Silencing of IL-6 and STAT3 by siRNA loaded hyaluronate-N,N,N-trimethyl chitosan nanoparticles potently reduces cancer cell progression

Masjedi, Ali; Ahmadi, Armin; Atyabi, Fatemeh; Farhadi, Sareh; Irandoust, Mahzad; Khazaei-Poul, Yalda; Chaleshtari, Mitra Ghasemi; Fathabad, Mahdi Edalati; Baghaei, Masoumeh; Haghnavaz, Navideh; Baradaran, Behzad; Hojjat‐Farsangi, Mohammad; Ghalamfarsa, Ghasem; Sabz, Gholamabas; Hasanzadeh, Sajad; Jadidi‐Niaragh, Farhad

doi:10.1016/j.ijbiomac.2020.01.273

Cited by 64 publications

(29 citation statements)

References 42 publications

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“…Briefly, the ELISA plate was first coated with antibody (150 μl) and incubated for 24 h. The plate was washed with PBS (Tween-20, 0.05 ٪) and then incubated with a blocking buffer for 2 h. After adding 150 μl of standard and sample, it was followed by incubation, and then the plate was washed and incubated for 2 h after adding 150 μl of biotinylated antibody. Then 150 μl of the avidin-biotin-peroxidase solution was added, and incubated for 1 h. Finally, 150 μl of TMB was added and incubated for 1 h. To evaluate the adsorption values, reactions were stopped and then detected by an ELISA reader (Medgenix, BP-800; Biohit) at 460 nm [28] .…”

Section: Methodsmentioning

confidence: 99%

Different T cell related immunological profiles in COVID-19 patients compared to healthy controls

Khesht

Karpisheh

Saeed

et al. 2021

International Immunopharmacology

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In various pathological conditions, cellular immunity plays an important role in immune responses. Among immune cells, T lymphocytes pdomote cellular and humoral responses as well as innate immunity. Therefore, careful investigation of these cells has a significant impact on accurate knowledge in COVID-19 disease pathogenesis. In current research, the frequency and function of various T lymphocytes involved in immune responses examined in SARS‐CoV-2 patients with various disease severity compared to normal subjects. In order to make an accurate comparison among patients with various disease severity, this study was performed on asymptomatic recovered cases (n=20), ICU hospitalized patients (n=30), non-ICU hospitalized patients (n=30), and normal subjects (n=20). To precisely evaluate T cells activity following purification, their cytokine secretion activity was examined. Similarly, immediately after purification of Treg cells, their inhibitory activity on T cells was investigated. The results showed that COVID-19 patients with severe disease (ICU hospitalized patients) not only had a remarkable increase in Th1 and Th17 but also a considerable decrease in Th2 and Treg cells. More importantly, as the IL-17 and IFN-γ secretion was sharply increased in severe disease, the secretion of IL-10 and IL-4 was decreased. Furthermore, the inhibitory activity of Treg cells was reduced in severe disease patients in comparison to other groups. In severe COVID-19 disease, current findings indicate when the inflammatory arm of cellular immunity is significantly increased, a considerable reduction in anti-inflammatory and regulatory arm occurred.

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Section: Methodsmentioning

confidence: 99%

Different T cell related immunological profiles in COVID-19 patients compared to healthy controls

Khesht

Karpisheh

Saeed

et al. 2021

International Immunopharmacology

Self Cite

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“…Finally, gene delivery may also take advantage of NPmediated delivery to avoid the complications with viral vectors, such as activation of the host's immune system and very limited payload capacity [162]. On this point, Masjedi and colleagues [163] utilized modified chitosan-based NPs to encapsulate siRNAs suppressing interleukin-6 and signal transducer and activator of transcription 3. These mechanisms support tumor progression by promoting proliferation, antiapoptotic factors, and drug resistance.…”

Section: Nms-facilitated Treatment Modalitiesmentioning

confidence: 99%

“…Treatment of CAMs grafted with murine cancer cells with the siRNA-loaded NPs resulted in reduced tumor size, weight, and number of blood microvessels. Furthermore, mRNA expressions of tumor and angiogenesis promoting genes FGF, transforming growth factor-β (TGF-β), and VEGF were downregulated [163].…”

