Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells

Liao, Yixiang; Zeng, Jinmin; Zhou, Jiajie; Yang, Guanghua; Ding, Kun; Zhang, Xian‐Jue

doi:10.3892/mmr.2016.5127

Cited by 19 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have confirmed the anti-apoptotic effects of RTKN2 on several cancer types including HCC and bladder cancer ( 5 , 6 ). Consistent with these observations, the results of the present study demonstrated that silencing of RTKN2 markedly induced the apoptosis of human colon cancer cells.…”

Section: Discussionmentioning

confidence: 64%

“…Wei et al ( 5 ) reported the overexpression of RTKN2 in the majority of hepatocellular carcinoma (HCC) patients and demonstrated an association between RTKN2 expression and proliferation, apoptosis and metastatic progression. Similarly, Liao et al ( 6 ) reported the anti-apoptotic effects of RTKN2 in bladder cancer cells. Furthermore, the overexpression of RTKN2 reduced viability and increased sensitivity to 25-OHC ( 7 ), which is directly associated with apoptosis in hematopoietic and leukemic cells ( 8 – 10 ).…”

Section: Introductionmentioning

confidence: 83%

“…Furthermore, the overexpression of RTKN2 reduced viability and increased sensitivity to 25-OHC ( 7 ), which is directly associated with apoptosis in hematopoietic and leukemic cells ( 8 – 10 ). Although the RTKN2 gene is associated with several cancer types, including HCC, bladder and breast cancer ( 5 , 6 , 11 ), the expression pattern and biological functions of RTKN2 in human colon cancer have yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells

Pang

Shi

et al. 2017

Oncol Lett

View full text Add to dashboard Cite

Colon cancer is one of the most common malignant tumors in the human body, ranking second as a gastrointestinal tumor. It has a high incidence in Europe, America and China and more than 1 million new cases of colon cancer are reported worldwide each year. The incidence of colon cancer in China has increased from 12/0.1 million in the early 1970s to 56/0.1 million at present with an annual growth rate of 4.2%, which far exceeds the international level (2%). Rhotekin (RTKN) 2, a Rho-guanosine triphosphatase (GTPase) effector, has been reported to be anti-apoptotic. However, the molecular mechanism underlying the biological function of RTKN2 in colon cancer remains unknown. The present study investigated whether the mRNA expression level of RTKN2 was markedly higher in 30 human colon cancer specimens compared with adjacent non-cancerous tissues. The results showed that the protein expression level of RTKN2 was significantly higher in SW480 and HCT116 cells, compared with HIEC cells. Knockdown of RTKN2 in the SW480 and HCT116 colon cancer cells, by lentivirus-mediated RNA interference led to the notable inhibition of cell proliferation and cell cycle progression, by reducing the expression levels of the PCDA, Cyclin D1 and c-myc cell cycle-associated proteins. The inhibitory effect of RTKN2 silencing on the proliferation of colon cancer cells may be partially realized by inhibiting the Wnt/β-catenin signaling pathway. Furthermore, the silencing of RTKN2 in the cells induced apoptosis by reducing the expression level of Bax and increasing the expression level of Bcl2. These results show that RTKN2 is involved in the carcinogenesis and progression of human colon cancer, indicating that RTKN2 may be a molecular target in colon cancer therapy.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells

Pang

Shi

et al. 2017

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…Therefore, the reduction of BCL2L15 may be involved in this effect. Silencing of RTKN2 suppressed proliferation and induced apoptosis and cell cycle arrest in human bladder cancer cells (Liao et al, ). RTKN‐mediated NFκB activation confers resistance to apoptosis in gastric cancer (Liu, Wang, Chi, Wu, & Chen, ).…”

Section: Discussionmentioning

confidence: 99%

Section: Data Verification By Real-time Pcrmentioning

confidence: 98%

Gene expression profiling reveals the plausible mechanisms underlying the antitumor and antimetastasis effects of Andrographis paniculata in esophageal cancer

Yue

Lee

et al. 2018

Phytotherapy Research

View full text Add to dashboard Cite

Esophageal cancer (EC) is a seriously invasive malignancy with high mortality and poor prognosis. Metastasis of EC is the major cause of mortality. Our studies previously demonstrated that a herbal medicine Andrographis paniculata (AP) significantly suppressed EC growth and metastasis in vitro and in vivo. However, the underlying mechanisms responsible for these effects have not yet been systematically elucidated. In this context, gene expression profiling of AP-treated squamous EC cells (EC-109) was performed to reveal the regulatory mechanisms of AP in antitumor and antimetastasis signaling pathways using gene expression microarray analysis. Differentially expressed genes were identified by Affymetrix Gene Chip, followed by the real-time polymerase chain reaction validation. The results showed that the canonical pathways were significantly regulated by AP treatment, including multiple genes related to proliferation, apoptosis, intercellular adhesion, metastatic processes, and drug resistance, such as WNT, TGF-β, MAPK and ErbB signaling pathways, and ATP-binding cassette transporter subfamily members. This genomic study emerges candidate molecular targets and pathways to reveal the mechanisms involved in AP's effects, which provides scientific evidence to support the clinical application of AP in EC treatment.

show abstract

LncRNAs exhibit subtype-specific expression, survival associations, and cancer-promoting effects in breast cancer

Wasson,

Venkatesh,

Cahill

et al. 2024

Gene

View full text Add to dashboard Cite

Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells

Cited by 19 publications

References 26 publications

Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells

Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells

Gene expression profiling reveals the plausible mechanisms underlying the antitumor and antimetastasis effects of Andrographis paniculata in esophageal cancer

LncRNAs exhibit subtype-specific expression, survival associations, and cancer-promoting effects in breast cancer

Contact Info

Product

Resources

About