Sildenafil citrate induces migration of mouse aortic endothelial cells and proteinase secretion

Kim, Young Il; Oh, In Suk; Park, Seung Moon; Kim, Hwan Gyu

doi:10.1007/bf02932306

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…The human prostate adenocarcinoma PC-3 cell line was acquired from the Korean Cell Line Bank (Seoul, Korea). The cells were maintained at 37 o C under 5% CO2 in RPMI-1640 medium that was supplemented with 10% (v/v) heat-inactivated FBS [11,14]. For siRNA transfection, the PC-3 cells (5x10 5 ) were washed in sterile PBS and then replenished in supplemented RPMI-1640 medium for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Cobalt Chloride-Induced Down-Regulation of Puromycin-Sensitive Aminopeptidase Involved in Apoptosis of PC-3 Cells

Lee¹,

Kim

2010

Journal of Life Science

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Hypoxia is an indicative of pro-apoptotic and anti-apoptotic biphasic effects, which appear to be dependent upon the cell type and the condition of the cells. The hypoxia-mimetic agent, cobalt chloride (CoCl2), has been shown to induce apoptosis in a variety of cell types, but the mechanism by which this occurs has yet to be thoroughly elucidated. Puromycin-sensitive aminopeptidase (PSA) gene was decreasingly expressed in response to CoCl2. In this report, puromycin pretreatment applied to PC-3 cells resulted in apoptosis. To determine whether PSA is involved in apoptosis, we examined the apoptotic properties of the PC-3 cells after siRNA knockdown of PSA. PSA siRNA-induced PSA silencing revealed that endogenous PSA may be involved in apoptosis of the PC-3 cells. These results indicated that PSA may perform a vital function in cell survival of the PC-3 cells.

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Section: Methodsmentioning

confidence: 99%