Section: Nms-facilitated Treatment Modalitiesmentioning

confidence: 99%

Tumor grafted – chick chorioallantoic membrane as an alternative model for biological cancer research and conventional/nanomaterial-based theranostics evaluation

Mapanao

Pei

Sarogni

et al. 2021

Expert Opinion on Drug Metabolism & Toxicology

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Introduction: Advancements in cancer management and treatment are associated with strong preclinical research data, in which reliable cancer models are demanded. Indeed, inconsistent preclinical findings and stringent regulations following the 3Rs principle of reduction, refinement, and replacement of conventional animal models currently pose challenges in the development and translation of efficient technologies. The chick embryo chorioallantoic membrane (CAM) is a system for the evaluation of treatment effects on the vasculature, therefore suitable for studies on angiogenesis. Apart from vascular effects, the model is now increasingly employed as a preclinical cancer model following tumorgrafting procedures. Areas covered: The broad application of CAM tumor model is highlighted along with the methods for analyzing the neoplasm and vascular system. The presented and cited investigations focus on cancer biology and treatment, encompassing both conventional and emerging nanomaterial-based modalities. Expert opinion: The CAM tumor model finds increased significance given the influences of angiogenesis and the tumor microenvironment in cancer behavior, then providing a qualified miniature system for oncological research. Ultimately, the establishment and increased employment of such a model may resolve some of the limitations present in the standard preclinical tumor models, thereby redefining the preclinical research workflow.

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“…The simultaneous delivery of these siRNAs has exhibited the excellent downregulation of IL‐6 and STAT 3 in three different murine‐derived cancer cell lines such as 4T1 breast cancer, CT‐26 colon cancer, and B16F10 melanoma and elicited the synergistic anticancer effects. [ 85 ] The combination of small molecule drug icaritin, known to induce autophagy, and doxorubicin, can promote the synergistic ICD. Huang's group explored this strategy by employing PLGA‐PEG polymer‐based nanoparticles for co‐delivery of these two drugs at an optimized ratio.…”

Section: Self‐assembled Multifunctional Nanomaterials For Combinatorimentioning

confidence: 99%

Engineered Multifunctional Nano‐ and Biological Materials for Cancer Immunotherapy

Brouillard

Deshpande

Kulkarni

2021

Adv Healthcare Materials

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Cancer immunotherapy is set to emerge as the future of cancer therapy. However, recent immunotherapy trials in different cancers have yielded sub‐optimal results, with durable responses seen in only a small fraction of patients. Engineered multifunctional nanomaterials and biological materials are versatile platforms that can elicit strong immune responses and improve anti‐cancer efficacy when applied to cancer immunotherapy. While there are traditional systems such as polymer‐ and lipid‐based nanoparticles, there is a wide variety of other materials with inherent and additive properties that can allow for more potent activation of the immune system. By synthesizing and applying multifunctional strategies, it allows for a more extensive and more effective repertoire of tools to use in the wide variety of situations that cancer presents itself. Here, several types of nanoscale and biological material strategies and platforms that provide their inherent benefits for targeting and activating multiple aspects of the immune system are discussed. Overall, this review aims to provide a comprehensive understanding of recent advances in the field of multifunctional cancer immunotherapy and trends that pave the way for more diverse and tactical regression of tumors through soliciting responses by either the adaptive or innate immune system, and even both simultaneously.

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Silencing of IL-6 and STAT3 by siRNA loaded hyaluronate-N,N,N-trimethyl chitosan nanoparticles potently reduces cancer cell progression

Cited by 64 publications

References 42 publications

Different T cell related immunological profiles in COVID-19 patients compared to healthy controls

Different T cell related immunological profiles in COVID-19 patients compared to healthy controls

Tumor grafted – chick chorioallantoic membrane as an alternative model for biological cancer research and conventional/nanomaterial-based theranostics evaluation

Engineered Multifunctional Nano‐ and Biological Materials for Cancer Immunotherapy

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