Significant roles in RNA-binding for the amino-terminal domains of Drosophila Pumilio and Nanos

Wharton, Tammy H.; Marhabaie, Mohammad; Wharton, Robin P.

doi:10.1101/2023.10.24.563753

Cited by 1 publication

(2 citation statements)

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“…3C). All of these observations are consistent with work described elsewhere showing that Nos+Pum bind efficiently to NREs with either U or A at position +4 (T. H. Wharton et al, 2023).…”

Section: Upf1-nos Acts Primarily In Conjunction With Pumsupporting

confidence: 92%

“…The correlation that we do observe (Fig 3C) is consistent with the idea that higher site density promotes cooperative interactions among nearby bound complexes. This model is supported by experiments described elsewhere showing that the N-terminal domain of Pum interacts with itself (Wharton et al, 2023). Finally, the contribution of other RNA-bound regulators or RNA structure is too complex to currently be taken into account when considering the entire transcriptome, as has been pointed out previously (26).…”

Section: Discussionsupporting

confidence: 54%

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Widespread regulation of the maternal transcriptome by Nanos in Drosophila

Marhabaie,

Wharton,

Kim

et al. 2023

Preprint

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The translational repressor Nanos (Nos) regulates a single target, maternalhunchback(hb)mRNA, to govern abdominal segmentation in the early Drosophila embryo. Nos is recruited specifically to sites in the 3’-UTR ofhbmRNA in collaboration with the sequence-specific RNA-binding protein Pumilio (Pum); on its own, Nos has no binding specificity. Nos is expressed at other stages of development, but very few mRNA targets that might mediate its action at these stages have been described. Nor has it been clear whether Nos is targeted to other mRNAs in concert with Pum or via other mechanisms. In this report, we identify mRNAs targeted by Nos via two approaches. In the first method, we identify mRNAs depleted upon expression of a chimera bearing Nos fused to the nonsense mediated decay (NMD) factor Upf1. We find that, in addition tohb, Upf1-Nos depletes ∼2600 mRNAs from the maternal transcriptome in early embryos. Virtually all of these appear to be targeted in a canonical,hb-like manner in concert with Pum. In a second, more conventional approach, we identify mRNAs that are stabilized during the maternal zygotic transition (MZT) in embryos fromnos-females. Most (86%) of the 1185 mRNAs regulated by Nos are also targeted by Upf1-Nos, validating use of the chimera. Approximately 60% of mRNAs targeted by Upf1-Nos are not stabilized in the absence of Nos. However, Upf1-Nos mRNA targets are hypo-adenylated and inefficiently translated at the ovary-embryo transition, whether or not they suffer Nos-dependent degradation in the embryo. We suggest that the late ovarian burst of Nos represses a large fraction of the maternal transcriptome, priming it for later degradation by other factors during the MZT in the embryo.

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“…3C). All of these observations are consistent with work described elsewhere showing that Nos+Pum bind efficiently to NREs with either U or A at position +4 (T. H. Wharton et al, 2023).…”

Section: Upf1-nos Acts Primarily In Conjunction With Pumsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 54%

Widespread regulation of the maternal transcriptome by Nanos in Drosophila

Marhabaie,

Wharton,

Kim

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Significant roles in RNA-binding for the amino-terminal domains of Drosophila Pumilio and Nanos

Cited by 1 publication

References 44 publications

Widespread regulation of the maternal transcriptome by Nanos in Drosophila

Widespread regulation of the maternal transcriptome by Nanos in Drosophila

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