Sikyungbanha‐Tang Suppressing Acute Lung Injury in Mice Is Related to the Activation of Nrf2 and TNFAIP3

Kim, Kyun Ha; Ahn, Seonju; Won, Ran; Lee, Jung Ju; Kim, Tae Ho; Kim, Jong-In; Choi, Jun‐Yong; Joo, Myungsoo

doi:10.1155/2020/8125758

Cited by 5 publications

(5 citation statements)

References 51 publications

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“…5A, B). We found that chemokines (GPR-35, CXCL15, CCL5, and CCR2) involved in the macrophages and neutrophils recruitment [19,[23][24][25] were significantly elevated, while the negative regulator of macrophages activation KLF2 was downregulated in IL-10 B-KO lungs (Fig. 5C).…”

Section: Il-10 In B Cells Promotes Ali Recovery Possibly Via the Cgmp...mentioning

confidence: 86%

B cell-derived IL-10 promotes the resolution of lipopolysaccharide-induced acute lung injury

et al. 2023

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Inflammation resolution is critical for acute lung injury (ALI) recovery. Interleukin (IL)-10 is a potent anti-inflammatory factor. However, its role in ALI resolution remains unclear. We investigated the effects of IL-10 during the ALI resolution process in a murine lipopolysaccharide (LPS)-induced ALI model. Blockade of IL-10 signaling aggravates LPS-induced lung injury, as manifested by elevated pro-inflammatory factors production and increased neutrophils recruitment to the lung. Thereafter, we used IL-10 GFP reporter mice to discern the source cell of IL-10 during ALI. We found that IL-10 is predominantly generated by B cells during the ALI recovery process. Furthermore, we used IL-10-specific loss in B-cell mice to elucidate the effect of B-cell-derived IL-10 on the ALI resolution process. IL-10-specific loss in B cells leads to increased pro-inflammatory cytokine expression, persistent leukocyte infiltration, and prolonged alveolar barrier damage. Mechanistically, B cell-derived IL-10 inhibits the activation and recruitment of macrophages and downregulates the production of chemokine KC that recruits neutrophils to the lung. Moreover, we found that IL-10 deletion in B cells leads to alterations in the cGMP–PKG signaling pathway. In addition, an exogenous supply of IL-10 promotes recovery from LPS-induced ALI, and IL-10-secreting B cells are present in sepsis-related ARDS. This study highlights that B cell-derived IL-10 is critical for the resolution of LPS-induced ALI and may serve as a potential therapeutic target.

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Section: Il-10 In B Cells Promotes Ali Recovery Possibly Via the Cgmp...mentioning

confidence: 86%

B cell-derived IL-10 promotes the resolution of lipopolysaccharide-induced acute lung injury

et al. 2023

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“…Tumor necrosis factor alpha-induced protein 3 (TNFAIP3) is an important deubiquitinating enzyme that has an impact on tumorigenesis, immune responses, and inflammation [ 30 , 31 ]. The function of TNFAIP3 mainly relies on its ubiquitin degradation to regulate intracellular protein expression [ 32 ]. Recent studies suggested that TNFAIP3 may have a vital role in the invasion and proliferation of lung cancer and gastric cancer [ 31 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals the differential inflammatory effects between propofol and sevoflurane during lung cancer resection: a randomized pilot study

