Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria

Szegedi, Andrea; Irinyi, Beatrix; Gál, Mónika; Hunyadi, J.; Dankó, Katalin; Kiss, Emese; Sipka, Sándor; Szegedi, Gyula; Gyimesi, Edit

doi:10.1111/j.1365-2133.2006.07205.x

Cited by 57 publications

(63 citation statements)

References 35 publications

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“…Our study is one of the first to compare CD63 and CD203c expression in a group of CU patients. Based on experience of other authors, who demonstrated that atopic donor basophils showed marked upregulation of CD63 and CD203c molecules after stimulation with serum from CU patients in this study we used highly atopic donor basophils [12,14,15]. Our study confirmed that serum from patients with CU, but not control sera, on average significantly upregulated atopic donor basophil CD63 and CD203c expression.…”

Section: Discussionsupporting

confidence: 81%

“…After ROC curve analysis, we concluded that CD203c did not have sufficient diagnostic power. Other authors, who tested CD63 [11][12][13][14][15] or CD203c expression [17] in CU patients, found significant correlation between ASST and both basophil activation tests positivity. The discrepancy between CD63 and CD203c correlations with ASST in our study can reflect either different mechanism of CD63 and CD203c molecules upregulation or differences in methodology of tests.…”

Section: Discussionmentioning

confidence: 92%

“…Recently, the ability of CU patient's serum to evoke expression of CD63 and CD203c on donor human basophils assessed by flow cytometry has been tested. There are a few published studies assessing serum-induced CD63 expression measurement as a diagnostic tool in CAU [11][12][13][14][15][16] and only 2 studies done by measured CD203c expression [16,17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Serum induced CD63 and CD203c activation tests in chronic urticaria

Chomičienė¹,

Jurgauskienė²,

Kowalski

et al. 2014

Open Medicine

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serum induced CD63 and CD203c activation tests in chronic urticaria

Chomičienė¹,

Jurgauskienė²,

Kowalski

et al. 2014

Open Medicine

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show abstract

“…One of them, the gold standard, is the basophil histamine release (HR) test [18]. In recent studies, a new method, the modified CD63 expression assay, was found to be a reliable method for the diagnosis of AIU [19,20,21,22]. …”

Section: Introductionmentioning

confidence: 99%

Clinical and Laboratory Examinations in the Subgroups of Chronic Urticaria

Irinyi

Széles

Gyimesi

et al. 2007

Int Arch Allergy Immunol

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Background: The aetiology of chronic urticaria is heterogeneous. Physical urticaria (PU) is estimated at around 35%, autoimmune urticaria (AIU) at 25% and chronic idiopathic urticaria (CIU) at 35% of all chronic urticaria cases. Methods: Differences in clinical and laboratory parameters among AIU, PU and CIU groups were examined. AIU was diagnosed if the basophil CD63 assay was positive. Demographic data, severity of symptoms and association with allergic and autoimmune diseases were analysed by the aid of a questionnaire. Immunoassays were carried out and the effectiveness of therapy was also investigated. Results: Concerning the urticaria score, AIU patients had significantly higher total urticaria scores than patients with CIU (p = 0.013), dermatographic urticaria (p = 0.05) or cholinergic urticaria (p = 0.038). Between CIU and dermatographic urticaria and between CIU and cholinergic urticaria patients, we found insignificant differences in the urticaria score (p = 0.707 and p = 0.336, respectively). AIU was more frequently associated with autoimmune diseases in the personal history (p < 0.001) and with other types of urticaria in the family history (p < 0.001). Also, anti-thyroid antibodies were more frequently detected in the AIU group. Antihistamine therapy was less effective in the AIU group (12.8%) than in the PU (70.3%) and CIU groups (68.6%), but there were no significant differences between the CIU and PU groups regarding the effectiveness of antihistamine therapy. Conclusion: The autoimmune subgroup represents the most severe form of chronic urticaria. On the other hand, there were no significant differences between the CIU and PU groups neither in urticaria scores nor in response to antihistamine therapy.

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“…The basophil histamine release assay (HRA) is currently the "gold standard" for detecting functional antibodies in the serum of patients with CU [5][6][7]. Improved screening tests have been sought in the last decade; for instance, flow cytometry used to determine the ability of a CU patient's serum to evoke an expression of CD63 and CD203c on donor basophils is showing some promise [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%