Significant Elevation of Survivin and Livin Expression in Human Colorectal Cancer: Inverse Correlation between Expression and Overall Survival

Ren, Xi; Biao, Wang Shou; Gang, Zhang Zhi

doi:10.1159/000331132

Cited by 30 publications

(35 citation statements)

References 25 publications

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“…It has been demonstrated to be expressed in transformed cells and multiple types of malignant tumor, including neuroblastoma (20) as well as carcinomas of the bladder (21,22), lung (23), nasopharynx (24), kidney (25), liver (26), colon/rectum (27), skin (28), bone (29) and stomach (30).…”

Section: Discussionmentioning

confidence: 99%

“…Livin, a novel member of the inhibitor of apoptosis (IAP) protein family, is not detected in the majority of normal tissues, however, is highly expressed in various types of human malignancy (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Increased activity of this protein may be used as a reliable prognostic factor for initial and late resistance to chemotherapeutic drugs in certain types of human tumor (31)(32)(33)(34)(35)(36)(37).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Shen

et al. 2012

Molecular Medicine Reports

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Abstract. Livin is a novel member of the inhibitor of apoptosis protein family that has been reported to be overexpressed in various types of human malignancy. Although several studies have demonstrated that livin may be used as an effective target for tumor therapy, few studies have investigated its role in human lung adenocarcinoma. In the present study, two different methods were used in order to investigate the tumor-suppressing effect of livin in human lung adenocarcinoma cells. Firstly, small interfering (si)RNA technology was used to down regulate livin expression; siRNA-mediated knockdown of livin was confirmed using reverse transcription quantitative polymerase chain reaction and western blot analysis, and cell proliferations was assessed using an MTT assay in vitro. Secondly, inhibition of livin expression was induced through the synergistic inhibitory effect between flavopiridol and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Experimental results revealed that, following transfection of the livin gene-silencing vector, the gene expression of livin was markedly decreased, SPC-A1 cell proliferation was significantly reduced and the therapeutic effect of the chemotherapy drug cisplatin was markedly improved. This growth inhibitory effect was also observed in the flavopiridol and TRAIL combination treatment group. In the flavopiridol and TRAIL combination treatment group, the protein expression of livin was significantly reduced and the survival rate of SPC-A1 cells was significantly lower than the flavopiridol and TRAIL single operation group. In conclusion, the RNA silencing and the synergistic inhibitory effect between flavopiridol with TRAIL was able to effectively inhibit the expression of livin, significantly decrease SPC-A1 tumor cell proliferation and significantly enhance sensitivity to the chemotherapy drug cisplatin. These findings suggest that livin may be used as a novel target for tumor gene therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Shen

et al. 2012

Molecular Medicine Reports

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“…Hernandez et al (17) confirmed the presence of this protein in 93% of patients with colon cancer, while Xi et al (18) observed its expression in 60.7% of CRC patients. Survivin has different functions depending on its location.…”

Section: Survivin (Pg/ml) -------------------------------------------mentioning

confidence: 96%

Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients

et al. 2016

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Abstract. Survivin is one of the apoptosis-related inhibitors that is associated with a more aggressive behavior and a poor prognosis in numerous types of malignancies, including colorectal cancer (CRC). The objective of the present study was to perform immunohistochemical tissue analysis of survivin expression and serum analysis of survivin levels in CRC patients. The study group consisted of 55 CRC patients. Survivin expression was assessed by immunohistochemistry in 38 patients using monoclonal antibodies. Color reactions were observed in the nucleus and cytoplasm of the cancer cells. The expression was defined based on the H-score method. The level of survivin was determined using the enzyme-linked immunosorbent assay method. A positive immunoreaction was observed in the tumor tissues of 84.2% (32/38) of patients with CRC, consisting of nuclear (63.2%; 24/38) and cytoplasmic (81.6%; 31/38) expression. The survivin nuclear expression was associated with tumor mass location and the presence of distant metastases (P=0.048 and P=0.026, respectively). Survivin was detected in the sera of 38.2% (21/55) of CRC patients and in 81.8% (18/22) of healthy individuals. Serum protein levels were found to correlate with hematocrit (P=0.035), hemoglobin (P=0.008) and albumin (P=0.045), but not with any of the investigated clinicopathological parameters. The immunohistochemical positive reaction of survivin in the nuclei of cancer cells may condition their proliferative capacity, which is associated with higher risk of developing metastatic foci. Thus, the present study suggests that the expression of survivin may have diagnostic implications in cancer of the colon and thus requires further research. By contrast, the survivin serum level in CRC patients appears to be diagnostically ineffectual for clinical use.

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“…Previous studies have demonstrated that the expression level of BIRC7 is significantly up-regulated in colorectal cancer, and negative in normal colonic mucosa, and univariate survival analysis have shown that the expression level of BIRC7 is negatively correlated with survival time [35].The positive expression rate of BIRC7 in patients with advanced neuroblastoma is significantly higher than that in patients at early or intermediate stage [36]. In this studies, we observed that overexpressions of BIRC7, in both SC/ASC and AC, were significantly associated with large tumor size, high TNM stage, lymph node metastasis, invasion and poor survival.These results might suggest that BIRC7 is a tumor maker for reflecting the carcinogenesis, progression , biological behaviors and prognosis in both SC/ASC and AC of gallbladder.…”

Section: Discussionmentioning

confidence: 99%

A Comparative study of clinicopathological significance, BIRC7, and STC2 expression between squamous cell/adenosquamous carcinomas and adenocarcinoma of gallbladder

Yuan¹,

Yang²,

Zou³

et al. 2014

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Gallbladder cancers (GBCs) are uncommon, but highly aggressive cancers. The majority of GBCs are adenocarcinomas (ACs), but rare subtypes of GBCs such as squamous cell carcinoma (SC) and adenosquamous carcinoma (ASC) are observed as well. The clinicopathological characteristics of SC/ASC have not been well documented. Expressions of BIRC7 and STC2 were observed in some tumors. However, BIRC7 and STC2 expressions and clinical significances in gallbladder cancer have not been reported.In this study, the protein expressions of BIRC7 and STC2 in 46 SCs/ASCs and 80 ACs were measured using immunohistochemistry.We demonstrated that positive BIRC7 and STC2 expressions were significantly associated with large tumor mass (>3cm), high TNM stage and lymph node metastasis in SC/ASC and AC (p<0.05). Positive expression of BIRC7 was significantly associated with invasion of around tissues and organs in both SC/ASC and AC. Additionally, negative BIRC7 and STC2 expressions were significantly associated with surgical curability in AC. Univariate Kaplan-Meier analysis showed that BIRC7 and STC2 expressions, differentiation, tumor size, TNM stages, invasion, lymph node metastasis, and surgical curability were significantly associated with post-operative survival in both SC/ASC and AC patients(p < 0.001). Multivariate Cox regression analysis showed that positive BIRC7 and STC2 expressions are independent poor-prognostic factors in both SC/ASC and AC patients.Our study suggested that positive BIRC7 and STC2 expressions are closely correlated with clinical, pathological, and biological behaviors as well as poor-prognosis of gallbladder cancer.

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Significant Elevation of Survivin and Livin Expression in Human Colorectal Cancer: Inverse Correlation between Expression and Overall Survival

Cited by 30 publications

References 25 publications

Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients

A Comparative study of clinicopathological significance, BIRC7, and STC2 expression between squamous cell/adenosquamous carcinomas and adenocarcinoma of gallbladder

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