Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203

Wang, Shilei; Hui, Yuzuo; Li, Xiaoming; Jia, Qingbin

doi:10.3727/096504017x14965095236521

Cited by 63 publications

(34 citation statements)

References 40 publications

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“…The overall prognosis of patients with advanced glioma is poor (1,2). In recent years, a number of studies have focused on investigating the pathogenesis of glioma (3)(4)(5). However, the molecular mechanisms underlying glioma progression remain largely unknown; therefore, there is an urgent requirement to identify novel and effective diagnostic and therapeutic targets for this disease.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑599 suppresses glioma progression by targeting RAB27B

et al. 2018

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MicroRNAs (miRNAs/miRs) serve tumor promoting or suppressive roles in different human cancer types, including glioma; however, the regulatory underlying mechanism by which miR-599 affects glioma progression remains largely unknown. The aim of the present study was to investigate the expression of miR-599 in glioma, as well as the underlying regulatory mechanism. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to examine mRNA and protein expression, respectively. MTT, wound healing and transwell assays were conducted to study cell proliferation, migration and invasion, respectively. A dual-luciferase reporter gene assay was used to confirm the targeting association between miR-599 and Ras-related protein Rab-27B (hereafter RAB27B). In the present study, miR-599 expression was observed to be significantly downregulated in human glioma tissues and cell lines, when compared with normal brain tissues and normal human astrocyte cells, respectively. Low miR-599 expression was significantly associated with glioma progression. Ectopic expression of miR-599 caused a significant reduction in the proliferation, migration and invasion of U-87MG Uppsala and U251 cells. Bioinformatics analysis and dual-luciferase reporter gene assay data identified that RAB27B was a direct target gene of miR-599. The mRNA and protein expression of RAB27B was significantly downregulated following miR-599 overexpression in U-87MG Uppsala and U251 cells. Rescue experiments demonstrated that RAB27B rescued the miR-599-induced inhibition of glioma cell growth, migration and invasion. In addition, RAB27B expression was significantly upregulated in glioma tissues and cell lines, and the expression levels of RAB27B were inversely correlated with miR-599 levels in glioma tissues; therefore, the present study demonstrated that miR-599 exerts a tumor-suppressive role in glioma progression by targeting RAB27B, indicating that miR-599 may be used as a potential candidate for glioma treatment.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑599 suppresses glioma progression by targeting RAB27B

et al. 2018

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“…MicroRNAs (miRs), a class of small non-coding RNAs, can directly bind to the 3' untranslated region (UTR) of their target mRNAs, and cause RNA degradation or translation inhibition (7,8). Through mediating the expression of their target genes, miRs are involved in various cellular biological processes, including cell proliferation, differentiation, migration and invasion, as well as tumorigenesis (9)(10)(11). A large number of miRs have been demonstrated to have promoting or suppressive roles in various human cancer types, including cervical cancer, and certain miRs are strongly associated with HPV (12,13).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑130a regulated by HPV18 E6 promotes proliferation and invasion of cervical cancer cells by targeting TIMP2

et al. 2019

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Human papillomaviruses (HPVs) have important roles in the development and progression of cervical cancer, but the underlying mechanisms are yet to be fully elucidated. MicroRNA-130a (miR-130a) has previously been reported to promote cervical cancer growth. However, the underlying molecular mechanisms by which miR-130a promotes cervical cancer progression have remained largely elusive. In the present study, polymerase chain reaction and western blot analyses were performed to examine the expression levels of miR-130a and associated proteins. A wound healing assay and a Transwell assay were applied to study cell migration and invasion. A luciferase reporter gene assay was performed to confirm the targeting associations of miR-130a. It was observed that miR-130a was significantly upregulated in cervical cancer tissues compared with that in adjacent non-tumorous tissues. High expression of miR-130a was significantly associated with lymph node metastasis and an advanced clinical stage of cervical cancer. Furthermore, the expression of miR-130a was also higher in HPV(+) cervical cancer cell lines compared with that in HPV(-) cells. Knockdown of HPV18 E6 significantly inhibited the expression of miR-130a in HeLa cervical cancer cells. Furthermore, knockdown of miR-130a reduced the migration and invasion of HeLa cells. Tissue inhibitor of metalloproteinase 2 (TIMP2), an antagonist of matrix metalloproteinase 2 (MMP2), was identified as a novel, direct target gene of miR-130a. The expression of TIMP2 was negatively mediated by miR-130a, and HPV18 E6 inhibited the expression of TIMP2 in HeLa cells. Furthermore, knockdown of TIMP2 rescued the suppressive effects of miR-130a downregulation on the migration and invasion of HeLa cells. In summary, the present study suggests that HPV18 E6 promotes the expression of miR-130a, which further inhibits the expression of TIMP2 and promotes cervical cancer cell invasion. Therefore, HPV/miR-130a/TIMP2 signaling may be a potential target for the prevention of cervical cancer metastasis.

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“…microRNAs (miRNAs or miRs), a class of non-coding RNAs that are ~22 nucleotides in length, are important gene regulators that bind to the 3'-untranslated region (UTR) of their target mRNAs, resulting in protein translation inhibition or mRNA degradation (6)(7)(8). A large number of miRNAs have been reported to regulate the expression of genes that are associated with physiological and pathological processes, including cell growth, proliferation, differentiation, apoptosis, migration, angiogenesis and tumourigenesis, among others (9)(10)(11)(12). In recent years, a number of miRs, such as miR-124 (13), miR-21 (14), miR-5195 (15), miR-138 (16), and miR-576 (17) have been demonstrated to have key roles in bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4

et al. 2018

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Dysfunction of microRNAs (miRs) has been implicated in the development and progression of various human cancers. Our previous study demonstrated that miR-101 inhibited bladder cancer cell proliferation and invasion through inhibition of c-FOS expression. As an miR generally has many targets, other targets of miR-101 may also serve important roles in bladder cancer progression. Reverse transcription-quantitative polymerase chain reaction and western blot analyses were used to examine mRNA and protein expression, respectively. Wound healing and Transwell assays were conducted to study cell migration and invasion, respectively. The luciferase reporter gene assay was performed to verify one of the targets of miR-101. The data in the present study indicate that the expression of miR-101 is significantly reduced in bladder cancer tissues compared with that in adjacent non-tumour tissues. In addition, miR-101 expression is also downregulated in bladder cancer cell lines compared with that in normal bladder epithelial cells. Furthermore, low expression of miR-101 was significantly associated with tumour metastasis, advanced clinical stage, and poor prognosis in bladder cancer. Frizzled class receptor 4 (FZD4) was identified as a novel target of miR-101 in bladder cancer cells. The expression of FZD4 was significantly upregulated in bladder cancer tissues and cell lines. Both miR-101 overexpression and FZD4 inhibition caused a significant reduction of the migration and invasion of bladder cancer cells, whereas overexpression of FZD4 reversed the suppressive effects of miR-101 on bladder cancer cell migration and invasion. In conclusion, it was demonstrated that miR-101 downregulation is associated with bladder cancer progression and that miR-101 can inhibit bladder cancer cell migration and invasion via directly targeting FZD4. The present study expands the understanding of the molecular mechanisms underlying bladder cancer progression.

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Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203

Cited by 63 publications

References 40 publications

MicroRNA‑599 suppresses glioma progression by targeting RAB27B

MicroRNA‑599 suppresses glioma progression by targeting RAB27B

MicroRNA‑130a regulated by HPV18 E6 promotes proliferation and invasion of cervical cancer cells by targeting TIMP2

MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4

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