Silencing of <scp>STRN</scp>4 suppresses the malignant characteristics of cancer cells

Wong, Meihong; Hyodo, Toshinori; Asano, Eri; Funasaka, Kohei; Miyahara, Ryoji; Hirooka, Yoshiki; Goto, Hidemi; Hamaguchi, Michinari; Senga, Takeshi

doi:10.1111/cas.12541

Cited by 40 publications

(54 citation statements)

References 38 publications

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“…2B). Reduced cell proliferation can be induced by a delay in cell cycle progression or by the promotion of cellular apoptosis (21). To determine how TRIP13 depletion suppressed cell proliferation, a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and an EdU incorporation assay were performed.…”

Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

“…To determine how TRIP13 depletion suppressed cell proliferation, a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and an EdU incorporation assay were performed. The TUNEL assay detects fragmented DNA induced by apoptosis, while the EdU incorporation assay evaluates cell cycle progression (21). Cells that progress through the S phase incorporate the thymidine analog EdU; thus, proliferating cells can be detected based on the level of EdU incorporated (21).…”

Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

“…The TUNEL assay detects fragmented DNA induced by apoptosis, while the EdU incorporation assay evaluates cell cycle progression (21). Cells that progress through the S phase incorporate the thymidine analog EdU; thus, proliferating cells can be detected based on the level of EdU incorporated (21). As shown in Fig.…”

Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

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TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion

et al. 2016

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Abstract. Thyroid hormone receptor interactor 13 (TRIP13) is a member of the ATPases associated with various cellular activities family of proteins and is highly conserved in a wide range of species. Recent studies have demonstrated that TRIP13 is critical for the inactivation of the spindle assembly checkpoint and is associated with the progression of certain cancers. In the present study, the role of TRIP13 in colorectal cancer (CRC) was examined. Reverse transcription-quantitative polymerase chain reaction analysis revealed that TRIP13 messenger RNA was highly expressed in multiple CRC tissues. The depletion of TRIP13 in CRC cells suppressed cell proliferation, migration and invasion. To determine whether the catalytic activity of TRIP13 was critical for cancer progression, an inactive mutant of TRIP13 was expressed in CRC cells. The invasion of cancer cells that expressed the mutant TRIP13 was significantly reduced compared with that of the wild type TRIP13-expressing cancer cells. These results indicate that TRIP13 could be a potential target for CRC treatment. IntroductionThe ATPases associated with various cellular activities (AAA+) family of proteins comprises a functionally different group of enzymes that are involved in an array of cellular processes, including protein degradation, protein-complex disassembly and DNA replication (1). The AAA+ proteins are present in all kingdoms, and are characterized by the presence of conserved AAA+ domains that contain Walker A and Walker B motifs, followed by a conserved region called second region of homology (2). The majority of AAA+ proteins form hexamers to exert their biological functions. The chemical energy generated by adenosine triphosphate (ATP) hydrolysis by this family of proteins induces conformational changes in the substrate proteins to modulate their functions (3,4).A previous study reported that certain AAA+ enzymes are associated with tumor progression (5). For example, reptin [also known as RuvB-like (RUVBL) 2] and pontin (RUVBL1) have been demonstrated to be overexpressed in several tumors (5). These proteins interact with c-Myc or β-catenin, thus modulating their transcriptional activities to promote tumor progression (6). AAA+ proteins hydrolyze ATP, and thus, chemical inhibitors that disrupt the activities of cancer-associated AAA+ proteins may represent promising anti-cancer drugs.Thyroid hormone receptor interactor 13 (TRIP13, also known as 16E1BP and pachytene checkpoint 2) is a member of the AAA+ family proteins and is conserved in a wide range of species (7). It was first identified as a protein that interacts with human papilloma virus E1 proteins by a yeast two-hybrid analysis, but the physiological function of the interaction remains unknown (7). Accumulating studies have demonstrated that TRIP13 plays pivotal roles in meiotic recombination and DNA repair in plants, yeast, worms and mice (8-12). TRIP13 forms a stable hexameric ring, and ATP binding, as well as ATP hydrolysis, are critical for the function of the protein (13). Previous...

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Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

Section: Depletion Of Trip13 Suppresses Cell Proliferationmentioning

confidence: 99%

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TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion

et al. 2016

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“…DJ-1 has also been associated with cancer cell survival and metastasis. Recently, it has been shown that downregulation of striatin 4/zinedin, the third member of striatin family, reduces cancer cell survival [28]. We thus asked whether SG2NA being a scaffold for both DJ-1 and Akt, promotes cancer cell proliferation.…”

Section: Downregulation Of Sg2na Decreases Cancer Cells Growthmentioning

confidence: 98%

SG2NA enhances cancer cell survival by stabilizing DJ-1 and thus activating Akt

Tanti

Pandey

Goswami

2015

Biochemical and Biophysical Research Communications

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“…Out of the 15 genes, 9 of them have been reported to be associated with cancer. These include genes which are reported to be related with breast cancer: PMP22 (Winslow et al, 2013), AIM2 (Liu et al, 2015), colorectal cancer: CNGA3 (Shaikh et al, 2015), ovarian cancer HOXB8 (Stavnes et al, 2013), liver cancer HNF4A (Ning et al, 2010), pancreatic cancer PCDHB2 (Carter et al, 2010), and cancer progression STRN4 (Wong et al, 2014), CHCHD4 (Yang et al, 2012), and VHL (Kim and Kaelin, 2004), where the number in the parenthesis is the rank of p-value among the 15 genes.…”

Section: Application To Dna Methylation Data Of Ovarian Cancermentioning

confidence: 99%

PLEMT: A Novel Pseudolikelihood-Based EM Test for Homogeneity in Generalized Exponential Tilt Mixture Models

Hong

Ning

Wang

et al. 2017

Journal of the American Statistical Association

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Motivated by analyses of DNA methylation data, we propose a semiparametric mixture model, namely the generalized exponential tilt mixture model, to account for heterogeneity between differentially methylated and non-differentially methylated subjects in the cancer group, and capture the differences in higher order moments (e.g. mean and variance) between subjects in cancer and normal groups. A pairwise pseudolikelihood is constructed to eliminate the unknown nuisance function. To circumvent boundary and non-identifiability problems as in parametric mixture models, we modify the pseudolikelihood by adding a penalty function. In addition, the test with simple asymptotic distribution has computational advantages compared with permutation-based test for high-dimensional genetic or epigenetic data. We propose a pseudolikelihood based expectation–maximization test, and show the proposed test follows a simple chi-squared limiting distribution. Simulation studies show that the proposed test controls Type I errors well and has better power compared to several current tests. In particular, the proposed test outperforms the commonly used tests under all simulation settings considered, especially when there are variance differences between two groups. The proposed test is applied to a real data set to identify differentially methylated sites between ovarian cancer subjects and normal subjects.

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Silencing of STRN4 suppresses the malignant characteristics of cancer cells

Cited by 40 publications

References 38 publications

TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion

TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion

SG2NA enhances cancer cell survival by stabilizing DJ-1 and thus activating Akt

PLEMT: A Novel Pseudolikelihood-Based EM Test for Homogeneity in Generalized Exponential Tilt Mixture Models

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