Significant dissociation of expression patterns of the basic helix–loop–helix transcription factors <i>Dec1</i> and <i>Dec2</i> in rat kidney

Wu, Tao; Ota, Tsuguhito; Zhuge, Fen; Sun, Lu; Xu, Bin; Kato, Harubumi

doi:10.1242/jeb.052100

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…We found that DRF can induce a bimodal temporal pattern of corticosterone secretion in normal rats, with the first peak appearing at the beginning of the day, depending on the feeding time, and the second one appearing just before the LD transition controlled by the SCN clock (43), which was in good agreement with previous observations in normal mice under the DRF condition (22). These observations suggest the secretion of glucocorticoid hormone is under the control of both internal SCN and external feeding schedule in normal rodents.…”

Section: Discussionmentioning

confidence: 79%

Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats

Zhuge

Sun

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats. Am J Physiol Endocrinol Metab 302: E1027-E1035, 2012. First published February 7, 2011 doi:10.1152/ajpendo.00651.2011There is increasing awareness of the link between impaired circadian clocks and multiple metabolic diseases. However, the impairment of the circadian clock by type 2 diabetes has not been fully elucidated. To understand whether and how the function of circadian clock is impaired under the diabetic condition, we examined not only the expression of circadian genes in the heart and pineal gland but also the behavioral rhythm of type 2 diabetic and control rats in both the nighttime restricted feeding (NRF) and daytime restricted feeding (DRF) conditions. In the NRF condition, the circadian expression of clock genes in the heart and pineal gland was conserved in the diabetic rats, being similar to that in the control rats. DRF shifted the circadian phases of peripheral clock genes more efficiently in the diabetic rats than those in the control rats. Moreover, the activity rhythm of rats in the diabetic group was completely shifted from the dark phase to the light phase after 5 days of DRF treatment, whereas the activity rhythm of rats in the control group was still under the control of the suprachiasmatic nucleus (SCN) after the same DRF treatment. Furthermore, the serum glucose rhythm of type 2 diabetic rats was also shifted and controlled by the external feeding schedule, ignoring the SCN rhythm. Therefore, DRF shows stronger effect on the reentrainment of circadian rhythm in the type 2 diabetic rats, suggesting that the circadian system in diabetes is unstable and more easily shifted by feeding stimuli. daytime restricted feeding; Clock genes; type 2 diabetes; pineal gland; heart LIFE ON EARTH IS GOVERNED by the 24-h light-dark (LD) environmental cycle. Numerous physiological processes such as sleep-wake cycle, behavior activity, cardiovascular function, etc., are coordinated to this 24-h cycle by an internal circadian timing system that is comprised of a hierarchy of circadian clocks (11,25,45). The mechanism underlying circadian clocks is composed of a set of interlocking transcription/translation feedback loops involving multiple clock genes [Clock, Bmal1, Per(s), Cry(s), Dec(s)] and their proteins (23, 28). These circadian clocks reside not only in the hypothalamic suprachiasmatic nucleus (SCN), which is recognized as the master clock, but also in almost all peripheral tissues, including liver, heart, and pineal gland (28,30,44).Malfunction of the biological clock is commonly present in our daily life. The modern lifestyle, including shift work, nocturnal activity, and late-night dietary, easily causes internal dyschronization. There is increasing awareness of the link between impaired circadian clocks and multiple metabolic diseases (26,33). It has been demonstrated that Clock mutant mice have an attenuated feeding rhythm and develop metabolic syndrome, including hyperglycemia...

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Section: Discussionmentioning

confidence: 79%

Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats

Zhuge

Sun

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

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“…Gene expression of both Dec1 and Dec2 exhibits a circadian pattern of expression in the rat heart while only Dec2 shows a comparable rhythm in the kidney (Wu et al 2011). Since rats are nocturnal, they normally eat at night.…”

Section: The Kidney Clockmentioning

confidence: 99%

The Circadian Clock in the Regulation of Renal Rhythms

Solocinski

Gumz

2015

J Biol Rhythms

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Since the kidney is integral to maintenance of fluid and ion homeostasis, and therefore blood pressure regulation, its proper function is paramount. Circadian fluctuations in blood pressure, renal blood flow, glomerular filtration rate, and sodium and water excretion have been documented for decades, if not longer. Recent studies on the role of circadian clock proteins in the regulation of a variety of renal transport genes suggest that the molecular clock in the kidney controls circadian fluctuations in renal function. The circadian clock appears to be a critical regulator of renal function with important implications for the treatment of renal pathologies which include chronic kidney disease and hypertension. The development, regulation, and mechanism of the kidney clock are reviewed here.

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“…DEC2 mRNA is known to show a rhythmic expression in SCN, kidneys, liver, muscles, sarcoma cells and hepatocellular carcinoma HepG2 cells (3,21,25,38,39). However, there are no studies available on the rhythmic expression of DEC2 protein.…”

Section: Discussionmentioning

confidence: 99%

Rhythmic expression of DEC2 protein in vitro and in vivo

et al. 2016

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Basic helix-loop-helix (bHLH) transcription factor DEC2 (bHLHE41/Sharp1) is one of the clock genes that show a circadian rhythm in various tissues. DEC2 regulates differentiation, sleep length, tumor cell invasion and apoptosis. Although studies have been conducted on the rhythmic expression of DEC2 mRNA in various tissues, the precise molecular mechanism of DEC2 expression is poorly understood. In the present study, we examined whether DEC2 protein had a rhythmic expression. Western blot analysis for DEC2 protein revealed a rhythmic expression in mouse liver, lung and muscle and in MCF-7 and U2OS cells. In addition, AMP-activated protein kinase (AMPK) activity (phosphorylation of AMPK) in mouse embryonic fibroblasts (MEFs) exhibited a rhythmic expression under the condition of medium change or glucose-depleted medium. However, the rhythmic expression of DEC2 in MEF gradually decreased in time under these conditions. The medium change affected the levels of DEC2 protein and phosphorylation of AMPK. In addition, the levels of DEC2 protein showed a rhythmic expression and in MCF-7 and U2OS cells. The results showed that the phosphorylation of AMPK immunoreactivity was strongly detected in the liver and lung of knockout mice compared with that of wild-type mice. These results may provide new insights into rhythmic expression and the regulation between DEC2 protein and AMPK activity.

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Significant dissociation of expression patterns of the basic helix–loop–helix transcription factors Dec1 and Dec2 in rat kidney

Cited by 9 publications

References 39 publications

Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats

Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats

The Circadian Clock in the Regulation of Renal Rhythms

Rhythmic expression of DEC2 protein in vitro and in vivo

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