Wang

Cai

et al. 2023

World J Surg Onc

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Background Propofol and sevoflurane are two commonly used perioperative anesthetics. Some studies have found that these anesthetic drugs affect tumorigenesis. Previous studies have mostly focused on in vitro experiments, and the specimens collected were mainly peripheral body fluids, lacking direct evidence of the impact of anesthetic drugs on human tissues. This study aimed to elucidate the effects of propofol and sevoflurane on lung cancer using next-generation sequencing through an in vivo experiment. Methods Patients were randomly assigned to a group receiving either propofol or sevoflurane during surgery. Then, the patients’ tumor and paired normal samples were collected and sequenced by next-generation sequencing. Differentially expressed genes (DEG) were analyzed by two statistical models, followed by cluster analysis, PCA, Gene Ontology, and KEGG pathway analysis. Candidate genes were confirmed by qRT–PCR. Results The demographic data of the two study groups were not statistically significant. Through single-factor model analysis, 810 DEG in the propofol group and 508 DEG in the sevoflurane group were obtained. To better reflect the differential effects between propofol and sevoflurane while reducing the false-positive DEG, we used multifactor model analysis, which resulted in 124 DEG. In PCA and cluster analysis, four groups (propofol cancer group, propofol normal group, sevoflurane cancer group, sevoflurane normal group) were separated adequately, indicating the accuracy of the analysis. We chose seven significant pathways (cellular response to interleukin-1, chemokine-mediated signaling pathway, chemokine signaling pathway, cytokine–cytokine receptor interaction, inflammatory response, immune response, and TNF signaling pathway) for downstream analysis. Based on the pathway analysis, three candidate genes (CXCR1, CXCL8, and TNFAIP3) were chosen, and their qRT–PCR results were consistent with the sequencing results. Conclusions Through RNA-seq analysis, the effects of propofol and sevoflurane during lung cancer resection were different, mainly in inflammatory-related pathways, which might be possibly by targeting CXCL8. Trial registration Trial registry number was ChiCTR1900026213.

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“…Tumor necrosis factor alpha-induced protein 3 (TNFAIP3) is an important deubiquitinating enzyme that has an impact on the tumorigenesis, immune responses and in ammation (Du et al 2019;Liao et al 2020). The function of TNFAIP3 mainly relies on its ubiquitin degradation for intracellular protein expression (Kim et al 2020). Recent studies suggested that TNFAIP3 may have vital role in the invasion and proliferation of lung cancer and gastric cancer (Liao et al 2020;Wisnieski et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Differential Inflammatory Effects Between Propofol and Sevoflurane During Lung Cancer Resection: a Randomized Pilot Study

Zhang¹,

Wang²,

Cai³

et al. 2021

Preprint

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Background: Propofol and sevoflurane are two commonly used anesthetics perioperatively. It showed that anesthetic drugs may have an effect on tumorigenesis. Previous researches mostly focused on in vitro experiments, the specimens collected were mostly peripheral body fluid, lacking direct evidence of the impact of anesthetic drugs on human tissues. This study aims at elucidating the effects of propofol and sevoflurane on lung cancer using next-generation sequencing through an in vivo experiment. Methods: Patients were randomly assigned to a group either receiving propofol or sevoflurane during surgery. Then patients’ tumor and paired normal samples were collected and sequenced by next-generation sequencing. Differentially expressed genes (DEG) were analyzed by two statistical models, then cluster analysis, PCA, GO ontology and KEGG pathways analysis were done. Candidate genes were confirmed by qRT-PCR. Results: Two study groups demographic data were not statistically significant. Through single factor model analysis, 810 DEG in propofol group and 508 DEG in sevoflurane group were obtained. In order to better reflect the differential effects of propofol and sevoflurane, in the meanwhile reduce false positives of DEG, we used multi-factor model analysis, which resulted in 124 DEG. In PCA and cluster analysis, four groups (propofol cancer group, propofol normal group, sevoflurane cancer group, sevoflurane normal group) were separated adequately, indicating the accuracy of DEG. We chose seven significant pathways (Cellular response to interleukin-1, Chemokine-mediated signaling pathway, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Inflammatory response, Immune response, TNF signaling pathway) for downstream analysis. From qRT-PCR results, three genes (CXCR1, CXCL8 and TNFAIP3) were confirmed. Conclusions: Through RNA-seq analysis, the effects of propofol and sevoflurane during lung cancer resection were different, and mainly differ in inflammatory related pathways which might be possibly by targeting CXCL8.Trial registration: Trial registry number was ChiCTR1900026213 (http://www.chictr.org.cn/showproj.aspx?proj=43733).

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Sikyungbanha‐Tang Suppressing Acute Lung Injury in Mice Is Related to the Activation of Nrf2 and TNFAIP3

Cited by 5 publications

References 51 publications

B cell-derived IL-10 promotes the resolution of lipopolysaccharide-induced acute lung injury

B cell-derived IL-10 promotes the resolution of lipopolysaccharide-induced acute lung injury

Transcriptome analysis reveals the differential inflammatory effects between propofol and sevoflurane during lung cancer resection: a randomized pilot study

Transcriptome Analysis Reveals the Differential Inflammatory Effects Between Propofol and Sevoflurane During Lung Cancer Resection: a Randomized Pilot Study

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