Significance of the Length of Carbon Nanotubes on the Bioelectrocatalytic Activity of Bilirubin Oxidase for Dioxygen Reduction

So, Keisei; Onizuka, Maki; Komukai, Takuji; Kitazumi, Yuki; Shirai, Osamu; Kano, Kenji

doi:10.1016/j.electacta.2016.01.117

Cited by 27 publications

(15 citation statements)

References 47 publications

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“…Recently, the effect of the length of MW-CNTs has also been evaluated 330 (Fig 23). This quite complex behavior was explained by considering determinant the ratio of hydrophobicity to density of carboxy groups.…”

Section: Cnt Properties Affecting Direct Electron Transfer To Mcosmentioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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Catalytic four-electron reduction of O to water is one of the most extensively studied electrochemical reactions due to O exceptional availability and high O/HO redox potential, which may in particular allow highly energetic reactions in fuel cells. To circumvent the use of expensive and inefficient Pt catalysts, multicopper oxidases (MCOs) have been envisioned because they provide efficient O reduction with almost no overpotential. MCOs have been used to elaborate enzymatic biofuel cells (EBFCs), a subclass of fuel cells in which enzymes replace the conventional catalysts. A glucose/O EBFC, with a glucose oxidizing anode and a O reducing MCO cathode, could become the in vivo source of electricity that would power sometimes in the future integrated medical devices. This review covers the challenges and advances in the electrochemistry of MCOs and their use in EBFCs with a particular emphasis on the last 6 years. First basic features of MCOs and EBFCs are presented. Clues provided by electrochemistry to understand these enzymes and how they behave once connected at electrodes are described. Progresses realized in the development of efficient biocathodes for O reduction relying both on direct and mediated electron transfer mechanism are then discussed. Some implementations in EBFCs are finally presented.

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Section: Cnt Properties Affecting Direct Electron Transfer To Mcosmentioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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“…Although some suitable redox mediators may assist electron transfer [34,35], several drawbacks arise [1-4, 10, 34, 35]: (a) the toxicity of the mediator, (b) leakage of the mediator, and (c) cell voltage loss to set up the driving force in the electron transfer between the enzyme and mediator. Therefore, multiple studies, such as creating novel electrode materials [36][37][38][39][40][41][42], functionalizing electrode surface [9,[43][44][45][46][47][48][49][50][51], and protein engineering [52][53][54], to improve DET-type bioelectrocatalysis have been reported.…”

Section: Introductionmentioning

confidence: 99%

Dual gas-diffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room temperature

Xia

Kitazumi

et al. 2016

Journal of Power Sources

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A membraneless direct electron transfer (DET)-type dihydrogen (H2)/air-breathing biofuel cell without any mediator was constructed wherein bilirubin oxidase from Myrothecium verrucaria (BOD) and membrane-bound [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F (MBH) were used as biocatalysts for the cathode and the anode, respectively, and Ketjen black-modified water proof carbon paper (KB/WPCC) was used as an electrode material. The KB/WPCC surface was modified with 2-aminobenzoic acid and p-phenylenediamine, respectively, to face the positively charged electron-accepting site of BOD and the negatively charged electron-donating site of MBH to the electrode surface. A gas-diffusion system was employed for the electrodes to realize high-speed substrate supply. As result, great improvement in the current density of O2 reduction with BOD and H2 reduction with MBH were realized at negatively and postively charged surfaces, repectively. Gas diffusion system also supressed the oxidative inactivation of MBH at high electrode potentials. Finally, based on the impoved bioanode and biocathode, a dual gas-diffusion membrane-and mediatorless H2/air-breathing biofuel cell was constructed. The maximum power density reached 6.1 mW cm −2 (at 0.72 V), and the open circuit voltage was 1.12 V using 1 atm of H2 gas as a fuel at room temperature and under passive and quiescent conditions.

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“…These excellent results are mostly attributed to the good dispersion of the CNT on the carbon fibers of CF, the suppressed deactivation of enzymes, and the suppressed leakage of enzymes from the CNT/TX/CFE into a solution. To prove this speculation, we evaluated the catalytic reaction rate constants (k cat ) of GOx on the CNT/CTAB and CNT/TX layer formed on Pt disc electrode by Koutecky‐Levich equation to be 1.33 s −1 and 3.47 s −1 , respectively (see test conditions and data in Figure S2). Although the former rate constant 1.33 s −1 is much lower than the rate constant 3.1 s −1 which is reported previously for other carbon‐fiber‐based GOx immobilized electrodes, the latter one 3.47 s −1 is higher than the reported result.…”

Section: Resultsmentioning

confidence: 99%

Multi‐Enzyme Immobilized Anodes Utilizing Maltose Fuel for Biofuel Cell Applications

et al. 2018

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Multi‐enzyme immobilized carbon‐felt electrodes are fabricated for application as a bioanode in biofuel cells to utilize both maltose and glucose as the fuel. The unique combination of three enzymes (maltase, mutarotase, and glucose oxidase) enables us to utilize maltose as the fuel for the bioanode. The new electrode based on carbon felt (CF) demonstrates a high oxidation current density of 6.5 mA cm−2 at 0.34 V vs. Ag/AgCl in a neutral phosphate buffer solution containing 0.025 mol dm−3 (≡M) maltose in a half‐cell configuration. Furthermore, we improve the bioanode performance by changing the surfactant from cetyltrimethylammonium bromide (CTAB) to Triton X‐100® (TX), which is used as a carbon nanotube (CNT) dispersant in the bioanode preparation process. A superior current density of 17 mA cm−2 at 0.34 V vs. Ag/AgCl is demonstrated with the multi‐enzyme bioanode by using TX as the CNT dispersant, owing to the good dispersion of CNTs attached to CF and the reduced deactivation and leakage of the enzymes. A maltose/O2 biofuel cell, composed of the multi‐enzyme immobilized bioanode and a biocathode based on bilirubin oxidase and mediator, delivers a maximum power density of 2.3 mW cm−2 and an open‐circuit voltage of 0.69 V in 0.2 M phosphate buffer solution containing 50 mM maltose.

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Significance of the Length of Carbon Nanotubes on the Bioelectrocatalytic Activity of Bilirubin Oxidase for Dioxygen Reduction

Cited by 27 publications

References 47 publications

O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells

Dual gas-diffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room temperature

Multi‐Enzyme Immobilized Anodes Utilizing Maltose Fuel for Biofuel Cell Applications

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Significance of the Length of Carbon Nanotubes on the Bioelectrocatalytic Activity of Bilirubin Oxidase for Dioxygen Reduction

Cited by 27 publications

References 47 publications

O2Reduction in Enzymatic Biofuel Cells

O2Reduction in Enzymatic Biofuel Cells

Dual gas-diffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room temperature

Multi‐Enzyme Immobilized Anodes Utilizing Maltose Fuel for Biofuel Cell Applications

Contact Info

Product

Resources

About

O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells