Significant dominance of fibrinogen over immunoglobulins, C‐reactive protein, cholesterol and triglycerides in maintaining increased red blood cell adhesiveness/aggregation in the peripheral venous blood: a model in hypercholesterolaemic patients

Schechner, Vered; Shapira, Itzhak; Berliner, Shlomo; Comaneshter, Doron; Hershcovici, Tiberiu; Orlin, Jerome; Zeltser, David; Rozenblat, Meirav; Lachmi, K.; Hirsch, Madelyn J.; Beigel, Yitzhak

doi:10.1046/j.1365-2362.2003.01260.x

Cited by 43 publications

(44 citation statements)

References 23 publications

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“…We found that neither of these candidates were significant contributors to the phenomenon, as have been found previously. 4,33,34 Consequently, the milieu of inflammation-sensitive proteins and adhesive macromolecules, specifically fibrinogen, has a dominant role in maintaining the pathological RBC aggregation found in obese individuals and described in the present study.…”

Section: Discussionsupporting

confidence: 51%

“…Specifically, obesity is accompanied by increased circulating CRP and fibrinogen concentrations. 4,17,18,30 The latter is a well-characterized RBC aggregation inducer, [31][32][33][34][35][36] either through a nonspecific mechanism 31,32 or by specific binding of fibrinogen to RBC surface membranes, 36 and thus bridging between the cells. Furthermore, CRP has been shown to correlate with indexes of RBC aggregation as well.…”

Section: Discussionmentioning

confidence: 99%

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Flow-resistant red blood cell aggregation in morbid obesity

et al. 2004

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OBJECTIVE:Enhanced red blood cell (RBC) aggregation has an adverse effect on microcirculatory blood flow and tissue oxygenation. It has been previously shown that obesity is associated with increased RBC aggregation. The objectives of the present study were to further characterize obesity-related RBC aggregation and to examine whether the enhanced aggregation is a plasma-or cellular-dependent process. METHODS: Obese (body mass index (BMI) ¼ 4076.3 kg/m 2 , n ¼ 22) and nonobese (BMI ¼ 2473.4 kg/m 2 , n ¼ 18) individuals were evaluated for inflammation markers and aggregation parameters. Aggregation parameters were derived from the distribution of RBC population into aggregate sizes, and from the variation of the distribution as a function of flow-derived shear stress, using a cell flow properties analyzer. To differentiate plasmatic from cellular factors, we determined the aggregation in the presence of autologous plasma or dextran-500kDa and calculated the plasma factor (PF) in the obese group. PF ranges from 0 to 1. When the PF ¼ 1, the aggregation is all due to plasmatic factors, when PF ¼ 0, the altered aggregation depends entirely on cellular factors, whereas 0oPFo1 reflects the joint contribution of cellular and plasmatic factors. RESULTS: Obese subjects had relatively larger aggregates that were more resistant to dispersion by flow. The calculated PF in the obese group was 0.9, indicating a pronounced contribution of plasma to RBC aggregation in obesity. DISCUSSION: Our results suggest that obese individuals present pathological plasma-dependent RBC aggregation, which is probably triggered by plasma macromolecules associated with the inflammatory response. These findings impact the future attempts to develop strategies aimed at attenuation of the enhanced RBC aggregation in obese individuals.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Flow-resistant red blood cell aggregation in morbid obesity

et al. 2004

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“…RBC aggregation occurs only in the presence of macromolecules (i.e., blood proteins) and depends on their concentration and type. 29,30 Aggregation also occurs in the presence of neutral macromolecules. 31,32 In our work, we focused on accessing the influence of media factors in the aggregation of RBCs.…”

Section: Factors Affecting Red Blood Cell Aggregationmentioning

confidence: 99%

“…14,29 Among blood plasma proteins (macromolecules) affecting RBC aggregation, the main role is attributed to fibrinogen. Schechner et al 30 showed that fibrinogen is the most significant determinant of RBC aggregation. As fibrinogen is also a determinant of the blood coagulation process, it might be considered the most significant protein for blood circulation.…”

Section: Factors Affecting Red Blood Cell Aggregationmentioning

confidence: 99%

Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

et al. 2016

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Abstract. Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

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“…The plasma fibrinogen (Fib) molecule participates in fibrin formation, red blood cells (RBCs) aggregation and when at high concentrations is known as an inflammatory acute phase protein [1][2][3]. Fibrinogen binding to erythrocyte CD47 receptor protein belonging to Rh complex that stablish contact with 4.2 cytoskeletal protein which interact with band 3 protein [4,15].…”

Section: Introductionmentioning

confidence: 99%

Fibrinogen Effects of Erythrocyte Nitric Oxide Metabolism in Presence of Timolol

Saldanha¹,

Freitas²,

Silva-Herdade³

2018

Clin Med Invest

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Timolol inhibits erythrocyte membrane acetylcholinesterase (AChE) enzyme activity originates less active enzyme complex forms unable to change the normal values of the nitric oxide (NO) efflux from erythrocytes. The active complex resulting from acetylcholine (ACh) binding to AChE induces higher values of NO efflux. Increased values of NO efflux were also observed in presence of high fibrinogen (Fib) concentration with return to normal levels of NO efflux when ACh is added. Association between NO efflux and erythrocyte deformability values has been evidenced.The objective of this study was to evaluate the effects of high fibrinogen concentration on the erythrocyte NO metabolism and deformability in presence of timolol without and plus adenylyl cyclase or plus guanylyl cyclase inhibitors.Blood samples from healthy donors were divided in aliquots without (control) or with Fib or Fib plus timolol in absence or presence of adenylyl cyclase inhibitor (MDL) or guanylyl cyclase inhibitor (Ly). Erythrocyte deformability (ED), NO efflux, S-nitrosoglutathione (GSNO), nitrite and nitrate values were evaluated by referenced methods. In conclusion fibrinogen plus timolol increased ED at high shear stress without or with MDL. Fibrinogen increased NO efflux from erythrocytes and GSNO, nitrite and nitrate levels in presence of timolol plus guanylyl cyclase inhibitor.

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Significant dominance of fibrinogen over immunoglobulins, C‐reactive protein, cholesterol and triglycerides in maintaining increased red blood cell adhesiveness/aggregation in the peripheral venous blood: a model in hypercholesterolaemic patients

Cited by 43 publications

References 23 publications

Flow-resistant red blood cell aggregation in morbid obesity

Flow-resistant red blood cell aggregation in morbid obesity

Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Fibrinogen Effects of Erythrocyte Nitric Oxide Metabolism in Presence of Timolol

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