Significance of superoxide dismutase (SOD) in human colorectal cancer tissue: Correlation with malignant intensity

Satomi, Akira; Murakami, Saburo; Hashimoto, Taiju; Ishida, Kiyoshi; Matsuki, Moriyuki; Sonoda, Masaru

doi:10.1007/bf02348662

Cited by 44 publications

(36 citation statements)

References 17 publications

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“…Ofner et al (1994) Furthermore. an increased SOD activity level of carcinomas was found to be associated with the malignant intensity of colorectal carcinomas (Satomi et al 1995). In our present study, however.…”

Section: Discussioncontrasting

confidence: 64%

Superoxide dismutases in relation to the overall survival of colorectal cancer patients

Janssen

Bosman²,

Sier³

et al. 1998

Br J Cancer

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Summary Reactive oxygen metabolites are implicated in the initiation and promotion of cancer. In addition, oxidant scavengers, such as manganese -(Mn-SOD) and copper/zinc -superoxide dismutase (Cu/Zn-SOD), are thought to contribute to colorectal cancer treatment response. In the present study, the prognostic significance of the Mn-and Cu/Zn-SOD antigen content of normal mucosa and carcinomas of 163 patients with colorectal cancer was evaluated in comparison with major clinicopathological parameters, with respect to the 5-year overall survival. The Mn-SOD content of carcinomas was found to be significantty higher than that of normal mucosa, whereas there was no difference in the Cu/Zn-SOD content between the normal mucosa and carcinomas. No association was demonstrable between the Mn-SOD and Cu/Zn-SOD content of the tissues and the assessed clinicopathological parameters (gender, age, localization, differentiation grade, diameter and Dukes' stage), with the exception of the Cu/Zn-SOD and the differentiation grade of the carcinomas. Univariate analysis showed that a high Mn-SOD content of carcinomas was associated with a poor 5-year overall survival of the patients with colorectal cancer. Multivariate analysis including all clinicopathological parameters revealed that this Mn-SOD parameter was prognostically independent. The Mn-and Cu/Zn-SOD content of normal mucosa and the Cu/Zn-SOD content of carcinomas were not associated with the overall survival of the patients. In conclusion, this study demonstrates that for patients with colorectal cancer the Mn-SOD content of colorectal carcinomas has a significant prognostic value that is independent from major clinicopathological parameters, including Dukes' stage.

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Section: Discussioncontrasting

confidence: 64%

Superoxide dismutases in relation to the overall survival of colorectal cancer patients

Janssen

Bosman²,

Sier³

et al. 1998

Br J Cancer

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“…It has been hypothesized that deficiencies in nutritional antioxidants may exacerbate damage associated with the increased production of H 2 O 2 resulting from the enhanced activity of Sod2. A variety of epidemiologic studies have also linked increases in Sod2 levels, independent of polymorphism, with significant increases in colon, gastric, lung, and breast cancers, as well as mesothelioma and glioblastoma (7,39,(47)(48)(49)(50)(51). Of these studies, the increases in Sod2 activity or mRNA from tumor to normal tissue vary.…”

Section: Discussionmentioning

confidence: 99%

Manganese Superoxide Dismutase Enhances the Invasive and Migratory Activity of Tumor Cells

et al. 2007

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Clinically significant elevations in the expression of manganese superoxide dismutase (Sod2) are associated with an increased frequency of tumor invasion and metastasis in certain cancers. The aim of this study was to examine whether increases in Sod2 activity modulate the migratory potential of tumor cells, contributing to their enhanced metastatic behavior. Overexpression of Sod2 in HT-1080 fibrosarcoma cells significantly enhanced their migration 2-fold in a wound healing assay and their invasive potential 3-fold in a transwell invasion assay. Severity of invasion was directly correlated to Sod2 expression levels and this invasive phenotype was similarly observed in 253J bladder tumor cells, in which Sod expression resulted in a 3-fold increase in invasion compared with controls. Further, migration and invasion of the Sod2-expressing cells was inhibited following overexpression of catalase, indicating that the promigratory/ invasive phenotype of Sod2-expressing cells is H 2 O 2 dependent. Sod2 overexpression was associated with a loss of vinculin-positive focal adhesions that were recovered in cells coexpressing catalase. Tail vein injections of Sod2-GFPexpressing HT-1080 cells in NCR nude mice led to the development of pulmonary metastatic nodules displaying high Sod2-GFP expression. Isolated tumors were shown to retain high Sod2 activity in culture and elevated levels of the matrix degrading protein matrix metalloproteinase-1, and a promigratory phenotype was observed in a population of cells growing out from the tumor nodule. These findings suggest that the association between increased Sod2 activity and poor prognosis in cancer can be attributed to alterations in their migratory and invasive capacity. [Cancer Res 2007; 67(21):10260-67]

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“…Both SOD and glutathione are antioxidants capable of scavenging free radicals or reactive oxidative species (ROS). Some reports have suggested that the accumulation of oxygen radicals in malignancies is actually accompanied by a reduction in SOD activity (50,51). In mouse myeloma cells, ˆ-3 fatty acids enhanced formation of superoxide anion, hydrogen peroxide and lipid peroxides but also caused reduced levels of antioxidant enzymes such as SOD, glutathione peroxidase (52).…”

Section: Discussionmentioning

confidence: 99%

The cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferation

Lee

Sit²,

Fan³

et al. 2010

Int J Oncol

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Abstract. Given the reported side effects associated with chemotherapy and surgical resection, dietary intervention with ˆ-3 polyunsaturated fatty acids (PUFAs) has been postulated to be an alterative way to prevent liver cancer progression and metastasis. We studied the effects of an ˆ-3 PUFA, docahexaenoic acid (DHA) on COX-2 expression and the cell cycle control machinery that co-ordinately regulate the HCC cells growth. Our data showed that DHA (0-200 μM) retarded proliferation of the human metastatic HCC cell line MHCC97L dose-dependently. In addition, inhibition of cyclin A/Cdk2 interfered with S-phase progression further in agreement with the result of bivariate flow cytometric analysis which indicated that DNA synthesis time (T s ) was significantly prolonged by DHA in MHCC97L. The N-myc oncogene, the heat shock proteins Hsp27 and glucose-related protein 78 (GRP78) as well as the antioxidant enzymes superoxide dismutase may play significant roles in the cell cycle control and reduced-proliferation of MHCC97L by DHA. Our data indicated that it is imperative to develop therapeutic strategy with ˆ-3 PUFA that simultaneously targets COX-2 and other cell cycle regulators in hepatocarcinogenesis. This study provides novel mechanistic insights into the modulation of DHA on human hepatocarcinoma.

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Significance of superoxide dismutase (SOD) in human colorectal cancer tissue: Correlation with malignant intensity

Cited by 44 publications

References 17 publications

Superoxide dismutases in relation to the overall survival of colorectal cancer patients

Superoxide dismutases in relation to the overall survival of colorectal cancer patients

Manganese Superoxide Dismutase Enhances the Invasive and Migratory Activity of Tumor Cells

The cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferation

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