Silaffin-3-derived pentalysine cluster as a new fusion tag for one-step immobilization and purification of recombinant Bacillus subtilis catalase on bare silica particles

Cascade chiral amine synthesis catalyzed by site-specifically co-immobilized alcohol and amine dehydrogenases

“…, silica, carbon materials, and metals) with high site-specificity and positional precision. 20,29–33 Moreover, SBPs exhibit high binding affinity to a given solid material based on the physicochemical effects at the interface that comprise van der Waals, hydrogen bonding, and Coulombic forces, as well as surface diffusion and structural changes. 20,24,26,34,35 Hence, SBPs have been extensively developed and applied in enzyme immobilization.…”

“…20,24,26,34,35 Hence, SBPs have been extensively developed and applied in enzyme immobilization. 30,31,36,37 Among common solid carriers, silica displays superiority for enzyme immobilization because of its advantages of tunable pore size, high specific surface area, stable chemical and physical property, and good biocompatibility. 38 Therefore, silica binding peptide (SiBP) is highly potential and attractive in enzymes immobilization.…”

“…38,39 However, most of the immobilized SiBP fusion enzymes have been reported to display poor catalytic efficiency, probably due to the use of micron-sized solid particles with small specific surface areas representing low enzyme loading. 30,31 Moreover, the literature studies with SiBP fusion enzymes have focused on single enzyme immobilizations. 30,31,36,37…”

“…30,31 Moreover, the literature studies with SiBP fusion enzymes have focused on single enzyme immobilizations. 30,31,36,37…”

Use of Natural Waste Carrier in Enzyme Immobilization: Catalase Immobilization Onto Eggshell Membrane

“…In addition, it is also used in the dairy industry to decompose the hydrogen peroxide released during the cold pasteurization process of milk [16]. It has been reported that catalase enzyme has been immobilized on particles, cryogels, gels, polymeric nanostructures, nanoparticles, nanocomposites and nanofibers in the literature [17][18][19][20][21]. In this study, catalase was immobilized on ESM using adsorption and cross-linking methods.…”