Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study

Abstract

Our results suggest the interest of the serum CTX-II monitoring for the OA progression and the relevance of the multiple time point analysis of this biomarker. Moreover, they address the question of the importance of correctly choosing the age of the animals used in the pre-clinical studies of OA.

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

“…These results were consistent with those of Dufield et al,15 showing that the levels of aggrecan neoepitopes ARGS and AGEG were elevated in the synovial fluid of rat knee after intraarticular (IA) injection of TNF-α. 31 Moreover, synovial fluid ARGS levels increased following surgically induced joint instability in the rat meniscal tear (MT) model.…”

“…20 In addition, in all the groups at 2 and 4 weeks after surgery, the Osteoarthritis Research Society International (OARSI) histological score was slightly but significantly correlated positively with the C2C/ CPII ratios. 15 In ACLT rats, serum CTX-II and COMP levels were increased and only CTX-II correlated with the modified Mankin score. 21 In ACLT and MMX rat model, low-intensity pulsed ultrasound (LIPUS) does not affect the degradation of type II collagen at 7 and 14 days after the operation.…”

“…In young animals, CTX-II levels increased rapidly and reached a maximum at week 2 and then, decreased over time until the week 20. 15 In experimental OA induced by anterior and posterior cruciate ligaments transection and medial meniscus excision, Zuo et al 16 investigated the evolution of serum bone morphogenetic protein-2 (BMP-2), CTX-II, and cartilage oligomeric protein (COMP). The BMP-2 levels were increased at various times after surgery, and significant differences were observed between the OA and the control groups.…”

Osteochondral Repair Using Porous Three-dimensional Nanocomposite Scaffolds in a Rabbit Model

“…Given the fact that the aim of the presented research was not to induce osteoarthritis but to study biomaterial implantation, the obtained constant CTX-II values in all study and control animals seem positive and evidence the absence of severe inflammation and osteoarthritis. This observation is consistent with results reported by other authors (29,53).…”

“…In the presented study, BAP, CTX-II, osteocalcin, and PINP concentrations were measured. The study of the respective biomarker levels relevant to bone turnover and reconstruction cartilage provided information on the advancement of changes affecting the skeletal system (29,30). The elevated concentrations of particular markers reflected an increased resorption within bone tissue and its intensive synthesis.…”