Significant Association of Chitinase 3-like 1 Genotypes to Asthma Risk in Taiwan

Cancer Genomics Proteomics

Shih

et al. 2021

Self Cite

Background/Aim: Xeroderma pigmentosum complementation group C (XPC) is reported to play important roles in DNA integrity and genomic instability, however, the contribution of XPC to oral carcinogenesis is largely uncertain. Therefore, we aimed at examining the contribution of XPC genotypes to oral cancer. Materials and Methods: The genotypes of XPC rs2228001 and rs2228000 were examined among 958 oral cancer patients and 958 control subjects by polymerase chain reaction-restriction fragment length polymorphism methodology and corresponding DNA repair capacity was checked. Results: First, the percentages of XPC rs2228001 AC and CC were higher among oral cancer patients than controls. Second, no significant association was observed regarding XPC rs2228000. Third, there was a synergistic influence of smoking and betel quid chewing behaviors and XPC rs2228001 genotype on oral cancer risk. Last, functional experiments showed DNA repair capacity was lower for AC/CC carriers than AA carriers. Conclusion: XPC rs2228001 C allele, which was associated with decreased DNA repair capacity, may interact with smoking and betel quid chewing behaviors on oral cancer risk.Oral cancer is the tenth most common cancer worldwide, and Taiwan has one of the highest incidences (1). Based on the most updated annual statistics from the government, oral cancer is of the fourth death-causing cancers among Taiwanese males (2). Uniquely, betel quid chewing, in addition to cigarrete smoking and alcohol drinking, has been identified as an effective environmental factor to oral cancer risk in Taiwan (3). The Taiwan government has embarked in population screen searching for the oral cancer candidates for early cure and medication to lower its incidence, however, the death rate and incidence of oral cancer were still high. Therefore, novel predictors for oral cancer risk are still needed.There are five major DNA repair systems, consisting of more than 130 genes, and teaming up to maintain the stability and integrity of the human genome. Among them, the nucleotide excision repair (NER) system is in charge of removing DNA crosslinks, bulky adducts, alkylating DNA adducts, oxidative DNA adducts and thymidine dimers (4-6). In NER machinery, four major steps (adduct recognition, lesion DNA incision, gapped DNA fulfilling and ligation) and several core players, including xeroderma pigmentosum complementation group C (XPC)-RAD23B, play critical roles as key enzymes (4, 5) Theoretically, subtle genetic 441 This article is freely accessible online.

Section: Methodsmentioning

confidence: 99%

Interaction of DNA Repair Gene XPC With Smoking and Betel Quid Chewing Behaviors of Oral Cancer

Cancer Genomics Proteomics

Shih

et al. 2021

Self Cite

“…Methodology for the determination of MMP9 rs3918242 genotype. DNA was extracted using QIAamp Blood Mini Kit (Blossom, Taipei, Taiwan) as previously described (37)(38)(39)(40), and MMP9 rs3918242 genotyping was conducted as per our previous practice (41,42). Briefly, the primer sequences for MMP9 rs3918242 genotyping were 5'-TGGTCAACGTAGTGAAACCCCATCT-3' and 5'-TCCAGCCCCAATTATCACACTTAT-3'.…”

Section: Methodsmentioning

confidence: 99%

The Association of MMP9 Promoter Rs3918242 Genotype With Gastric Cancer

Tsai

et al. 2021

Anticancer Res

Background/Aim: Matrix metalloproteinase 9 (MMP9) is highly expressed in gastric cancer but the role of MMP9 is unclear. This study aimed at revealing the association of MMP9 promoter rs3918242 genotypes with gastric cancer risk. Materials and Methods: MMP9 rs3918242 genotypes of 121 patients with gastric cancer and 363 healthy individuals were examined by polymerase chain reaction-restriction fragment length polymorphism methodology using serum samples. Results: MMP9 rs3918242 TT genotype carriers had an elevated gastric cancer risk compared to wild-type CC carriers (odds ratio=3.92, 95% confidence interval=1.28-11.99; p=0.0103). Patients with CT/TT genotypes were at higher risk of metastasis (p=0.0178) than those with CC. No correlation was found between MMP9 rs3918242 genotype and gastric cancer risk with smoking or alcohol behavior, nor Helicobacter pylori infection. No correlation was observed for MMP9 rs3918242 genotypic distributions with age, gender, or body mass index. Conclusion: Carrying a T allele for MMP9 rs3918242 may be predictive for higher gastric cancer risk, and as a predictor for higher risk of metastasis.

“…Genomic DNA from the blood of each participant was extracted, aliquoted and stored at −80˚C as routinely conducted (23)(24)(25). The MMP1 genotyping methodology was the same as in our recently published article (26).…”

Section: Methodology For Determination Of Mmp1 Rs1799750 Genotypementioning

confidence: 99%

Association of Matrix Metalloproteinase-1 Genotypes With Bladder Cancer Risk

Liao

Tsai

et al. 2021

In Vivo

Self Cite

Aim: There is very little literature reporting the association of matrix metalloproteinase-1 (MMP1) with personal susceptibility to bladder cancer. In the current study, we carried out the first examination of the contribution of MMP1 rs1799750 to bladder cancer risk in Taiwanese. Materials and Methods: A total of 375 bladder cancer cases and 375 healthy controls were genotyped for MMP1 rs1799750 via polymerase chain reaction-restriction fragment length polymorphism methodology and this was evaluated for association with clinicopathological factors. Results: The frequencies of MMP1 rs1799750 2G/2G, 1G/2G, and 1G/1G genotypes were 35.7%, 44.8% and 19.5% in the group with bladder cancer and 32.5%, 46.4%, and 21.1% in the healthy control group (p for trend=0.6362). The odds ratios (ORs) for bladder cancer risk after adjusting for age and gender for those carrying 1G/2G and 1G/1G genotypes at MMP1 rs1799750 were 0.88 p=0.3990), respectively, compared with the wild-type 2G/2G genotype. In allelic frequency analysis, the adjusted OR for those carrying the 1G allele at MMP1 rs1799750 was 0.87 (95% CI=0. 71-1.23, p=0.3479) compared to those people carrying a 2G allele. Conclusion: Our findings indicated that the genotypes at MMP1 rs1799750 appear to play little role in determining personal susceptibility to bladder cancer for Taiwanese.