Silencing of a <i>LIM</i> gene in cotton exhibits enhanced resistance against <i>Apolygus lucorum</i>

Liang, Shi-Dong; Luo, Jing; Alariqi, Muna; Xu, Zhongping; Wang, Aoli; Zafar, Muhammad Naeem; Ren, Jun; Wang, Fuqiu; Liu, Xuefei; Xin, Yanfeng; Xu, Haonan; Guo, Weifeng; Wang, Yanqin; Ma, Wanjun; Chen, Lizhen; Lindsey, Keith; Zhang, Xianlong; Jin, Shuangxia

doi:10.1002/jcp.30281

Cited by 12 publications

(8 citation statements)

References 57 publications

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“…All the tissue culture and callus induction and proliferation experiments of upland cotton ( Gossypium hirsutum L.) Texas Marker-1 (TM-1) and Jin668 [ 6 ] were performed at Huazhong Agricultural University (Wuhan, Hubei, China) following our previous report [ 9 , 76 ]. The hypocotyls of etiolated seedlings were cut into 5–7 mm sections and used as explants for callus induction on MSB (2,4-D) medium.…”

Section: Methodsmentioning

confidence: 99%

Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem

Zhu,

Xu,

Wang

et al. 2023

Genome Biol

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Background Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technologies enable us to explore the mechanism of plant regeneration at single-cell resolution. Results We generate a high-resolution single-cell transcriptomic landscape of hypocotyl tissue from the highly regenerable cotton genotype Jin668 and the recalcitrant TM-1. We identify nine putative cell clusters and 23 cluster-specific marker genes for both cultivars. We find that the primary vascular cell is the major cell type that undergoes cell fate transition in response to external stimulation. Further developmental trajectory and gene regulatory network analysis of these cell clusters reveals that a total of 41 hormone response-related genes, including LAX2, LAX1, and LOX3, exhibit different expression patterns in the primary xylem and cambium region of Jin668 and TM-1. We also identify novel genes, including CSEF, PIS1, AFB2, ATHB2, PLC2, and PLT3, that are involved in regeneration. We demonstrate that LAX2, LAX1 and LOX3 play important roles in callus proliferation and plant regeneration by CRISPR/Cas9 editing and overexpression assay. Conclusions This study provides novel insights on the role of the regulatory network in cell fate transition and reprogramming during plant regeneration driven by somatic embryogenesis.

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Section: Methodsmentioning

confidence: 99%

Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem

Zhu,

Xu,

Wang

et al. 2023

Genome Biol

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“…The full-length CDS and RNAi fragments of GhMPK31 were separately amplified and inserted into the pK2GW7 and pHellsgate 4 vectors using the Gateway cloning technology (Karimi et al 2002 ; Liang et al 2021 ; Luo et al 2017 ; Tian et al 2015 ; Wesley et al 2001 ). The recombinant vectors for overexpression and RNAi were transformed into the cotton (cv.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive analysis of MAPK gene family in upland cotton (Gossypium hirsutum) and functional characterization of GhMPK31 in regulating defense response to insect infestation

Wang,

Liang,

Wang

et al. 2024

Plant Cell Rep

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Key message The transcriptomic, phenotypic and metabolomic analysis of transgenic plants overexpressing GhMPK31 in upland cotton revealed the regulation of H2O2 burst and the synthesis of defensive metabolites by GhMPK31. Abstract Mitogen-activated protein kinases (MAPKs) are a crucial class of protein kinases, which play an essential role in various biological processes in plants. Upland cotton (G. hirsutum) is the most widely cultivated cotton species with high economic value. To gain a better understanding of the role of the MAPK gene family, we conducted a comprehensive analysis of the MAPK gene family in cotton. In this study, a total of 55 GhMPK genes were identified from the whole genome of G. hirsutum. Through an investigation of the expression patterns under diverse stress conditions, we discovered that the majority of GhMPK family members demonstrated robust responses to abiotic stress, pathogen stress and pest stress. Furthermore, the overexpression of GhMPK31 in cotton leaves led to a hypersensitive response (HR)-like cell death phenotype and impaired the defense capability of cotton against herbivorous insects. Transcriptome and metabolomics data analysis showed that overexpression of GhMPK31 enhanced the expression of H2O2-related genes and reduced the accumulation of defensive related metabolites. The direct evidence of GhMPK31 interacting with GhRBOHB (H2O2-generating protein) were found by Y2H, BiFC, and LCI. Therefore, we propose that the increase of H2O2 content caused by overexpression of GhMPK31 resulted in HR-like cell death in cotton leaves while reducing the accumulation of defensive metabolites, ultimately leading to a decrease in the defense ability of cotton against herbivorous insects. This study provides valuable insights into the function of MAPK genes in plant resistance to herbivorous insects.

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“…A series of genes related to the biosynthesis of phenylpropanoid, α‐linolenic acid (LA), flavone, and the pathway of plant hormone signal transduction may be involved in the regulation of Bt cotton to the defense response of A. lucorum (Chen et al, 2022 ). Silencing of the LIM gene in cotton results in strong resistance to A. lucorum (Liang et al, 2021 ). These studies provide insight into the resistance levels of various host plants against A. lucorum , as well as the selection and adaptation mechanisms of A. lucorum to different host plants (Wang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding

Yao,

Gao,

Sun

et al. 2024

Plant Direct

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To investigate the molecular mechanism of the defense response of “Cabernet Sauvignon” grapes to feeding by Apolygus lucorum, high‐throughput sequencing technology was used to analyze the transcriptome of grape leaves under three different treatments: feeding by A. lucorum, puncture injury, and an untreated control. The research findings indicated that the differentially expressed genes were primarily enriched in three aspects: cellular composition, molecular function, and biological process. These genes were found to be involved in 42 metabolic pathways, particularly in plant hormone signaling metabolism, plant‐pathogen interaction, MAPK signaling pathway, and other metabolic pathways associated with plant‐induced insect resistance. Feeding by A. lucorum stimulated and upregulated a significant number of genes related to jasmonic acid and calcium ion pathways, suggesting their crucial role in the defense molecular mechanism of “Cabernet Sauvignon” grapes. The consistency between the gene expression and transcriptome sequencing results further supports these findings. This study provides a reference for the further exploration of the defense response in “Cabernet Sauvignon” grapes by elucidating the expression of relevant genes during feeding by A. lucorum.

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Silencing of a LIM gene in cotton exhibits enhanced resistance against Apolygus lucorum

Cited by 12 publications

References 57 publications

Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem

Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem

Comprehensive analysis of MAPK gene family in upland cotton (Gossypium hirsutum) and functional characterization of GhMPK31 in regulating defense response to insect infestation

Transcriptomic analysis of the defense response in “Cabernet Sauvignon” grape leaf induced by Apolygus lucorum feeding

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