Significantly enhanced osteoblast response to nano-grained pure tantalum

Huo, Wangtu; Zhao, Lei; Yu, Sen; Yu, Zhentao; Zhang, P. X.; Zhang, Y. S.

doi:10.1038/srep40868

Cited by 56 publications

(38 citation statements)

References 52 publications

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“…33 In addition, a nanocrystalline Ta surface possessed higher surface hydrophilicity and enhanced corrosion resistance than those of conventional coarse-grained Ta. 34 Therefore, it was interesting to study how uncombined Ta nanoparticles behave. In the present study, MC3T3-E1 osteoblasts were cultured with 1, 10, and 20 μg/mL uncombined Ta-NPs, revealing that the cell viability increased compared with that of the untreated group, indicating that Ta-NPs can also promote osteoblast proliferation.…”

Section: Discussionmentioning

confidence: 99%

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Kang¹,

Wei²,

Song³

et al. 2017

IJN

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Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect.

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Section: Discussionmentioning

confidence: 99%

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Kang¹,

Wei²,

Song³

et al. 2017

IJN

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“…In recent years, tantalum has received a considerable amount of attention regarding its application in the biomedical sector. The metal was known to exhibit good biocompatibility since the 1940s [ 1 , 2 , 3 ]. However, its high cost and density explain why it has not been researched as vigorously as titanium and its alloys.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Impedance and Polarization Corrosion Studies of Tantalum Surface Modified by DC Plasma Electrolytic Oxidation

Sowa

Simka

2018

Materials

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Tantalum has recently become an actively researched biomaterial for the bone reconstruction applications because of its excellent corrosion resistance and successful clinical records. However, a bare Ta surface is not capable of directly bonding to the bone upon implantation and requires some method of bioactivation. In this study, this was realized by direct current (DC) plasma electrolytic oxidation (PEO). Susceptibility to corrosion is a major factor determining the service-life of an implant. Therefore, herein, the corrosion resistance of the PEO coatings on Ta was investigated in Ringer’s solution. The coatings were formed by galvanostatic anodization up to 200, 300 and 400 V, after which the treatment was conducted potentiostatically until the total process time amounted to 5 min. Three solutions containing Ca(H2PO2)2, Ca(HCOO)2 and Mg(CH3COO)2 were used in the treatment. For the corrosion characterization, electrochemical impedance spectroscopy and potentiodynamic polarization techniques were chosen. The coatings showed the best corrosion resistance at voltages low enough so that the intensive sparking was absent, which resulted in the formation of thin films. The impedance data were fitted to the equivalent electrical circuits with two time constants, namely R(Q[R(QR)]) and R(Q[R(Q[RW])]). The inclusion of W in the circuit helped to fit the low-frequency part of the samples PEO-ed at 400 V, hinting at the important role of diffusion in the corrosion resistance of the PEO coatings described in the research.

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“…Results have shown that cellular responses often depend on the mechanical properties, pattern structures, and surface chemistry of the microenvironment surrounding the cells [1,3,4,5,7,8,10,11,12,13,14,15,16,17,18,19,20]; however, work done thus far has been conducted with structures composed of monolithic materials having uniform surface chemical composition. Studies of cell behavior on nanocomposite surfaces are limited [1,3,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

et al. 2018

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The primary goal of this work was to investigate the resulting morphology of a mammalian cell deposited on three-dimensional nanocomposites constructed of tantalum and silicon oxide. Vero cells were used as a model. The nanocomposite materials contained comb structures with equal-width trenches and lines. High-resolution scanning electron microscopy and fluorescence microscopy were used to image the alignment and elongation of cells. Cells were sensitive to the trench widths, and their observed behavior could be separated into three different regimes corresponding to different spreading mechanism. Cells on fine structures (trench widths of 0.21 to 0.5 μm) formed bridges across trench openings. On larger trenches (from 1 to 10 μm), cells formed a conformal layer matching the surface topographical features. When the trenches were larger than 10 μm, the majority of cells spread like those on blanket tantalum films; however, a significant proportion adhered to the trench sidewalls or bottom corner junctions. Pseudopodia extending from the bulk of the cell were readily observed in this work and a minimum effective diameter of ~50 nm was determined for stable adhesion to a tantalum surface. This sized structure is consistent with the ability of pseudopodia to accommodate ~4–6 integrin molecules.

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Significantly enhanced osteoblast response to nano-grained pure tantalum

Cited by 56 publications

References 52 publications

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Electrochemical Impedance and Polarization Corrosion Studies of Tantalum Surface Modified by DC Plasma Electrolytic Oxidation

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

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