Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Alburquerque, Nuria; Faize, Lydia; Burgos, L.

doi:10.1002/ps.4600

“…The transformation approach could consist of infecting apricot leaves for 10 min in a bacterial suspension (cultured for 24 h in a medium containing 500 ¼M AS and diluted to about 107 cfu ml-1), with a co-culture time of 4 days in the presence of l00 ¼M acetosyringone (Petri et al 2004). Recently, an RNAi-mediated gene silencing approach was also used to produce plum rootstocks that seem to be resistant to the crown gall disease but failed to produce the same trait in apricot (Alburquerque et al 2017). The advantage of using genetically engineered rootstocks in rosaceae species is that, rootstocks could be grafted with standard non-transgenic commercial scion cultivars to produce non-genetically modified varieties to address the public's concerns regarding genetically modified fruits (Alburquerque et al 2017).…”

Section: Mechanisms To Improve Apricot Yield Under Environmental Consmentioning

confidence: 99%

Production, pomological and nutraceutical properties of apricot

Moustafa¹,

Cross²

2019

Preprint

0

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Apricot (Prunus sp.) is an important fruit crop worldwide. Despite recent advances in apricot research, much is still to be done to improve its productivity and environmental adaptability. The availability of wild apricot germplasms with economically interesting traits is a strong incentive to increase research panels toward improving its economic, environmental and nutritional characteristics. New technologies and genomic studies have generated a large amount of raw data that the mining and exploitation can help decrypt the biology of apricot and enhance its agronomic values. Here, we outline recent findings in relation to apricot production, pomological and nutraceutical properties. In particular, we retrace its origin from central Asia and the path it took to attain Europe and other production areas around the Mediterranean basin while locating it in the rosaceae family and referring to its genetic diversities and new attempts of classification. The production, nutritional, and nutraceutical importance of apricot are recapped in an easy readable and comparable way. We also highlight and discuss the effects of late frost damages on apricot production over different growth stages, from swollen buds to green fruits formation. Issues related to the length of production season and biotic and abiotic environmental challenges are also discussed with future perspective on how to lengthen the production season without compromising the fruit quality and productivity

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“…The transformation approach could consist of infecting apricot leaves for 10 min in a bacterial suspension (cultured for 24 h in a medium containing 500 1/4M AS and diluted to about 107 cfu ml -1 ), with a co-culture time of 4 days in the presence of l00 M acetosyringone (Petri et al 2004). Recently, an RNAi-mediated gene silencing approach was also used to produce plum rootstocks that seem to be resistant to the crown gall disease but failed to produce the same trait in apricot (Alburquerque et al 2017). The advantage of using genetically engineered rootstocks in rosaceae species is that, rootstocks could be grafted with standard non-transgenic commercial scion cultivars to produce non-genetically modified varieties to address the public's concerns regarding genetically modified fruits (Alburquerque et al 2017).…”

Section: Mechanisms To Improve Apricot Yield Under Environmental Constraintsmentioning

confidence: 99%

Production, pomological and nutraceutical properties of apricot

Moustafa¹,

Cross

²

2018

View full text Add to dashboard Cite

Apricot (Prunus sp.) is an important fruit crop worldwide. Despite recent advances in apricot research, much is still to be done to improve its productivity and environmental adaptability. The availability of wild apricot germplasms with economically interesting traits is a strong incentive to increase research panels toward improving its economic, environmental and nutritional characteristics. New technologies and genomic studies have generated a large amount of raw data that the mining and exploitation can help decrypt the biology of apricot and enhance its agronomic values. Here, we outline recent findings in relation to apricot production, pomological and nutraceutical properties. In particular, we retrace its origin from central Asia and the path it took to attain Europe and other production areas around the Mediterranean basin while locating it in the rosaceae family and referring to its genetic diversities and new attempts of classification. The production, nutritional, and nutraceutical importance of apricot are recapped in an easy readable and comparable way. We also highlight and discuss the effects of late frost damages on apricot production over different growth stages, from swollen buds to green fruits formation. Issues related to the length of production season and biotic and abiotic environmental challenges are also discussed with future perspective on how to lengthen the production season without compromising the fruit quality and productivity.

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“…Both the original and improved protocol have been employed successfully for the introduction of agronomically useful genes into European plum (Alburquerque et al 2017 ; Callahan and Scorza 2007 ; Diaz-Vivancos et al 2013 ; Faize et al 2013 ; García-Almodóvar et al 2015 ; Gonzalez Padilla et al 2003 ; Guseman et al 2017 ; Hily et al 2007 ; Hollender et al 2016 ; Kalariya et al 2011 ; Monticelli et al 2012 ; Nagel et al 2008 ; Petri et al 2008 , 2011 ; Scorza et al 1994 , 1995 ; Srinivasan et al 2012 ; Wang et al 2013b ).…”

Section: Introductionmentioning

confidence: 99%

“…Its expression in Nicotiana tabacum induced resistance to crown gall disease (Alburquerque et al 2012 ). Recently, this construct has been introduced and expressed in European plum, and several transgenic lines, derived from ‘Claudia verde’ hypocotyls, showed a significant reduction in the development of the crown gall disease after infection with the C58 and A281 Agrobacterium strains (Alburquerque et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Current achievements and future directions in genetic engineering of European plum (Prunus domestica L.)

Petri

¹

,

Alburquerque

²

,

Faize

³

et al. 2018

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In most woody fruit species, transformation and regeneration are difficult. However, European plum (Prunus domestica) has been shown to be amenable to genetic improvement technologies from classical hybridization, to genetic engineering, to rapid cycle crop breeding (‘FasTrack’ breeding). Since the first report on European plum transformation with marker genes in the early 90 s, numerous manuscripts have been published reporting the generation of new clones with agronomically interesting traits, such as pests, diseases and/or abiotic stress resistance, shorter juvenile period, dwarfing, continuous flowering, etc. This review focuses on the main advances in genetic transformation of European plum achieved to date, and the lines of work that are converting genetic engineering into a contemporary breeding tool for this species.

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Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Abstract: RNAi-mediated gene silencing can be used for inducing crown gall resistance in plum rootstocks. These could be used to graft non-genetically modified commercial fruit cultivars reducing, or eliminating, the disease symptoms. © 2017 Society of Chemical Industry.

Cited by 13 publications

References 44 publications

Production, pomological and nutraceutical properties of apricot

Production, pomological and nutraceutical properties of apricot

Production, pomological and nutraceutical properties of apricot

Current achievements and future directions in genetic engineering of European plum (Prunus domestica L.)

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