Significance of the Concentration of Nonprotein-Bound Plasma Cortisol in Normal Subjects, Cushing's Syndrome, Pregnancy, and During Estrogen Therapy*†

Doe, Richard P.; Zinneman, Horace H.; Flink, Edmund B.; Ulstrom, Robert A.; Engst, Ruth; Lewis, Mary D.; Johnson, Janet

doi:10.1210/jcem-20-11-1484

Cited by 107 publications

(25 citation statements)

References 16 publications

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“…The lack of significant increase of plasma dialyzable cortisol after estrogen differs from other reports of an increase in nonprotein-bound corticosteroid in pregnancy or after estrogen treatment (22)(23)(24), but this may be due to the short period of treatment in this study.…”

Section: Discussioncontrasting

confidence: 99%

Parotid fluid cortisol and cortisone

Katz¹,

Shannon²

1969

J. Clin. Invest.

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A B S T R A C T Parotid fluid corticosteroids, substantially comprised of cortisol and cortisone, were previously demonstrated to rise to far greater levels 4 hr after administration of ACTH than they did in the third trimester of pregnancy, although the plasma total corticosteroid concentrations were similar in these two states. It was therefore suggested that only nonproteinbound corticosteroid gains access to parotid fluid. In the present study parotid fluid cortisol and cortisone and plasma dialyzable cortisol concentrations have been measured in normal men before and 2 hr after 40 U ACTH, and, in another group, before and after 10 days of diethystilbestrol (5 mg daily). Total plasma cortisol rose from a mean of 6.3 to 17.9 ,ug/100 ml after ACTH and from 14.6 to 39.4 mg/100 ml after the estrogen. However parotid fluid cortisol plus cortisone rose from 0.8 to 2.6 ,ug/100 ml after ACTH and to only 2.2 after estrogen. This rise resembled that of the plasma dialyzable cortisol (control 0.4, ACTH 1.8, estrogen 1.2 ug/ 100 ml) rather than the increase in total plasma cortisol which was over twice as high after estrogen as after ACTH. Thus parotid fluid corticosteroids seem to be a good measure of nonprotein-bound corticosteroid, the cortisol available to the cell. The total amount of cortisol plus cortisone excreted is approximately constant, independent of parotid fluid flow rate. Cortisone exceeds cortisol in parotid fluid in the basal state, but after ACTH the situation is reversed.

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Section: Discussioncontrasting

confidence: 99%

Parotid fluid cortisol and cortisone

Katz¹,

Shannon²

1969

J. Clin. Invest.

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“…They proposed that the protein should be known as transcortin and many authors continue to use this name. This identification of CBG as an alpha globulin was also confirmed by Daughaday (1958b) and by Doe et al (1960).…”

Section: Introductionsupporting

confidence: 67%

“…Early studies by Slaunwhite & Sandberg (1959a) and Doe et a/. (1960) showed that increased binding of cortisol to plasma proteins occurred in pregnancy, particularly in the last trimester.…”

Section: Pregnancymentioning

confidence: 99%

Human Corticosteroid Binding Globulin

Brien

1981

Clinical Endocrinology

181

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SUMMARY The literature on corticosteroid binding globulin (transcortin) in the human is reviewed under the following headings: physicochemical properties, biosynthesis, measurement, and physiological, pharmacological and pathological variations with particular emphasis of the effects of pregnancy and oral contraceptives. Finally, the physiological implications of corticosteroid binding globulin are discussed.

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“…For example, the non-equilibrium electrophoresis method used by Doe et al (1960) The period of oestrogen treatment used by Mills et al (1960) is too short to allow comparison. The dose of oestrogen used in the present work was sufficient to cause an increase in corticosteroid-binding globulin concentration, as judged by total cortisol levels.…”

Section: Discussionmentioning

confidence: 99%

Biologically Active Cortisol in Plasma of Oestrogen-treated and Normal Subjects

Cw¹

1969

BMJ

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Significance of the Concentration of Nonprotein-Bound Plasma Cortisol in Normal Subjects, Cushing's Syndrome, Pregnancy, and During Estrogen Therapy*†

Cited by 107 publications

References 16 publications

Parotid fluid cortisol and cortisone

Parotid fluid cortisol and cortisone

Human Corticosteroid Binding Globulin

Biologically Active Cortisol in Plasma of Oestrogen-treated and Normal Subjects

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