Silencing of Aphid Genes by dsRNA Feeding from Plants

Coleman, Alexander D.; Mugford, Sam T.; Hogenhout, Saskia A.

doi:10.1007/978-3-319-24049-7_10

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…4 and 5). Previous studies showed that target genes in different insects including Myzus persicae, S. avenae, and B. tabaci MED could be silenced by the delivery of dsRNAs via transgenic plants (Abdellatef et al, 2015;Coleman et al, 2016;Xia et al, 2021). The CAT gene is not a direct lethal gene, but silencing of the CAT gene may be useful for the control of B. tabaci MED in regions with high temperatures.…”

Section: Discussionmentioning

confidence: 99%

Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species

et al. 2022

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Thermal stress usually leads to excessive production of reactive oxygen species (ROS) in all aerobic organisms. Catalases (CAT) are the key antioxidant enzymes, which act as the first line of defense against ROS in the antioxidant pathway. The highly invasive and widely distributed whitefly Bemisia tabaci MED damages plants by feeding as well as by transmitting many plant viruses. Previous studies have shown that strong adaptability to high temperature helps explain the spread of MED around the world. However, the mechanism underlying high temperature adaptation of this pest is not well understood. In this study, 6 CAT genes were identified from the MED genome and transcriptome dataset, among which BtCAT1, BtCAT2, and BtCAT3 were found to be highly expressed in adults. The expression of BtCAT1, BtCAT2, or BtCAT3 increased with induction temperature and induction time. The MED was exposed with mean high temperature (30 °C or 35 °C) and a short‐term extremely high temperature (39 °C or 41 °C) after the silencing of BtCAT1, BtCAT2, or BtCAT3 to significantly increased ROS levels by at least 0.5 times and significantly decreased survival rate and fecundity of MED adults. The ROS level in the treated specimens gradually returned to a normal level after 24 h at 25 °C, but the survival rate still declined significantly. Taken together, our results demonstrate that CAT could help B. tabaci adapt to long‐term mean high temperatures and short‐term extremely high temperatures by eliminating excessive ROS.

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Section: Discussionmentioning

confidence: 99%

Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species

et al. 2022

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“…Для подходов, основанных на РНКи, обусловленной трансгенными растениями, широко используется зеленая персиковая тля M. persicae. Она способна питаться такими модельными растениями как Nicotiana benthamiana Domin и Arabidopsis thaliana (L.) Heynh., тогда как у гороховой тли A. pisum набор кормовых растений намного уже, поскольку она питается только бобовыми (Coleman et al, 2016). РНКи была использована для подавления благоприятствующих тлям генов растений, участвующих в ослаблении защитной реакции растений или облегчающих насекомым доступ к пище и улучшающих ее питательную ценность (Ahman et al, 2019) (Jain et al, 2021).…”

Section: тлиunclassified

“…Помимо трансгенных растений, постоянно экспрессирующих необходимую дцРНК, для экспериментальных целей можно использовать и временные схемы. Например, дцРНК, соответствующая генам тлей, может продуцироваться временно в листьях N. benthamiana после инфильтрации бактерией Agrobacterium tumeficiens, несущей ДНК, кодирующую дцРНК (Coleman et al, 2016). Препараты дцРНК также можно непосредственно наносить на покровы насекомого.…”

Section: способы доставки дцрнк в организмы тлей и других полужесткок...unclassified

Untitled

2023

1727-1320

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The predatory bug Podisus maculiventris is a polyphagous entomophagous insect, considered as a promising agent of biological protection of potato against the Colorado potato beetle in Western Siberia. In order to reduce the economic costs of its transportation and mass production, it is advisable to improve and adapt the technology of its breeding and application to the conditions of the region. Under laboratory conditions, the effectiveness of different prey species (larvae of the greater wax moth, diamondback moth, yellow mealworm beetle and vetch aphid) to maintain high indicators of the stock culture of the predatory bug was revealed. With a mixed diet, a high survival rate of entomophagous larvae was observed, the number of adults of the next generation averaged 78 % of the initial number, the weight of females and males increased to 75 and 56 mg, respectively, and fertility reached 540 eggs per female. In laboratory conditions, the voracity of the predator varied from 1.8 to 8.4 individuals of the Colorado potato beetle, depending on the stage of development of the entomophagous agent and the age of the pest larvae. As a result of three-year field trials (2018-2020) on potatoes of the Yuna variety, the biological effectiveness of the predator against the Colorado potato beetle ranged from 66 to 85 %, and with the combined use of the predatory bug and the biological preparation Bitoksibatsillin increased to 98.5 %. In 2020, the efficiency of the entomophagous agent released at the predator-prey ratio of 1: 5.0-6.5 ranged between 63 and 75 % on different varieties of potato.

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RNA interference in the study of gene functions in aphids and other hemipterans and the fight against their harmful representatives

Konarev

2023

1727-1320

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RNA interference (RNAi) is an important mechanism that protects cells of eukaryotic organisms from foreign genetic information and regulates physiological processes. Discovery of RNAi and development of the methods for targeted suppression of the expression of individual genes have opened up wide opportunities for elucidating gene functions and solving many problems in biology, medicine, and plant protection. This approach can contribute to the development of new effective, highly specific, and environmentally safe ways to control harmful insects. Several examples of the practical use of RNAi for combating chewing pests have been already reported. Due to their biology, phloem-feeding, as well as the ways of delivering genetic constructs into the body, RNAi in insects with piercing-sucking mouthparts – aphids, bugs, and other hemipterans – deserves separate consideration. This review discusses the criteria for selecting target genes and the issues of their functional activity solved by RNAi, methods for delivering double-stranded and small RNAs to organisms of various Hemiptera, the non-target effects of RNAi and the ways to prevent them, as well as possible practical outcomes of using this approach to control hemipteran pests of agricultural plants and vectors of dangerous human and animal diseases.

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Silencing of Aphid Genes by dsRNA Feeding from Plants

Cited by 7 publications

References 33 publications

Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species

Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species

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RNA interference in the study of gene functions in aphids and other hemipterans and the fight against their harmful representatives

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