Significant enhancement of oleanolic acid accumulation by biotic elicitors in cell suspension cultures of Calendula officinalis L.

Wiktorowska, Ewa; Długosz, Marek; Janiszowska, Wirginia

doi:10.1016/j.enzmictec.2009.09.002

Cited by 119 publications

(68 citation statements)

References 33 publications

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“…Purified OA was methylated with an ether solution of diazomethane (Janiszowska and Kasprzyk 1977). Detailed description of the extraction procedure was published previously by Wiktorowska et al (2010) and Długosz et al (2013).…”

Section: Extraction Of Oleanolic Acidmentioning

confidence: 99%

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

2018

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Triterpenic saponins represented in Calendula officinalis L. by oleanolic acid (OA) glycosides are pentacyclic triterpene compounds with a wide range of biological and medicinal properties. This report demonstrates nitrogen source impact on growth, saponin accumulation, and secretion in hairy root and suspension cultures of marigold. Hairy roots preferred nitrate as a mineral source of nitrogen, but its impact on growth, OA glycosides accumulation, and secretion were line-dependent. The best productivity of OA glycosides was found in CC16 line (74.86 mg flask −1 ) in ½ MS medium modified by 2.5× KNO 3 and ammonium elimination with 2.5 g l −1 peptone. Organic nitrogen source at 27.5-g l −1 impairs the growth rate of hairy roots. Its effect on saponin accumulation and secretion to the surrounding medium depended on line and media composition. Nitrate:ammonium ratio of 4:2 for CC16 resulted in 5.7-fold increment of saponin secretion comparing to the standard medium. Embryo roots, apical bud, and hypocotyls explants were crucial for induction of suspension culture synthesizing saponins; however, effect of mineral form of nitrogen in cultivating medium had to be considered. The highest OA glycosides level (171.97 μg g −1 of dry weight) was recorded in the root derived culture with nitrate as a sole mineral form of nitrogen. Peptone from lactalbumin decidedly inhibited the saponin formation; however, it was essential for culture initiation, proliferation, and organ differentiation.

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Section: Extraction Of Oleanolic Acidmentioning

confidence: 99%

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

2018

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“…Both chitin and chitosan have demonstrated antiviral, antibacterial, and antifungal properties, and have been explored for many agricultural uses [El Hadrami et al 2010]. Chitosan is an exogenous biotic elicitor that is derived from the fungal cell wall [Montesano et al 2003], it has been studied for their effects on phenylpropanoid metabolic enzymes [Chakraborty et al 2009], and secondary metabolite production [Wiktorowska et al 2010].…”

Section: Introductionmentioning

confidence: 99%

Effects of Foliar of the Application Chitosan and Reduced Irrigation on Essential Oil Yield, Total Phenol Content and Antioxidant Activity of Extracts From Green and Purple Basil

Pirbalouti¹,

Malekpoor²,

Salimi³

et al. 2017

Acta Sci. Pol. Hortorum Cultus

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Phenolic compounds are naturally occurring substances in plants. Basil (Ocimum basilicum L.) belongs to the family Lamiaceae, is a good source of phenolic compounds and natural antioxidants. Elicitors can activate specific genes involved in secondary metabolite biosynthesis. Chitosan, as elicitor, is a natural biopolymer modified from chitin, which is the main structural component of squid pens, cell walls of some fungi and shrimp and crab shells. On the other hand, water deficit stress is one of the most abiotic stress, which effects on the levels of secondary metabolites. To evaluate the effect of chitosan and different irrigation regimes on essential oil yield, total phenol content and antioxidant activity of extracts from green and purple basil (Ocimum basilicum L.), an experiment was conducted at Shahrekord, southwestern Iran. Treatments comprised control, 0.0, 0.2, and 0.4 g·L -1 chitosan applied to plants under normal irrigation, slight and mild drought stress conditions. Results indicated that the different levels of chitosan and irrigation had significant effects on the essential oil yield, total phenol content, and the antioxidant activity of the extracts. Foliar-applied chitosan in particular 0.4 g·L -1 increased total phenolic content in the basil as compared to untreated plants. In conclusion, it is suggested that the foliar application of chitosan as an elicitor could be a promising material used to increase biological activity and pro-health functional value of basil plants.

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“…Chitosan and salicylic acid have been reported to enhance the in vitro production of secondary metabolites in various plants [32] , such a stimulatory effect may be due to the stimulation of biosynthetic enzymes [33] . Chitosan is the main structural component of the cell wall of plant pathogen fungi, which mimics its effects and activates the biosynthesis of defense-related secondary metabolites in plants [8,11,34] .Also, salicylic acid has an important role in the defense against attacks by microbes and herbivores, and against abiotic stresses, hence, it is very often used in plant in vitro cultures as an elicitor [32,35] . It was observed that chitosan was better for the enhancement of the production of BITC than salicylic acid.…”

Section: Elicitationmentioning

confidence: 99%

“…They are highly effective for proper mixing of cell suspensions and the break-up of air bubbles to enhance oxygenation and prevent large cell aggregates formation [7] . Elicitation is one the most effective strategies for improving the in vitro production of secondary metabolites [8] .Elicitors can stimulate plant defense, leading to the enhanced synthesis and accumulation of secondary metabolites [2] .According to their origin, elicitors are divided into abiotic and biotic elicitors. Abiotic elicitors are substances of non-biological origin, being inorganic compounds; such as salts or physical factors [9] .Biotic elicitors include substances of pathogenic origin (exogenous elicitors) and compounds produced by plants after the action of the pathogen (endogenous elicitors) [3,10] .Chitosan and salicylic acid are biotic elicitors.…”

Section: Introductionmentioning

confidence: 99%

Establishment of suspension cultures of Salvadora persica L. for benzyl isothiocyanate production

Hegazi¹

2017

IOSRJPBS

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Significant enhancement of oleanolic acid accumulation by biotic elicitors in cell suspension cultures of Calendula officinalis L.

Cited by 119 publications

References 33 publications

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L.

Effects of Foliar of the Application Chitosan and Reduced Irrigation on Essential Oil Yield, Total Phenol Content and Antioxidant Activity of Extracts From Green and Purple Basil

Establishment of suspension cultures of Salvadora persica L. for benzyl isothiocyanate production

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