Significant difference of the culturable anaerobic microbial species in GujingTribute pitmud with different ages

Li, Junwei; He, Hongkui; Cao, Runjie; Li, Anjun; Zhang, Zhizhou

doi:10.1088/1757-899x/394/2/022025

Cited by 1 publication

(1 citation statement)

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“…By annotating the sequence information of gene ME2 in NCBI, Ruminococcaceae bacterium CPB6 which is prevalent in pit mud, especially in aged pit mud [ 4 , 21 ], has the highest similarity. Its 16S rRNA sequence information was input into GRPWREC to predict the optimal screening medium, and the result showed that the optimal medium was DSMZ_330.…”

Section: Resultsmentioning

confidence: 99%

Study on the Effect of Key Genes ME2 and adhE during Luzhou-Flavor Baijiu Brewing

Zhou

Xia

Zhao

et al. 2022

Foods

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Luzhou-flavor baijiu (LFB) is brewed by the combined action of various microorganisms, and its flavor is affected by the microbial community and the genes they express, but which genes are the key ones during LFB brewing is less clear. Based on our previous studies the genes ME2 and adhE were identified as key genes, but which role they play was also unknown. In this study functional microorganisms were screened based on the key genes ME2 and adhE, and they were identified to be Rummeliibacillus suwonensis, Clostridium tyrobutyricum and Lactobacillus buchneri. Then simulated fermentation experiments were carried out with the functional microorganisms, and during the fermentation process expression of the key genes and the amounts of the main flavors were detected to analyze the role of the key genes. The results showed that the key gene ME2 was significantly positively correlated with the contents of the main acids, however the key gene adhE and the formation of the main esters in the LFB brewing process was a significant positive correlation. This study verified the two key genes ME2 and adhE complement each other in the LFB brewing process, playing an important role in promoting the formation of flavor substances, and are very beneficial to improve the quality of LFB.

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Section: Resultsmentioning

confidence: 99%