Significantly improved detection performances of immunoassay for ractopamine in urine based on highly urea-tolerant rabbit monoclonal antibody

Response to Comments on “Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”

“…To achieve efficient and precise screening of hapten-specific antibody-secreting cells, our work in 2022 proposed an indirect competitive screening method in nanowells, named CSMN, that conducts a completive ELISA in a nanowell to specifically select the hapten-specific antibody-secreting cells, which is an efficient platform for hapten-specific antibody production (Figure ). We have successfully produced rabbit monoclonal antibodies with high affinity and specificity based on the CMSN …”

Nafion/Silver Nanoparticles as an Electrochemically Sensitive Interface for the Detection of Ractopamine in Pork Liver

“…10−17 ELISA offers a rapid and portable method for detecting Rac; however, its major drawback is its requirements of complicated time-consuming sample pretreatment and preparation because of the complex matrix of animal tissues. 14 Among instrumental techniques, HPLC enables multicomponent and highly sensitive analysis; however, the HPLC instrument is costly and nonportable, which prevents its widespread application. 18 Moreover, welltrained personnel are required to operate this instrument.…”

“…Currently, analytical methods for detecting Rac in pork are limited to techniques requiring highly sophisticated sample preparation or costly instrumentation, such as enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC–MS), and liquid chromatography–tandem mass spectrometry (LC–MS/MS). − ELISA offers a rapid and portable method for detecting Rac; however, its major drawback is its requirements of complicated time-consuming sample pretreatment and preparation because of the complex matrix of animal tissues . Among instrumental techniques, HPLC enables multicomponent and highly sensitive analysis; however, the HPLC instrument is costly and nonportable, which prevents its widespread application .…”