrated infection with HCV (66 . 7%). The nine patients that exhibited CDAC had nearly normal haemolytic complement activity when the sera were separated either at 378C or in EDTA-treated plasma. Conversely, it markedly decreased, even to the point of being immeasurable, when the sera were separated at 4-218C. No significant deficiency in C3 and C4 protein levels was found in these patients. Clinical parameters other than levels of anti-HCV antibody, transaminase, and RF were not influenced by CDAC. In an attempt to isolate the causal factor for CDAC, we isolated IgG fractions from the CDAC patients by using a protein G column, in which case precipitates were collected from the eluates. The precipitates were mixed with normal serum and incubated at 4-218C for 18 h. A decrease in the level of CH 50 in normal serum was observed, which predominated (P < 0 . 001) when precipitates from HCV-infected patients were used. This indicated CDAC was possibly interrelated to the precipitates of such patients. This precipitate was proved to contain IgM besides IgG. It is therefore possible that an HCV-related IgG complex or an IgG-IgM RF complex may be formed at low temperature and be involved in activating the complement system in vitro.

show abstract

Significance of serum hepatitis C virus RNA levels in chronic hepatitis C

Cited by 794 publications

References 22 publications

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Influence of HLA haplotypes on the clinical courses of individuals infected with hepatitis C virus

Hepatitis C virus (HCV)-induced IgG-IgM rheumatoid factor (RF) complex may be the main causal factor for cold-dependent activation of complement in patients with rheumatic disease

Contact Info

Product

Resources

About