Significant Decline of Peripheral Myeloid Dendritic Cells Following Multiple Trauma

Henrich, Dirk; Maier, M.; Relja, Borna; Trendafilov, Petar; Schiessling, Serin; Wirth, Manuela; Ottilinger, Thorsten; Nielsen, Ann-Kathrin; Wyen, H.; Marzi, Ingo

doi:10.1016/j.jss.2008.06.038

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…A similar time scheme has been observed with other markers (for example, C-reactive protein, procalcitonin, and lipopolysaccharide-stimulated interleukin-1β synthesis) of inflammation during the posttraumatic inflammatory response (17,18). Moreover, a traumaassociated differential activation of DC subtypes with regard to the surface expression of HLA-DR can be found with enhanced expression on MDCs in contrast to PDCs.…”

Section: Discussionsupporting

confidence: 73%

“…This leads to different migratory properties and individual capacity to recruit different cell types at the site of inflammation (14). In a recent study we showed that the clinical impact of trauma leads to a decreasing number of MDCs whereas PDCs are not affected, which seems to be related to increased apoptosis of this cell type (17,19). Therefore the results of this study are presented with the analysis of the two subtypes-MDCs and PDCs.…”

Section: Discussionmentioning

confidence: 89%

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Platelet Factor 4 Is Highly Upregulated in Dendritic Cells after Severe Trauma

Maier

Geiger

Henrich

et al. 2009

Mol Med

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Dendritic cells (DCs) represent an important linkage between the innate and adaptive immune system and express proinflammatory transcriptomic products early after trauma. The use of a genomic approach recently revealed that platelet factor 4 (PF4) is significantly upregulated in DCs in patients after multiple trauma. However, knowledge about subsequent PF4 alteration and its potential clinical relevance in the context of multiple trauma is still limited. We used quantitative reverse transcription-polymerase chain reaction to analyze PF4 expression in both myeloid DCs (MDCs) and plasmocytoid DCs (PDCs) isolated from 10 patients after multiple trauma. Intracellular PF4 as well as HLA-DR expression were detected by flow cytometry. Furthermore, DCs and peripheral blood mononuclear cells were incubated on a monolayer of human umbilical endothelial cells and their adhesion properties were analyzed. The ratio of the DC subtypes (MDC and PDC) was assessed by flow cytometry. PF4 expression significantly increased on d 1 and d 2 as measured by reverse transcription-polymerase chain reaction. Intracellular PF4 content in MDCs and PDCs was significantly elevated in trauma patients compared with healthy controls. In addition, the surface antigen HLA-DR on MDCs was significantly elevated on d 1 and d 4 after trauma in patients compared with controls. However, cell adhesion of DCs did not show any significant differences between patients and controls. PF4 concentration in MDCs and PDCs significantly correlated with the injury severity score. These results confirm an early and subsequent posttraumatic activation of PF4 in DCs. PF4 also participates in the posttraumatic activation of DCs in relation to injury severity, a role that might be preferably based on the modification of receptor expression, whereas adhesion properties are largely unaffected.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 89%

Platelet Factor 4 Is Highly Upregulated in Dendritic Cells after Severe Trauma

Maier

Geiger

Henrich

et al. 2009

Mol Med

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“…Obviously MDCs have a shorter life span and higher turnover rate and are more sensitive to stress compared with PDC or T-cells [21]. Recently we showed that the number of MDC in relation to PDC is reduced after severe trauma [8]. According to the present study findings, apoptosis is significantly increased in MDC but not in PDC, which might explain the observation of MDC loss in the latter study.…”

Section: Different Apoptosis In MDC and Pdcsupporting

confidence: 52%

“…So far we have seen that severe trauma differentially influences the DC subtypes and leads to a significant upregulation of Bcl-2 initially after trauma [8,9].…”

Section: Apoptosis In Sepsis/trauma Hemorrhagementioning

confidence: 99%