Significant association of <i><scp>PRMT</scp>6</i> hypomethylation with colorectal cancer

Pan, Ranran; Yu, Hang; Dai, Jie; Zhou, Cong; Ying, Xiuru; Zhong, Jie; Zhao, Jun; Zhang, Yihan; Wu, Boyi; Mao, Yiyi; Wu, Dongping; Ying, Jieer; Duan, Shiwei

doi:10.1002/jcla.22590

Cited by 10 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sodium bisulfite modification was performed using the EZ DNA Methylation‐Gold™ Kit (Zymo Research, Orange, CA, USA). DNA methylation was measured by quantitative methylation‐specific polymerase chain reaction (PCR) (qMSP), using a LightCycler 480 machine (Roche Diagnostics, Mannheim, Germany), as described in previous studies . The qMSP primer sequences for SHMT1 are shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…DNA methylation was measured by quantitative methylation-specific polymerase chain reaction (PCR) (qMSP), using a LightCycler 480 machine (Roche Diagnostics, Mannheim, Germany), as described in previous studies. 16,17 The qMSP primer sequences for SHMT1 are shown in Table 1. The reaction conditions were as follows: initial denaturation at 95°C for 60 seconds, followed by 45 cycles of denaturation at 95°C for 20 seconds, annealing at 58°C for 45 seconds, and extension at 72°C for 20 seconds.…”

Section: Sodium Bisulfite Modification Was Performed Using the Ez Dnamentioning

confidence: 99%

See 1 more Smart Citation

Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Wang

Ying

et al. 2018

Clinical Laboratory Analysis

Self Cite

View full text Add to dashboard Cite

Background Serine hydroxymethyltransferase 1 (SHMT1) is an enzyme involved in folic acid metabolism and is known to contribute to the development of hypertension. We evaluated the relationship between SHMT1 promoter methylation and essential hypertension (EH). Methods Quantitative methylation‐specific polymerase chain reaction was used to measure the SHMT1 promoter methylation level in 241 EH patients and 288 age‐ and gender‐matched healthy individuals. The diagnostic value of SHMT1 promoter hypermethylation was analyzed using a receiver operating characteristic (ROC) curve. The Gene Expression Omnibus (GEO) database and dual‐luciferase reporter assay were used to validate our findings. Results Compared with the control group, significant differences in SHMT1 promoter methylation were found in both EH and hyperhomocysteinemia groups (P < 0.001 and P = 0.029, respectively). The area under the curve of the diagnosis of SHMT1 promoter hypermethylation for EH was 0.808, with a sensitivity and specificity of 73.9% and 77.8%, respectively. The risk of SHMT1 promoter hypermethylation was significantly higher in the >65‐year group than in the ≤65‐year group (odds ratio = 3.925; 95% confidence interval = 2.141‐7.196). In addition, GEO database analysis showed that 5‐aza‐deoxycytidine increased gene expression in several carotid endothelial cell lines. A dual‐luciferase reporter assay revealed that the target sequence in the SHMT1 promoter upregulated gene expression. Conclusion Our findings indicate that SHMT1 promoter hypermethylation increases the risk of EH and may be a promising biomarker for EH.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Sodium Bisulfite Modification Was Performed Using the Ez Dnamentioning

confidence: 99%

Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Wang

Ying

et al. 2018

Clinical Laboratory Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…Amplification in the LightCycler 480 real-time PCR system (Roche Biochemical, Mannheim, Germany) was run under the following conditions: 5 min of activation PCR step at 95 °C, 45 cycles of annealing at 95 °C for 15 s, and extension at 60 °C for 60 s. Each plate included sample DNA, positive quality control, and water as blank. QMSP results were calculated using the percentage of methylated reference (PMR), which was described to represent the methylation level (Quillien et al 2012, Liu et al 2014, Pérez-Carbonell et al 2010, Andresen et al 2015, Pan et al 2018):…”

Section: Real-time Quantitative Methylation-specific Polymerase Chain...mentioning

confidence: 99%

Hypermethylation of BMPR2 and TGF-β Promoter Regions in Tibetan Patients with High-Altitude Polycythemia at Extreme Altitude

Zhaxi,

Gesang,

Huang

et al. 2024

Biochem Genet

View full text Add to dashboard Cite

Although the expression of many genes is associated with adaptation to high-altitude hypoxic environments, the role of epigenetics in the response to this harsh environmental stress is currently unclear. We explored whether abnormal DNA promoter methylation levels of six genes, namely, ABCA1, SOD2, AKT1, VEGFR2, TGF-β, and BMPR2, affect the occurrence and development of high-altitude polycythemia (HAPC) in Tibetans. The methylation levels of HAPC and the control group of 130 Tibetans from very high altitudes (> 4500 m) were examined using quantitative methylation-specific real-time PCR (QMSP). Depending on the type of data, the Pearson chi-square test, Wilcoxon rank-sum test, and Fisher exact test were used to assess the differences between the two groups. The correlation between the methylation levels of each gene and the hemoglobin content was explored using a linear mixed model. Our experiment revealed that the methylation levels of the TGF-β and BMPR2 genes differed significantly in the two groups (p < 0.05) and linear mixed model analysis showed that the correlation between the hemoglobin and methylation of ABCA1, TGF-β, and BMPR2 was statistically significant (p < 0.05). Our study suggests that levels of TGF-β and BMPR2 methylation are associated with the occurrence of HAPC in extreme-altitude Tibetan populations among 6 selected genes. Epigenetics may be involved in the pathogenesis of HAPC, and future experiments could combine gene and protein levels to verify the diagnostic value of TGF-β and BMPR2 methylation levels in HAPC.

show abstract

“…PRMT6 levels are elevated in colon cancer possibly due to the hypomethylation of PRMT6 promoter regions [ 177 , 178 ]. An abundance of PRMT6 is correlated with the shorter disease-free survival of colon cancer patients [ 178 ].…”

Section: Role Of Prmt6 In Cancersmentioning

confidence: 99%

Structure, Activity and Function of the Protein Arginine Methyltransferase 6

Gupta

Kadumuri

Singh

et al. 2021

Life

View full text Add to dashboard Cite

Members of the protein arginine methyltransferase (PRMT) family methylate the arginine residue(s) of several proteins and regulate a broad spectrum of cellular functions. Protein arginine methyltransferase 6 (PRMT6) is a type I PRMT that asymmetrically dimethylates the arginine residues of numerous substrate proteins. PRMT6 introduces asymmetric dimethylation modification in the histone 3 at arginine 2 (H3R2me2a) and facilitates epigenetic regulation of global gene expression. In addition to histones, PRMT6 methylates a wide range of cellular proteins and regulates their functions. Here, we discuss (i) the biochemical aspects of enzyme kinetics, (ii) the structural features of PRMT6 and (iii) the diverse functional outcomes of PRMT6 mediated arginine methylation. Finally, we highlight how dysregulation of PRMT6 is implicated in various types of cancers and response to viral infections.

show abstract

Significant association of PRMT6 hypomethylation with colorectal cancer

Abstract: Our study firstly indicated that the hypomethylation of PRMT6 promoter could be a novel diagnostic biomarker for CRC.

Cited by 10 publications

References 46 publications

Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Hypermethylation of BMPR2 and TGF-β Promoter Regions in Tibetan Patients with High-Altitude Polycythemia at Extreme Altitude

Structure, Activity and Function of the Protein Arginine Methyltransferase 6

Contact Info

Product

Resources

About