Significant association of FcɛRIα promoter polymorphisms with aspirin-intolerant chronic urticaria

Two Different Transcription Factors Discriminate the −315C>T Polymorphism of theFcεRIα Gene: Binding of Sp1 to −315C and of a High Mobility Group-Related Molecule to −315T

“…6. During the preparation of this manuscript, Bae et al (5) reported that the transcription factor MAZ binds to Ϫ315C. Although we also found specific binding of a nuclear protein to the Ϫ315C probe in the present study, it was identified as Sp1 and not MAZ, based on the following findings: i) the specific band was supershifted by antiSp1 Ab, ii) recombinant Sp1 and in vitro-translated Sp1 caused the same band shift of the probe in EMSA (Fig.…”

“…3A, the target band marked with double asterisks was supershifted by addition of anti-Sp1 Ab but was not affected by addition of Ab against another Sp1-family protein, Sp3, and irrelevant anti-YY1 Ab. In addition, Ab against MAZ, which was recently reported by Bae et al (5), to bind the Ϫ315C allele probe exhibited no effect on this specific band (Fig. 3A).…”

“…The necessity of the ␣-chain in IgE-mediated allergic reactions was definitively proved by the abolishment of anaphylaxis in ␣-chain-deficient mice (1). Recently, several groups including us reported studies regarding the association of Ϫ315 and Ϫ66 SNPs in the ␣-chain promoter with allergic diseases (2)(3)(4)(5). In the present study, we analyzed the role of these SNPs in the function of the ␣-chain promoter and found that the Ϫ315C and Ϫ315T sequences are recognized by two different transcription factors, as summarized in Fig.…”

“…However, the relationship between Ϫ315 and Ϫ66 SNP is controversial, which may reflect statistical interdependence. In brief, Ϫ315 and Ϫ66 SNPs were described to be in complete linkage disequilibrium (LD) in one report (4) but not in LD in another one (5). Therefore, in the present study, we determined the genotypes of Ϫ315 and Ϫ66 in the Japanese population to examine the relationship of the two SNPs.…”

Histamine Release and Inflammatory Cell Infiltration in Airway Mucosa in Methylene Diphenyl Diisocyanate (MDI)-Induced Occupational Asthma

“…Samples were prepared to measure histamine release (HR) induced by the MDI-HSA conjugate and other stimuli according to methods described previously [14]. Briefly, 20 ml blood were obtained from each subject and mixed with 2.5 ml of 6% dextran-3% dextrose-physiologic saline and 1 ml of ethylenediamine tetra-acetic acid.…”