Significance of the V‐type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level

Dietz, Karl‐Josef; Tavakoli, Nastaran; Kluge, Christoph; Mimura, Tetsuro; Sharma, Sumita; Harris, Gary C.; Chardonnens, Agnes N.; Golldack, Dortje

doi:10.1093/jexbot/52.363.1969

Cited by 303 publications

(187 citation statements)

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“…Similar nonstoichiometric changes in subunit abundance have been reported in yeast and in mammalian renal tissue, where it is thought that changes in specific subunits of the protein complex can regulate assembly and/or coupling efficiency (Valles et al, 2005;Cipriano et al, 2008), without changing the final subunit stoichiometry. In plants, noncoordinated regulation of VHA subunits in tonoplast fractions has been suggested to be important for stress tolerance (Dietz et al, 2001), although how this is achieved is not addressed. An increase in VHA-B and not VHA-A could also indicate a previously unknown, V-ATPaseindependent function for a particular VHA-B isoform, of which there are three expressed in plants (Sze et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…VHA subunits A, B, C, D, E, F, G, and H make up the V 1 sector, and subunits a, b, c, d, and e compose the V o sector (Cipriano et al, 2008). In both salt-tolerant halophytes and salt-sensitive glycophytes, sodium regulates the expression at the transcript and protein levels for the tonoplast NHXs (Shi and Zhu, 2002;Yokoi et al, 2002) and different subunits of the V-ATPase Tsiantis et al, 1996;Dietz et al, 2001;Vera-Estrella et al, 2005). In addition, their transport activity has been shown to increase under salt stress (Reuveni et al, 1990;Barkla et al, 1995;Qiu et al, 2004;Vera-Estrella et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

et al. 2009

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To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na + sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H + -ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H + -pump activity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

et al. 2009

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“…40 The ability to respond to salinity stress with changes in the gene expression of the vacuolar ATPase might be a prerequisite and a characteristic of salt tolerance in plants. 60,61 It has been shown that the transcript levels of some subunits are upregulated in response to salt stress. In fully expanded leaves of M. crystallinum, 8 h after salt treatment, there was an increase in the transcript levels of subunit c mRNA but not of subunit A or B, 62 which correlates well with the observed increase in activity of the V-H + -ATPase in vesicles from leaf mesophyll tissue from plants treated with salt, 51 whereas in roots and young leaves, mRNA levels for all the three subunits increased about 2-fold compared to control plants.…”

Section: Regulation Of V-h + -Ppase and V-h + -Atpase Activity By Saltmentioning

confidence: 99%

Regulation by salt of vacuolar H⁺-ATPase and H⁺-pyrophosphatase activities and Na⁺/H⁺exchange

Silva

Gerós

2009

Plant Signaling & Behavior

165

102

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Over the last decades several efforts have been carried out to determine the mechanisms of salt homeostasis in plants and, more recently, to identify genes implicated in salt tolerance, with some plants being successfully genetically engineered to improve resistance to salt. It is well established that the efficient exclusion of Na + excess from the cytoplasm and vacuolar Na + accumulation are the most important steps towards the maintenance of ion homeostasis inside the cell. Therefore, the vacuole of plant cells plays a pivotal role in the storage of salt. After the identification of the vacuolar Na + /H + antiporter Nhx1 in Saccharomyces cerevisiae, the first plant Na + /H + antiporter, AtNHX1, was isolated from Arabidopsis and its overexpression resulted in plants exhibiting increased salt tolerance. Also, the identification of the plasma membrane Na + /H + exchanger SOS1 and how it is regulated by a protein kinase SOS2 and a calcium binding protein SOS3 were great achievements in the understanding of plant salt resistance.

