Silencing of dre4 Contributes to Mortality of Phyllotreta striolata

Chen, Dongping; Yan, Ru; Xu, Zhanyi; Qian, Jie; Yu, Yinfang; Zhu, Shunshun; Wu, Huiming; Zhu, Guonian; Chen, Mengli

doi:10.3390/insects13111072

Cited by 6 publications

(2 citation statements)

References 52 publications

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“…Third, more research is needed into incorporating natural enemies, including of EPNs and EPFs, into IPM programs and the role of habitat management in conservation biological control programs at local and landscape scales. Fourth, emerging technologies such as RNA interference, based on lethal genes found in SFB (39,58) and CSFB (37), should be explored to develop more specific biopesticides. Fifth, effective control of flea beetles requires the establishment of a comprehensive prediction and forecasting system.…”

Section: Discussionmentioning

confidence: 99%

Biology, Ecology, and Management of Flea Beetles in Brassica Crops

Li,

Costamagna,

Beran

et al. 2024

Annu. Rev. Entomol.

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Brassica vegetable and oilseed crops are attacked by several different flea beetle species (Chrysomelidae: Alticini). Over the past decades, most research has focused on two Phyllotreta species, Phyllotreta striolata and Phyllotreta cruciferae, which are major pests of oilseed rape in North America. More recently, and especially after the ban of neonicotinoids in the European Union, the cabbage stem flea beetle, Psylliodes chrysocephala, has become greatly important and is now considered to be the major pest of winter oilseed rape in Europe. The major challenges to flea beetle control are the prediction of population dynamics in the field, differential susceptibility to insecticides, and the lack of resistant plant cultivars and other economically viable alternative management strategies. At the same time, many fundamental aspects of flea beetle biology and ecology, which may be relevant for the development of sustainable control strategies, are not well understood. This review focuses on the interactions between flea beetles and plants and summarizes the literature on current management strategies with an emphasis on the potential for biological control in flea beetle management.

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Section: Discussionmentioning

confidence: 99%

Biology, Ecology, and Management of Flea Beetles in Brassica Crops

Li,

Costamagna,

Beran

et al. 2024

Annu. Rev. Entomol.

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“…Chen et. al silenced the dre4 gene using the technique of RNA interference and found that the silencing of dre4 contributed to the high mortality of CFB [ 21 ]. CFB adults have active, alert features and high tolerance to pesticides and live on the ground to feed on leaves, while the larvae hide in the soil and infest the seeds and roots.…”

Section: Discussionmentioning

confidence: 99%

Screening of Fungal Strains and Formulations of Metarhizium anisopliae to Control Phyllotreta striolata in Chinese Flowering Cabbage

Chen

Wei

et al. 2023

Insects

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(1) Background: The cabbage flea beetle (CFB; Phyllotreta striolata) seriously damages the production of Chinese flowering cabbage (CFC; Brassica campestris L. ssp. chinensis var. utilis), which is a key leafy vegetable in South China. A large number of chemical insecticides have been sprayed to control this pest; as a result, residues and resistances are becoming an issue. It is necessary to develop biocontrol technologies to address this issue. (2) Methods: Fungal strains were selected based on bioactivity against CFB, and CFC seed pelletization with fungal conidia was subject to evaluation of control efficacy against CFB. The effective mixture of fungus and chemical insecticide was determined based on safety and joint toxicology tests. (3) Results: The screening of 103 strains from 14 genera identified the Metarhizium anisopliae strain MaGX19S02 (Ma) as the one with the highest virulence. The LC50s of Ma to CFB adult and second instar larvae on day 9 post-treatment were 3.04 × 106 and 27.2 × 106 spores/mL, respectively. In the pot test, the pelletization of CFC seeds with Ma conidia (50/25/12.5 mg in 1 g seed with 4 g fillers) demonstrated significant CFB mortalities (45–82%) 20 days after the larvae were introduced. In the field test, the seed pelletization achieved 57–81% control efficacy 14 days after sowing. Furthermore, the combination of Ma with chlorfenapyr (Chl) demonstrated a synergistic effect against CFB; based on this result, we prepared the mixture formulation of 20% Ma-Chl wettable powder (WP). The assessment of the effects of 20% Ma-Chl WP (500× diluent) against CFB revealed 93.33% mortality in the pot test and 61.3% control efficacy in the field test on day 7 post-treatment. (4) Conclusions: The findings demonstrate the potential of Ma to control CFB in the field. Seed pelletization with Ma conidia effectively controlled CFB larvae and protected CFC seedlings, wherein a mixture formulation of 20% Ma-Chl WP had substantial efficacy in controlling CFB adults. Our research provides new methods for CFB biocontrol.

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Development and evaluation of RNA microsphere‐based RNAi approaches for managing the striped flea beetle (Phyllotreta striolata), a globally destructive pest of Cruciferae crops

Kong,

Xu,

Shen

et al. 2024

Pest Management Science

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BACKGROUNDRNA interference (RNAi) technology has emerged as a promising strategy for species‐specific management of agricultural pests. However, the application of this technology has been significantly hindered by the instability of the interfering RNA molecules in the insect body after ingestion leading to variations in the susceptibility to the RNA triggers across different taxonomic groups of insects. Therefore, it is necessary to develop new approaches that will overcome these challenges associated with the use of RNAi‐based insect pest management strategies. This study explored the use of RNA microspheres (RMS) synthesized via rolling‐circle transcription (RCT) technology as a potential method for managing striped flea beetle (Phyllotreta striolata), a globally destructive pest of Cruciferae crops.RESULTSThe synthesized RMS against the genes encoding reticulocalbin (RMS‐PsRCN) and ribosomal RNA (RMS‐PsrRNA) were highly effective in both silencing their target genes and causing increased P. striolata adult mortality. Relative expression levels of the target genes RMS‐PsRCN and RMS‐PsrRNA were decreased by 74.9% and 68.92%, respectively, in RMS fed adults, compared with the control adults fed RMS‐EGFP. Consequently, the adult mortalities were 81.7% and 73.3% when fed RMS‐PsRCN and RMS‐PsrRNA, respectively, compared with 8.3% in the control adults. Furthermore, movements of adults fed RMS‐PsRCN and RMS‐PsrRNA were decreased by 70.2% and 55.7%, respectively, compared with the control adults.CONCLUSIONSThis study shows the potential of using RMS to suppress the expression of target genes and subsequently produce significant mortality rates and behavioral changes in RMS‐fed adult P. striolata. These findings underscore the promises and viability of using RMS as an effective strategy for gene function studies and species‐specific management of agricultural important insect pests. © 2024 Society of Chemical Industry.

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Silencing of dre4 Contributes to Mortality of Phyllotreta striolata

Cited by 6 publications

References 52 publications

Biology, Ecology, and Management of Flea Beetles in Brassica Crops

Biology, Ecology, and Management of Flea Beetles in Brassica Crops

Screening of Fungal Strains and Formulations of Metarhizium anisopliae to Control Phyllotreta striolata in Chinese Flowering Cabbage

Development and evaluation of RNA microsphere‐based RNAi approaches for managing the striped flea beetle (Phyllotreta striolata), a globally destructive pest of Cruciferae crops

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