Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Redwan, Elrashdy M.; El-Baky, Nawal Abd; Al-Hejin, Ahmed M.; Baeshen, Mohammed N.; Almehdar, Hussein A.; Elsaway, Abdulrahman; Gomaa, A. M.; Almasaudi, Saad B.; Al-Fassi, F. A.; AbuZeid, Isam Eldin; Uversky, Vladimir N.

doi:10.1016/j.resmic.2016.04.006

Cited by 42 publications

(53 citation statements)

References 74 publications

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“…28 Lactoferrin has potential as a therapeutic agent against methicillin-resistant Staphylococcus aureus (MRSA) either alone or combination with other antimicrobial drug. 29 Similar with lactoferrin, the molecular weight of lactoperoxidase is also varies according to species. Previous study reported the molecular weights of lactoperoxidase in camel and bovine milk are 78 kDa and 72.5 kDa, respectively.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Lactoferrin and Lactoperoxidase from the Colostrum of Indonesian Ettawa Crossbred Goat

et al. 2017

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Background: Lactoferrin is a glycoprotein of the transferrin family which has multifunctional properties and useful for clinical and commercial application. It has some advantages as an immune system modulation, antibacterial activity and antioxidant in infant and adult as well as in animal. Lactoperoxidase is one of the most prominent enzymes in milk and catalyzes the inactivation of a wide range of microorganisms. Both lactoferrin and lactoperoxidase are expressed in most biological fluids, including colostrum. Chemical components of colostrum may vary along the days after parturition. The study of lactoferrin and lactoperoxidase from dairy goat is very limited. This study aimed to isolate and identify characteristics (the molecular weight and concentrations) of lactoferrin and lactoperoxidase from Indonesian native Ettawa crossbred goat colostrum. Method: The isolation and purification of lactoferrin and lactoperoxidase was conducted by an ion-exchange chromatography (SP-sepharose). Other compositions of colostrum, protein, fat and lactose concentration, were also determined. Results: The result showed that lactoferrin and lactoperoxidase concentrations in goat colostrum on the first day (317.3 mg/L and 204 mg/L) were higher than colostrum on the second day (190,5 mg/L and 61.9 mg/L) postpartum. The molecular weight of lactoferrin and lactoperoxidase were 75 and 72 kDa. Protein, fat and lactose concentrations of colostrum on the first day postpartum (6.5%, 5.7% and 4.9%) were higher than those on the second day (4.9%, 4.4% and 4.1%). Conclusion: A high amount of lactoferrin and lactoperoxidase can be isolated from the goat colostrum on its first day of postpartum.

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Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Lactoferrin and Lactoperoxidase from the Colostrum of Indonesian Ettawa Crossbred Goat

et al. 2017

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“…We have not examined the strain of S. aureus used in this study for its susceptibility to either lactoferrin or lactoferricin in solution. However, the MIC level of lactoferricin for other ocular isolates of S. aureus was high at between ≥1.28 mg/mL, and for human lactoferrin growth of methicillin‐resistant S. aureus occurred at concentrations between 1.5 and 3 mg/mL . Again, these results suggest a more potent activity of the surface‐bound proteins against S. aureus .…”

Section: Discussionmentioning

confidence: 94%

Antimicrobial activity of immobilized lactoferrin and lactoferricin

et al. 2016

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Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0.05) in nitrogen on the glass surface. The immobilization of both proteins slightly increased the overall hydrophobicity of the glass. Both lactoferrin and lactoferricin immobilized on glass significantly (p < 0.05) reduced the numbers of viable bacterial cells adherent to the glass. For P. aeruginosa, the immobilized proteins consistently increased the percentage of dead cells compared to the total cells adherent to the glass surfaces (p < 0.03). Lactoferrin and lactoferricin were successfully immobilized on glass surfaces and showed promising antimicrobial activity against pathogenic bacteria. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2612-2617, 2017.

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“…In vitro studies have delivered promising results supporting the use of camel milk against HCV infection (22,26,27,32–42), prompting the present in vivo investigation. The present study aimed to determine whether or not camel milk has therapeutic effects on HCV in infected Egyptian patients who did not take any medication in the 6 months prior to participation in the current study.…”

Section: Introductionmentioning

confidence: 94%

Influence of camel milk on the hepatitis C virus burden of infected patients

El‐Fakharany

El-Baky

Linjawi

et al. 2017

Experimental and Therapeutic Medicine

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Hepatitis C virus (HCV) infection represents a world health problem and no protective vaccine or effective drug currently exists. For economic reasons, many patients use traditional medicines to control the infection. In Egypt, camel milk is one of the traditional medicines widely consumed by patients infected with HCV. The present study aimed to evaluate the efficacy of camel milk in the treatment of patients infected with HCV. Whole camel milk from a local farm was administered to patients for 4 months (250 ml/day/patient). Patient sera were collected prior to and following camel milk drinking, and three markers were set-up for sera-evaluation. The three markers indicating the effect of camel milk on HCV infection were: Liver function assays [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)]; a viral load assay; and anti-HCV antibodies profile and isotyping against synthetic HCV epitopes. Camel milk demonstrated the ability to improve general fatigue, health and liver function (ALT and AST levels); ALT was reduced in ~88% of patients and AST was reduced in all patients subsequent to drinking camel milk for four months. The majority of patients responded positively to camel milk treatment; RNA viral load decreased in 13 out of the 17 patients (76.47%) and one patient exhibited undetected viremia following camel milk treatment. The anti-HCV antibodies profile and isotyping were significantly decreased (P<0.05) in immunoglobulin (Ig)G1 following treatment in 70–76% of patients. However, the treatment was ineffective in 23.53% of patients who experienced no reduction in RNA viral load following treatment with camel milk. In conclusion, whole camel milk treatment demonstrated efficacy in vivo; the viral load in the majority of patient sera was reduced and the IgG isotype profile was converted to Th1 immunity.

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Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Cited by 42 publications

References 74 publications

Isolation and Identification of Lactoferrin and Lactoperoxidase from the Colostrum of Indonesian Ettawa Crossbred Goat

Isolation and Identification of Lactoferrin and Lactoperoxidase from the Colostrum of Indonesian Ettawa Crossbred Goat

Antimicrobial activity of immobilized lactoferrin and lactoferricin

Influence of camel milk on the hepatitis C virus burden of infected patients

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