Significant enhancement of nitrous oxide energy yields from wastewater achieved by bioaugmentation with a recombinant strain of Pseudomonas aeruginosa

Lin, Ziyu; Sun, Dezhi; Dang, Yan; Holmes, Dawn E.

doi:10.1038/s41598-018-30326-8

Cited by 19 publications

(5 citation statements)

References 46 publications

(49 reference statements)

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“…Such classifications may enable identification of the conditions required for a key mixed microbial community or an individual microbe to be enriched (Cases I and II Figure 5), ultimately allowing the development of an innovative microbial process. Accordingly, implementing bioaugmentation strategies (Laocharoen et al, 2015;Lin et al, 2018) or retrofitting treatment processes for valuable product biosynthesis, analogous to the concept of bioaugmentation technology where nitrifying bacteria are enriched in a tank installed in a sludge recycle line (Salem et al, 2003), are potential applications of this screening process (Figure 5). These findings will also enable research toward isolation or selective enrichment of promising microbes using suitable environmental conditions identified from metagenomic data.…”

Section: Metagenomic Screening Of Promising Bacteria Capable Of Producing Valuable Productsmentioning

confidence: 99%

Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis

et al. 2021

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Upcycling wastes into valuable products by mixed microbial communities has recently received considerable attention. Sustainable production of high-value substances from one-carbon (C1) compounds, e.g., methanol supplemented as an external electron donor in bioreactors for wastewater treatment, is a promising application of upcycling. This study undertook a gene-centric approach to screen valuable production potentials from mixed culture biomass, removing organic carbon and nitrogen from landfill leachate. To this end, the microbial community of the activated sludge from a landfill leachate treatment plant and its metabolic potential for the production of seven valuable products were investigated. The DNA extracted from the activated sludge was subjected to shotgun metagenome sequencing to analyze the microbial taxonomy and functions associated with producing the seven products. The functional analysis confirmed that the activated sludge could produce six of the valuable products, ectoine, polyhydroxybutyrate (PHB), zeaxanthin, astaxanthin, acetoin, and 2,3-butanediol. Quantification of the detected functional gene hit numbers for these valuable products as a primary trial identified a potential rate-limiting metabolic pathway, e.g., conversion of L-2,4-diaminobutyrate into N-γ-acetyl-L2,4,-diaminobutyrate during the ectoine biosynthesis. Overall, this study demonstrated that primary screening by the proposed gene-centric approach can be used to evaluate the potential for the production of valuable products using mixed culture or single microbe in engineered systems. The proposed approach can be expanded to sites where water purification is highly required, but resource recovery, or upcycling has not been implemented.

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Section: Metagenomic Screening Of Promising Bacteria Capable Of Producing Valuable Productsmentioning

confidence: 99%

Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis

et al. 2021

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“…Some denitrifiers reportedly lack the nosZ gene that encodes N 2 O reductase . For example, nearly 40% of 652 microbial genomes possessing NirK (nitrite reductase-encoding genes) lack nosZ . , Some denitrifying bacteria from the genera Pseudoxanthomonas , Stenotrophomonas , Luteimonas , Corynebacterium , and Moraxella were reported to have a truncated denitritation pathway, and their chromosomes lack nosZ . Even complete denitrifiers may downregulate nosZ expression under some conditions, e.g., when NO 3 – is replete while Cu is depleted. , Indeed, a very high prevalence of denitrifiers with truncated denitrification pathways was observed in a CANDO reactor .…”

Section: Challenges and Perspectives For N2o Utilizationmentioning

confidence: 99%

Recovery of Nitrous Oxide from Wastewater Treatment: Current Status and Perspectives

Duan¹,

Zhao²,

Koch

et al. 2020

ACS EST Water

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Nitrous oxide (N 2 O) has been studied intensively in wastewater treatment as a detrimental greenhouse gas. However, increasingly more studies have adopted a contrasting objective, recovering N 2 O from wastewater as an energy resource. This article critically reviewed and analyzed the current status of N 2 O recovery research in wastewater treatment, to identify knowledge gaps and guide future research. Overall, N 2 O recovery is a promising research direction while still in active development. At present, unstable nitritation, the low energy potential, and potential environmental risks of N 2 O harvesting render the recovery of N 2 O from mainstream wastewater technically and economically challenging. High-strength wastewater treatment is more favorable for N 2 O recovery due to the high energy potential, established nitritation approaches, and significant carbon/aeration savings. The coupled aerobic−anoxic nitrous decomposition operation (CANDO) process is currently the most investigated and promising N 2 O recovery process. Nevertheless, more research is needed for its implementation on a large scale. Research opportunities for the CANDO process have been identified in this paper. Meanwhile, N 2 O recovery via autotrophic denitritation is a more recent concept, with limited studies hitherto. More experiments are needed to investigate its technological feasibility. Furthermore, other novel N 2 O recovery processes, e.g., truncated denitrification and chemical oxidation, should also be explored to facilitate the recovery of N 2 O from wastewater.

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“…extra energy of 329 kJ/mol CH 4 could be generated through the co-combustion of nitrous oxide with methane compared to the combustion of methane with oxygen. As such, extensive efforts have been devoted to developing novel processes for enhanced nitrous oxide production from wastewater (Lin et al, 2018;Myung et al, 2015). As illustrated in Figure 2.8, nitrous oxide can be generated through biological or coupled biological-chemical pathways in wastewater treatment (Zhang et al, 2019).…”

Section: Energy Recovery From Nitrous Oxidementioning

confidence: 99%

Approaches to energy and resource recovery from municipal wastewater

Zhang¹

2020

A-B Processes: Towards Energy Self-Sufficient Municipal Wastewater Treatment

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The activated sludge process is regarded by many as a remarkable engineering development of the 20th Century which has made a great contribution to wastewater treatment. But after more than 100 years of successful application, the conventional activated sludge (CAS) process has recently been receiving an increasing number of critiques due to the high energy consumption and excessive sludge generation associated with it. Increasing efforts have been devoted to energy recovery in the form of biogas produced from waste sludge through anaerobic digestion (AD). However, the recoverable electrical energy generated from the AD of waste sludge can only offset about 50-60% of the total input energy of wastewater treatment plants (WWTPs) with CAS as the core process, while energy efficiency could be increased further to about 75% with upgraded AD equipped with pre-treatment of thickening sludge and a combined heat and power (CHP) process (Cao, 2011). Moreover, process upgrading and retrofitting are urgently needed in more and more WWTPs around the world to meet the tightened effluent discharge standards applied in recent years which inevitably lead to a rising trend of in-plant energy consumption. For example, it has been reported that the effluent discharge standards in China have been gradually migrated to quasi Class IV for surface water bodies with very low discharge limits. Consequently, it appears almost impossible or highly challenging to

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Significant enhancement of nitrous oxide energy yields from wastewater achieved by bioaugmentation with a recombinant strain of Pseudomonas aeruginosa

Cited by 19 publications

References 46 publications

Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis

Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis

Recovery of Nitrous Oxide from Wastewater Treatment: Current Status and Perspectives

Approaches to energy and resource recovery from municipal wastewater

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