Significant age‐ and gender‐related variability of main lymphocyte subsets in paediatric patients: Latvian data

Nikulshin, Sergey; Kundzina, Linda; Tolstikova, Iveta; Grāvele, Dagne; Prokofjeva, Tatjana; Gardovska, Dace

doi:10.1111/sji.12696

Cited by 2 publications

(1 citation statement)

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“…Previous reports have suggested that the single platform test using TruCount tubes reduces the difference between inter- and intra-laboratory [ 11 ]. However, most of the current researches proposed so far have established the reference ranges by dual-platform method [ 5 , 12 , 13 ]. Variations in the number and percentage of B cell subsets have been regarded to be influenced by ethnicity, gender, age and environmental factors [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the deep immune profiling of B cell subsets between healthy adults and Sjögren’s syndrome

et al. 2022

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Objectives Detailed analysis targeting B cell subgroups was considered crucial in monitoring autoimmune diseases and treatment responses. Thus, precisely describing the phenotypes of B cell differentiation and their variation in primary Sjögren’s syndrome (pSS) is particularly needed. Methods To characterize the proportions and absolute counts of B cell subsets, peripheral blood from 114 healthy adults of China (age range: 19–73 years) and 55 patients with pSS were performed by flow cytometry and CD19, CD20, CD24, CD27, CD38 and IgD were used as surface markers to identify B cell mature process. Age- and gender-stratified analyses were then carried out to improve the interpretation of B cell subsets. Results The assessments from healthy adults showed that the proportion of naive B cells presented a significant increase with age. A reversal trend was noted that the percentage of B10 decreased markedly with age. In addition, analysis based on gender showed that the relative percentage and number of naive B cells were higher in females than in males whereas the proportions of switched memory B cells and B10 cells were decreased in female. Patients with pSS exhibited a significant expansion in naïve B cells and unswitched memory B cells, accompanied with decreased switched memory B cells and B10 cells, which were identified to be associated with autoantibody production. Conclusions Our study presented a reliable analysis by flow cytometry to cover the principal B cell subtypes. These different stages of B lymphocytes may have implications for evaluating the activation of pSS and other autoimmune diseases and treatment efficacy. KEY MESSAGES B cell subsets play a pivotal role in the pathogenesis of primary Sjögren’s syndrome (pSS) and other autoimmune diseases. A practical and accurate flow cytometry method to profile B cell phenotypes in peripheral blood of healthy adults is especially essential. Additionally, we presented reliable reference ranges for B cell subsets in regards to the local population. Age- and gender-related analyses are available to better understand their influence in immune status and treatment outcome. The distribution of B-cell subsets is found substantially altered in patients with pSS, bringing novel avenues for pSS research in the future.

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