Significance of Species Sensitive Electrochemical Methods for the Analysis of Antineuractodermal Agent AE (Aloe-Emodin)

Abstract

Species sensitive Direct current (DCP) and differential pulse polarographic (DPP) methods have been developed and authenticated for the qualitative as well as quantitative analysis of antineuractodermal agent, Aloe-Emodin (AE) in natural origin, human blood sample and industrial samples. 10 mm AE produces a well defined polarographic reduction wave/peak in acetate buffer with Ε'Λ/Ερ = -1140 mv Vs SCE at pH 6.5 + 0.1, however, the EVil Ep value shifts to more electronegative value with increase in AE concentration due to change of the viscosity of the medium. The developed electrochemical methods have been standardized for the analysis of Aloe-Emodin (Antineuroactodermal agent) in pharmaceutical formulations. The electrochemical analysis has been supplemented by FTIR spectroscopic and HPLC methods. An isocratic high performance liquid chromatography (HPLC) method has also been developed and validated to determine AE in human blood plasma. The HPLC separation was carried out on symmetry shield RP18 a mobile phase of methanol -water-acetic acid (65:35:02) and UV detection of λβχ = 410 nm and Xem = 510 nm. The retention time of AE was 4.25 min.