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“…Furthermore, Cr promotes reduction of leaf area TOLERANCE AND PROSPECTION OF PHYTOREMEDIATOR WOODY SPECIES and biochemical changes responsible for the inhibition of chlorophyll synthesis (Vajpayee et al, 1999) and disorganization of the chloroplast ultrastructure (Panda and Choudhury, 2005). Chromium stress also causes leaf chlorosis and necrosis (Barbosa et al, 2007), oxidative damages in biomolecules such as lipids and proteins (Vajpayee et al, 2002), disturbances in mineral nutrition (Barbosa et al, 2007), increase in glutathione and ascorbic acid production (Shanker, 2003), alterations in the metabolic pool that intermediates the production of phytochelatins and histidine, interference in the activity of nitrate reductase (Panda and Patra, 2000), root Fe 3+ reductase (Shanker et al, 2004), plasma membrane H + -ATPase (Dietz et al, 2001), Na 2+ /K + -dependent ATPase (Pauls et al, 1980), Ca 2+ -dependent ATPase (Serpersu et al, 1982), alkaline phosphatases (Viola et al, 1980), superoxide dismutase, catalase and peroxidase (Samantaray et al, 2001) and, eventually, plant growth reduction, hindering its development and, finally, being able to cause its death ( Barbosa et al, 2007).…”

Section: Coppermentioning

confidence: 99%

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

Almeida

Valle

Mielke

et al. 2007

Braz. J. Plant Physiol.

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High concentrations of Cd, Pb, Cu and Cr can cause harmful effects to the environment. These highly toxic pollutants constitute a risk for aquatic and terrestrial life. They are associated with diverse bioavailable geochemical fractions, like the water-soluble fraction and the exchangeable fraction, and non-available fractions like those associated with the crystalline net of clays and silica minerals. Depending upon their chemical and physical properties we can distinguish different mechanisms of metal toxicity in plants, such as production of reactive oxygen species from auto-oxidation, blocking and/or displacement of essential functional groups or metallic ions of biomolecules, changes in the permeability of cellular membranes, reactions of sulphydryl groups with cations, affinity for reactions with phosphate groups and active groups of ADP or ATP, substitution of essential ions, induction of chromosomal anomalies and decrease of the cellular division rate. However, some plant species have developed tolerance or resistance to these metals naturally. Such evolution of ecotypes is a classic example of local adaptation and microevolution, restricted to species with appropriate genetic variability. Phytoremediator woody species, with (i) high biomass production, (ii) a deep root system, (iii) high growth rate, (iv) high capacity to grow in impoverished soils, and (v) high capacity to allocate metals in the trunk, can be an alternative for the recovery of degraded soils due to excess of metallic elements. Phytoremediation using woody species presents advantageous characteristics as an economic and ecologically viable system, making it an appropriate, practical and successful technology. Key-words: decontamination, heavy metals, hyperaccumulation, phytodegradation, phytostabilization, phytotoxicity Tolerância e prospecção de espécies lenhosas fitorremediadoras de Cd, Pb, Cu e Cr: Altas concentrações de Cd, Pb, Cu e Cr podem provocar efeitos danosos ao ambiente. Esses poluentes altamente tóxicos constituem um risco para a vida aquática e terrestre. Encontram-se associados às diversas frações geoquímicas biodisponíveis, a exemplo da fração solúvel em água e da fração trocável, e as não disponíveis como a fração associada à rede cristalina de argilas e de minerais silicatados. Conforme as suas propriedades químicas e físicas podem-se distinguir mecanismos diferentes de toxicidade de metais em plantas, tais como produção de espécies reativas de oxigênio pela auto-oxidação, bloqueio e, ou, deslocamento de grupos funcionais ou de íons metálicos essenciais de biomoléculas, mudanças na permeabilidade de membranas celulares, reações de grupos sulfidrílicos com cátions, afinidade para reações com grupos fosfatos e grupos ativos de ADP ou ATP, substituição de íons essenciais, indução de anomalias cromossomais e decréscimo da taxa de divisão celular. Várias espécies vegetais desenvolveram, naturalmente, tolerância ou resistência a esses metais. Essa evolução de ecótipos é exemplo clássico de adaptação local e de microevolução,...

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Significance of the V‐type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level

Cited by 303 publications

References 53 publications

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

Regulation by salt of vacuolar H⁺-ATPase and H⁺-pyrophosphatase activities and Na⁺/H⁺exchange

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

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Significance of the V‐type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level

Cited by 303 publications

References 53 publications

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

Regulation by salt of vacuolar H+-ATPase and H+-pyrophosphatase activities and Na+/H+exchange

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

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Regulation by salt of vacuolar H⁺-ATPase and H⁺-pyrophosphatase activities and Na⁺/H⁺exchange